ID F6U4P2_HORSE Unreviewed; 740 AA.
AC F6U4P2;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 3.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=6-phosphofructokinase {ECO:0000256|PIRNR:PIRNR000533};
DE EC=2.7.1.11 {ECO:0000256|PIRNR:PIRNR000533};
DE AltName: Full=Phosphohexokinase {ECO:0000256|PIRNR:PIRNR000533};
GN Name=PFKP {ECO:0000313|Ensembl:ENSECAP00000018673.3,
GN ECO:0000313|VGNC:VGNC:21342};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000018673.3, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000018673.3, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000018673.3,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000018673.3}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000018673.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-
CC bisphosphate + H(+); Xref=Rhea:RHEA:16109, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:32966, ChEBI:CHEBI:57634,
CC ChEBI:CHEBI:456216; EC=2.7.1.11;
CC Evidence={ECO:0000256|ARBA:ARBA00000432,
CC ECO:0000256|PIRNR:PIRNR000533};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 3/4.
CC {ECO:0000256|ARBA:ARBA00004679, ECO:0000256|PIRNR:PIRNR000533}.
CC -!- SIMILARITY: Belongs to the phosphofructokinase type A (PFKA) family.
CC ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E"
CC sub-subfamily. {ECO:0000256|PIRNR:PIRNR000533}.
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DR AlphaFoldDB; F6U4P2; -.
DR Ensembl; ENSECAT00000022588.4; ENSECAP00000018673.3; ENSECAG00000020055.4.
DR VGNC; VGNC:21342; PFKP.
DR GeneTree; ENSGT00940000155002; -.
DR HOGENOM; CLU_011053_0_0_1; -.
DR TreeFam; TF300411; -.
DR UniPathway; UPA00109; UER00182.
DR Proteomes; UP000002281; Chromosome 29.
DR Bgee; ENSECAG00000020055; Expressed in retina and 23 other cell types or tissues.
DR ExpressionAtlas; F6U4P2; baseline.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0003872; F:6-phosphofructokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.450; -; 3.
DR Gene3D; 3.40.50.460; Phosphofructokinase domain; 2.
DR InterPro; IPR009161; 6-Pfructokinase_euk.
DR InterPro; IPR022953; ATP_PFK.
DR InterPro; IPR015912; Phosphofructokinase_CS.
DR InterPro; IPR000023; Phosphofructokinase_dom.
DR InterPro; IPR035966; PKF_sf.
DR NCBIfam; TIGR02478; 6PF1K_euk; 1.
DR PANTHER; PTHR13697:SF5; ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE, PLATELET TYPE; 1.
DR PANTHER; PTHR13697; PHOSPHOFRUCTOKINASE; 1.
DR Pfam; PF00365; PFK; 2.
DR PIRSF; PIRSF000533; ATP_PFK_euk; 2.
DR PRINTS; PR00476; PHFRCTKINASE.
DR SUPFAM; SSF53784; Phosphofructokinase; 2.
DR PROSITE; PS00433; PHOSPHOFRUCTOKINASE; 2.
PE 1: Evidence at protein level;
KW Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR000533};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152, ECO:0000256|PIRNR:PIRNR000533};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|PIRNR:PIRNR000533};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000533};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Proteomics identification {ECO:0007829|PeptideAtlas:F6U4P2};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000533}.
FT DOMAIN 28..333
FT /note="Phosphofructokinase"
FT /evidence="ECO:0000259|Pfam:PF00365"
FT DOMAIN 511..647
FT /note="Phosphofructokinase"
FT /evidence="ECO:0000259|Pfam:PF00365"
SQ SEQUENCE 740 AA; 81087 MW; 17F1B230D42920F0 CRC64;
MDDQDVSANP RGSFRQFLEH LSGAGKAIGV LTSGGDAQGM NAAVRAVVRM GIYVGAKVYF
IYEGYQGMVD GGSNIVEADW ESVSSILQVG GTIIGSARCK AFRSREGRLQ AARNLVQLGI
TNLCVIGGDG SLTGANIFRK EWSGLLEELA QNGQINKEEV EKYSYLNVVG MVGSIDNDFC
GTDMTIGTDS ALHRIIEVID AIMTTAQSHQ RTFVLEVMGR HCGYLALVSA LACGADWVFL
PESPPEEGWQ ENMCVKLSEN RARKKRLNII IVAEGAIDTQ NKPITSEQIK ELVVTQLGYD
TRVTILGHVQ RGGTPSAFDR ILASRMGVEA VVALLQATPE TPACVVSLSG NQAVRLPLVK
CVQMTQDVQK AMDERRFKDA VQLRGRSFEN NLNTYKRLAI KMPDTQIPKS NCNVGVINVG
APAAGMNAAV RAAVRVGIAD GHKMFAVYDG FEGFAKGQIK EIGWGDVGGW TGQGGSILGT
KRTLPGKYLT EIAGQMRTHS INALLIIGGF ETCDRIKQSA SGTKRRVFII ETMGGYCGYL
ANMGGLAAGA DAAYIFEEPF DIRDLQANVE HLTEKMKTTI QRGLVLRNES CSENYTTDFI
YQLYSEEGKG VFDCRKNVLG HMQQGGAPSP FDRNFGTKMS ARAMQWITTK LKESQGKGRR
FVSDDSICVL GISKRNLVFR PVAELKKETD FEHRIPKEQW WLKLRPLMKI LAKYKASFEV
SDSEELEHVH HRGHEEPAAI
//
