ID F6UEZ5_CIOIN Unreviewed; 759 AA.
AC F6UEZ5;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 2.
DT 27-MAR-2024, entry version 62.
DE RecName: Full=Choline O-acetyltransferase {ECO:0000256|ARBA:ARBA00040495};
DE EC=2.3.1.6 {ECO:0000256|ARBA:ARBA00039091};
GN Name=chat {ECO:0000313|Ensembl:ENSCINP00000005239.3};
OS Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis).
OC Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC Cionidae; Ciona.
OX NCBI_TaxID=7719 {ECO:0000313|Ensembl:ENSCINP00000005239.3, ECO:0000313|Proteomes:UP000008144};
RN [1] {ECO:0000313|Proteomes:UP000008144}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12481130; DOI=10.1126/science.1080049;
RA Dehal P., Satou Y., Campbell R.K., Chapman J., Degnan B., De Tomaso A.,
RA Davidson B., Di Gregorio A., Gelpke M., Goodstein D.M., Harafuji N.,
RA Hastings K.E., Ho I., Hotta K., Huang W., Kawashima T., Lemaire P.,
RA Martinez D., Meinertzhagen I.A., Necula S., Nonaka M., Putnam N., Rash S.,
RA Saiga H., Satake M., Terry A., Yamada L., Wang H.G., Awazu S., Azumi K.,
RA Boore J., Branno M., Chin-Bow S., DeSantis R., Doyle S., Francino P.,
RA Keys D.N., Haga S., Hayashi H., Hino K., Imai K.S., Inaba K., Kano S.,
RA Kobayashi K., Kobayashi M., Lee B.I., Makabe K.W., Manohar C., Matassi G.,
RA Medina M., Mochizuki Y., Mount S., Morishita T., Miura S., Nakayama A.,
RA Nishizaka S., Nomoto H., Ohta F., Oishi K., Rigoutsos I., Sano M.,
RA Sasaki A., Sasakura Y., Shoguchi E., Shin-i T., Spagnuolo A., Stainier D.,
RA Suzuki M.M., Tassy O., Takatori N., Tokuoka M., Yagi K., Yoshizaki F.,
RA Wada S., Zhang C., Hyatt P.D., Larimer F., Detter C., Doggett N.,
RA Glavina T., Hawkins T., Richardson P., Lucas S., Kohara Y., Levine M.,
RA Satoh N., Rokhsar D.S.;
RT "The draft genome of Ciona intestinalis: insights into chordate and
RT vertebrate origins.";
RL Science 298:2157-2167(2002).
RN [2] {ECO:0000313|Ensembl:ENSCINP00000005239.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh)
CC from acetyl CoA and choline at cholinergic synapses.
CC {ECO:0000256|ARBA:ARBA00037088}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + choline = acetylcholine + CoA;
CC Xref=Rhea:RHEA:18821, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.6;
CC Evidence={ECO:0000256|ARBA:ARBA00036720};
CC -!- SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.
CC {ECO:0000256|ARBA:ARBA00005232, ECO:0000256|RuleBase:RU003801}.
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DR EMBL; EAAA01000214; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; F6UEZ5; -.
DR STRING; 7719.ENSCINP00000005239; -.
DR Ensembl; ENSCINT00000005239.3; ENSCINP00000005239.3; ENSCING00000002571.3.
DR GeneTree; ENSGT01060000248595; -.
DR HOGENOM; CLU_013513_3_0_1; -.
DR InParanoid; F6UEZ5; -.
DR OMA; MAMSSYE; -.
DR TreeFam; TF313836; -.
DR Proteomes; UP000008144; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0004102; F:choline O-acetyltransferase activity; IBA:GO_Central.
DR GO; GO:0008292; P:acetylcholine biosynthetic process; IBA:GO_Central.
DR GO; GO:0007626; P:locomotory behavior; IEA:Ensembl.
DR GO; GO:0007274; P:neuromuscular synaptic transmission; IBA:GO_Central.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.30.559.70; Choline/Carnitine o-acyltransferase, domain 2; 1.
DR InterPro; IPR000542; Carn_acyl_trans.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR039551; Cho/carn_acyl_trans.
DR InterPro; IPR042231; Cho/carn_acyl_trans_2.
DR PANTHER; PTHR22589; CARNITINE O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR22589:SF14; CHOLINE O-ACETYLTRANSFERASE; 1.
DR Pfam; PF00755; Carn_acyltransf; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR PROSITE; PS00439; ACYLTRANSF_C_1; 1.
DR PROSITE; PS00440; ACYLTRANSF_C_2; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003801};
KW Neurotransmitter biosynthesis {ECO:0000256|ARBA:ARBA00022979};
KW Reference proteome {ECO:0000313|Proteomes:UP000008144};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003801}.
FT DOMAIN 31..612
FT /note="Choline/carnitine acyltransferase"
FT /evidence="ECO:0000259|Pfam:PF00755"
FT ACT_SITE 338
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600542-1"
SQ SEQUENCE 759 AA; 85861 MW; 12D5D33F804D747D CRC64;
MPGALHQKPL EKRSSRLNIQ KLSCSTSLPS LPLPDLQETL EQYLLCIKAL VPESQFEQTK
EIVESFGATG GVGEAIQRRL EECAKTKVNW AYEWWLNDMY LTNPASLPIN SNPGMVLPKQ
NFEDQLSMLQ YAAKITSGIL DFKVILDARA LPVDVARGQL AGQAMCMEQY YRLLNSYRLP
GYTRDKLVSL KSVQSDEPEH VIVVCNNQFF VVPVMMGNYR LSEAEFVSQF QRILDAAHDP
AEHNPPVGVL TSLKRPDWAE AREIMMSEPI NRLSLDYIEQ SIFILCLDKS LENEDDVTMA
HQMLYGGGPE KNSCNRWYDK TIQLIIGSDG KTGLSYEHSA SEGIAVTALI EHLLKYIALQ
EKKGAYRSNS SIVLPSPRRL QWRLNPSVNQ YVEIGKAKNR HVANSLDLEI LRYTNYGKDF
IKRQNMSPDA YVQVCLQITY YKLYKRLVST YESASTRRFR EGRVDNIRAA TVQALELAKG
LTEPDESGKI KMDAKKMLLL RRAVKAQTDF TIAAITGHGI DCHLLGLKQM ADEMGLPTPG
IFKDSSYELS NYFQLSTSQI PTSTDSFMCY GPVVPDGYGV SYNPHSNSII FCTASFRTCP
STSSEKFVAA LVESLHIMKE ICVQWNRRRS SSLEHLSIHR KLSLHRRVDA RSYSVDCDAL
REEFSAESDS SDNEVLEKKK ILKKQKSFTT TRMIKRKLER RVSLISNLNA HHRGNSKCGI
VDKTQTHTSS NVESSQIPCD LMKLLRQESL NQKNNITIS
//
