ID F6YCX7_HORSE Unreviewed; 697 AA.
AC F6YCX7;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 27-JUL-2011, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=procollagen-proline 3-dioxygenase {ECO:0000256|ARBA:ARBA00012262};
DE EC=1.14.11.7 {ECO:0000256|ARBA:ARBA00012262};
GN Name=P3H2 {ECO:0000313|Ensembl:ENSECAP00000007201.1,
GN ECO:0000313|VGNC:VGNC:21114};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000007201.1, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000007201.1, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000007201.1,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000007201.1}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000007201.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + L-prolyl-[collagen] + O2 = CO2 + succinate +
CC trans-3-hydroxy-L-prolyl-[collagen]; Xref=Rhea:RHEA:22872, Rhea:RHEA-
CC COMP:11676, Rhea:RHEA-COMP:11678, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:50342, ChEBI:CHEBI:85428; EC=1.14.11.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024148};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- SIMILARITY: Belongs to the leprecan family.
CC {ECO:0000256|ARBA:ARBA00006487}.
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DR RefSeq; XP_001500110.1; XM_001500060.3.
DR AlphaFoldDB; F6YCX7; -.
DR STRING; 9796.ENSECAP00000007201; -.
DR PaxDb; 9796-ENSECAP00000007201; -.
DR Ensembl; ENSECAT00000009456.3; ENSECAP00000007201.1; ENSECAG00000008971.3.
DR GeneID; 100059777; -.
DR KEGG; ecb:100059777; -.
DR CTD; 55214; -.
DR VGNC; VGNC:21114; P3H2.
DR GeneTree; ENSGT00940000159593; -.
DR HOGENOM; CLU_017820_0_0_1; -.
DR InParanoid; F6YCX7; -.
DR OMA; NEDTECR; -.
DR OrthoDB; 5398065at2759; -.
DR TreeFam; TF320837; -.
DR Proteomes; UP000002281; Chromosome 19.
DR Bgee; ENSECAG00000008971; Expressed in articular cartilage of joint and 22 other cell types or tissues.
DR ExpressionAtlas; F6YCX7; baseline.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0031418; F:L-ascorbic acid binding; IEA:InterPro.
DR GO; GO:0019797; F:procollagen-proline 3-dioxygenase activity; IBA:GO_Central.
DR GO; GO:0032963; P:collagen metabolic process; IBA:GO_Central.
DR Gene3D; 2.60.120.620; q2cbj1_9rhob like domain; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 2.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR039575; P3H.
DR InterPro; IPR006620; Pro_4_hyd_alph.
DR InterPro; IPR044862; Pro_4_hyd_alph_FE2OG_OXY.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR14049; LEPRECAN 1; 1.
DR PANTHER; PTHR14049:SF1; PROLYL 3-HYDROXYLASE 2; 1.
DR Pfam; PF13640; 2OG-FeII_Oxy_3; 1.
DR SMART; SM00702; P4Hc; 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..697
FT /note="procollagen-proline 3-dioxygenase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003345893"
FT DOMAIN 546..660
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
SQ SEQUENCE 697 AA; 79521 MW; 6F296068FF24D558 CRC64;
MRERIWAPLP LLLPLLLPPP LWGGPPALEP GPLQPFDLLY ASGVAAYYSG DYELAVRDLE
AALRSHRRLQ EIRARCARHC AARRPLAPPG AGPGAELPFF RAVLERARCY RSCESQRLGG
PASRHRVSED VRSDFQRRVP YNYLQRAYIK LNQLEKAVEA AHTFFMANPE HMEMQQNIEN
YRTMAGVEAL HLVDREAKPH LESYSAGVKH YEADDFELAI KYFEQALREY FSEDMECRAL
CEGPQRFEEY EYLGYKAGLY EAIADHYMQV LVCQHECVRE LATRPGRLSP IENFLPLHYD
YLQFAYYRVG EYVKALECAK AYLLLHPDDE DVLVNVDYYE SLLDDSVDPA SIEAREDLAM
FVKRHKLESE LIKSAAEGLG FSYTEPNYWI RYGGRQDENR VPSGVNVEGA EVHGLSMGKK
SPKIDRDLRE GGPLLYENIT FVYNSEQLNG TQRVLLDNVL SEEQCRELHS VASGIMLVGD
GYRGKTSPHT PNEKFEGATV LKALKFGYEG RVPLKSARLF YDISEKARKI VESYFMLNST
LYFSYTHMVC RTALSGQQDR RNDLSHPIHA DNCLLDPEAN ECWKEPPAYT FRDYSALLYM
NDDFEGGEFI FTEMDAKTVT ASIKPKCGRM ISFSSGGENP HGVKAVTKGQ RCAVALWFTL
DPLYRELERI QADEVIAILD QEQQGKHELS INPKDEL
//
