ID F6YVZ9_ORNAN Unreviewed; 1520 AA.
AC F6YVZ9;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 02-DEC-2020, sequence version 2.
DT 27-MAR-2024, entry version 67.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN Name=AGL {ECO:0000313|Ensembl:ENSOANP00000005070.2};
OS Ornithorhynchus anatinus (Duckbill platypus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Monotremata; Ornithorhynchidae; Ornithorhynchus.
OX NCBI_TaxID=9258 {ECO:0000313|Ensembl:ENSOANP00000005070.2, ECO:0000313|Proteomes:UP000002279};
RN [1] {ECO:0000313|Ensembl:ENSOANP00000005070.2, ECO:0000313|Proteomes:UP000002279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Glennie {ECO:0000313|Ensembl:ENSOANP00000005070.2,
RC ECO:0000313|Proteomes:UP000002279};
RX PubMed=18464734; DOI=10.1038/nature06936;
RA Warren W.C., Hillier L.W., Marshall Graves J.A., Birney E., Ponting C.P.,
RA Grutzner F., Belov K., Miller W., Clarke L., Chinwalla A.T., Yang S.P.,
RA Heger A., Locke D.P., Miethke P., Waters P.D., Veyrunes F., Fulton L.,
RA Fulton B., Graves T., Wallis J., Puente X.S., Lopez-Otin C., Ordonez G.R.,
RA Eichler E.E., Chen L., Cheng Z., Deakin J.E., Alsop A., Thompson K.,
RA Kirby P., Papenfuss A.T., Wakefield M.J., Olender T., Lancet D.,
RA Huttley G.A., Smit A.F., Pask A., Temple-Smith P., Batzer M.A.,
RA Walker J.A., Konkel M.K., Harris R.S., Whittington C.M., Wong E.S.,
RA Gemmell N.J., Buschiazzo E., Vargas Jentzsch I.M., Merkel A., Schmitz J.,
RA Zemann A., Churakov G., Kriegs J.O., Brosius J., Murchison E.P.,
RA Sachidanandam R., Smith C., Hannon G.J., Tsend-Ayush E., McMillan D.,
RA Attenborough R., Rens W., Ferguson-Smith M., Lefevre C.M., Sharp J.A.,
RA Nicholas K.R., Ray D.A., Kube M., Reinhardt R., Pringle T.H., Taylor J.,
RA Jones R.C., Nixon B., Dacheux J.L., Niwa H., Sekita Y., Huang X., Stark A.,
RA Kheradpour P., Kellis M., Flicek P., Chen Y., Webber C., Hardison R.,
RA Nelson J., Hallsworth-Pepin K., Delehaunty K., Markovic C., Minx P.,
RA Feng Y., Kremitzki C., Mitreva M., Glasscock J., Wylie T., Wohldmann P.,
RA Thiru P., Nhan M.N., Pohl C.S., Smith S.M., Hou S., Nefedov M.,
RA de Jong P.J., Renfree M.B., Mardis E.R., Wilson R.K.;
RT "Genome analysis of the platypus reveals unique signatures of evolution.";
RL Nature 453:175-183(2008).
RN [2] {ECO:0000313|Ensembl:ENSOANP00000005070.2}
RP IDENTIFICATION.
RC STRAIN=Glennie {ECO:0000313|Ensembl:ENSOANP00000005070.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR STRING; 9258.ENSOANP00000005070; -.
DR Ensembl; ENSOANT00000005071.3; ENSOANP00000005070.2; ENSOANG00000003192.3.
DR eggNOG; KOG3625; Eukaryota.
DR GeneTree; ENSGT00390000012596; -.
DR HOGENOM; CLU_001517_2_1_1; -.
DR OMA; HMREYTE; -.
DR TreeFam; TF300697; -.
DR Proteomes; UP000002279; Chromosome 4.
DR Bgee; ENSOANG00000003192; Expressed in heart and 8 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000002279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 31..115
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 121..552
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 698..975
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1074..1520
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
SQ SEQUENCE 1520 AA; 172897 MW; F1DD04EA9CA9D1D4 CRC64;
MGHSKQIRIL LLNDMEKLEK TLFRLDQGFE LQFRLGPTLQ GKEISVYTNY PSPGEPFNRQ
KFRLLAWENP TEKEDDSDKY CKLNLQQAGS FQYYFNQENE KSGGGYIVVD PILHVGADNQ
VLPLDCVTLQ TFLAKCLGPF DEWEDRLRVA KESGYNVIHF TPLQTLGLSR SCYSLADQLQ
LNPDFSRPNK KYTWNDVGQL VEKMRKEWNM LCITDVVYNH TAANSKWIQE HPECTYNLVN
SPHLKPAWIL DRALWHLTCD VAEGKYKEKG LPVLIENDQQ LNSIRKIVWE DIYPKIQLWE
FYQVDVNKAV QQFRVLLTQE NRKTVSKSDP KQLLKIIQDP EFKRLGCTVD MSIALSTFIP
HSNGPNAIDE CCNWFRKRIE ELNSEKLRIV NYHQEQAVNC LLGNVSYERL AGEGPKLGPV
TRKHPLVTRY FTFPYGEMTS VEEESLMHQP EKACFFMAHN GWVMGDDPLR NFAEPGSDVY
LRRELVCWGD SVKLRYGNKP EDCPYLWAHM KKYTEITAKY FQGVRLDNCH STPLHVAEYM
LDAARKLQPN LYVVAELFTG SEELDNIFVT RLGISSLIRE AMSAYNSHEE GRLVYRFGGE
PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSAYD ALPSSTIVSM ACCASGSTRG
YDELVPHQIS VVSEERFYTK WNPAATLSNP SEVNLQTGII AGRRAINKLH QELGAKGFIQ
VYVDQVDEDI VAVTRHCPSS HQSVVAVSRT AFRNPKTSAY SKEVPQMCIP GKIEEVVLEA
RTVERNVGSY VKDAKSINGM PDITVEIREH IQLNESKIVK QAGVATKGLH EYVQEIEFEN
LTPGSVIIFR VSLDPHAQVA VGILRNHLTQ FSPNFKIGSL PVDNSDPILK IPFASISSKL
TLADLNQLLY RCESEEQEDG GGCYDVPNWS PLKYGGLQGL MSVMAEIRPK NDLGHPFCNN
LRSGDWMIDY VSNRLISRSG SIAEVGKWFQ AMFFYLKQIP RYLIPCYFDA ILIGAYTTLL
DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG IGKFPSLPFL SPSLGDVPYR LNEITQEKEQ
CCVSLAAGLP HFSSGIFRCW GRDTFIALRG LLLITGRFLE ARNIILAFAG TLRHGLIPNL
LGQGTYARFN CRDAVWWWLQ CIQDYCKIVP KGTDILKCPV SRMYPSDDSS ALPAGTLDQP
LYEVIQEAMQ RHMQGIQFRE KNAGPQIDRN MKDEGFNVTA GVDDESGFVY GGNHFNCGTW
MDKMGESDRG RNRGIPATPR DGSAVEIVGL SKSTVRWLVE LSKKNVFPYH GVTVKRNEKE
VLITYDEWNR KIQDHFEKLF YVSEDPSDTN EKHPNLVHKR GIYKDSYGAS SPWCDYQLRP
NFTIAMVVAP ELFTPQKAWK ALEIAEKKLL GPLGMKTLDP DDMVYCGVYD NALDNDNYNL
AKGFNYHQGP EWLWPVGYFL RAKLYFSKLM GQETYTKTVF LIKNVLSRHY VHLERSPWKG
LPELTNENGQ YCSFSCETQA
//
