ID F6YYH0_ORNAN Unreviewed; 5978 AA.
AC F6YYH0;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 02-DEC-2020, sequence version 3.
DT 27-MAR-2024, entry version 87.
DE SubName: Full=Microtubule actin crosslinking factor 1 {ECO:0000313|Ensembl:ENSOANP00000015869.3};
GN Name=MACF1 {ECO:0000313|Ensembl:ENSOANP00000015869.3};
OS Ornithorhynchus anatinus (Duckbill platypus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Monotremata; Ornithorhynchidae; Ornithorhynchus.
OX NCBI_TaxID=9258 {ECO:0000313|Ensembl:ENSOANP00000015869.3, ECO:0000313|Proteomes:UP000002279};
RN [1] {ECO:0000313|Ensembl:ENSOANP00000015869.3}
RP IDENTIFICATION.
RC STRAIN=Glennie {ECO:0000313|Ensembl:ENSOANP00000015869.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000256|ARBA:ARBA00004245}.
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DR STRING; 9258.ENSOANP00000015869; -.
DR Ensembl; ENSOANT00000015872.3; ENSOANP00000015869.3; ENSOANG00000010005.4.
DR eggNOG; KOG0516; Eukaryota.
DR GeneTree; ENSGT00940000155824; -.
DR HOGENOM; CLU_000015_1_0_1; -.
DR TreeFam; TF335163; -.
DR Proteomes; UP000002279; Unplaced.
DR Bgee; ENSOANG00000010005; Expressed in fibroblast and 7 other cell types or tissues.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0045104; P:intermediate filament cytoskeleton organization; IEA:InterPro.
DR CDD; cd21240; CH_MACF1_rpt2; 1.
DR CDD; cd21188; CH_PLEC-like_rpt1; 1.
DR CDD; cd00051; EFh; 1.
DR CDD; cd00176; SPEC; 12.
DR Gene3D; 1.20.58.1060; -; 1.
DR Gene3D; 1.20.58.60; -; 29.
DR Gene3D; 1.10.418.10; Calponin-like domain; 2.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.30.920.20; Gas2-like domain; 1.
DR Gene3D; 3.90.1290.10; Plakin repeat; 2.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR041615; Desmoplakin_SH3.
DR InterPro; IPR041573; Desmoplakin_Spectrin-like.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR003108; GAR_dom.
DR InterPro; IPR036534; GAR_dom_sf.
DR InterPro; IPR049538; PCN-like_spectrin-like_rpt.
DR InterPro; IPR043197; Plakin.
DR InterPro; IPR035915; Plakin_repeat_sf.
DR InterPro; IPR001101; Plectin_repeat.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR23169; ENVOPLAKIN; 1.
DR PANTHER; PTHR23169:SF25; MICROTUBULE-ACTIN CROSS-LINKING FACTOR 1, ISOFORMS 1_2_3_4_5; 1.
DR Pfam; PF00307; CH; 2.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF02187; GAS2; 1.
DR Pfam; PF00681; Plectin; 4.
DR Pfam; PF17902; SH3_10; 1.
DR Pfam; PF00435; Spectrin; 14.
DR Pfam; PF18373; Spectrin_2; 1.
DR Pfam; PF21019; Spectrin_3; 1.
DR Pfam; PF21020; Spectrin_4; 1.
DR Pfam; PF21097; SR_plectin_7; 1.
DR SMART; SM00033; CH; 2.
DR SMART; SM01129; DELLA; 1.
DR SMART; SM00054; EFh; 2.
DR SMART; SM00243; GAS2; 1.
DR SMART; SM00250; PLEC; 9.
DR SMART; SM00150; SPEC; 30.
DR SUPFAM; SSF47576; Calponin-homology domain, CH-domain; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF143575; GAS2 domain-like; 1.
DR SUPFAM; SSF75399; Plakin repeat; 2.
DR SUPFAM; SSF46966; Spectrin repeat; 31.
DR PROSITE; PS00019; ACTININ_1; 1.
DR PROSITE; PS00020; ACTININ_2; 1.
DR PROSITE; PS50021; CH; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS51460; GAR; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Actin-binding {ECO:0000256|ARBA:ARBA00023203};
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Microtubule {ECO:0000256|ARBA:ARBA00022701};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000002279};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 18..130
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 143..247
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 853..910
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 5637..5672
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 5673..5708
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 5713..5791
FT /note="GAR"
FT /evidence="ECO:0000259|PROSITE:PS51460"
FT REGION 1829..1858
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1894..1934
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2311..2347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3009..3032
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5813..5870
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5894..5978
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 707..734
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1025..1093
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2193..2223
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2247..2302
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2806..2873
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3244..3271
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3487..3556
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3686..3760
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 5828..5847
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5901..5918
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5925..5948
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5959..5978
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5978 AA; 674726 MW; 4FC907C708E56E79 CRC64;
PGANPRPLCV FFLDERDRVQ KKTFTKWVNK HLMKVRKHIN DLYEDLRDGH NLISLLEVLS
GIKLPAGLKT LRLPREKGRM RFHRLQNVQI ALDFLKQRQV KLVNIRNDDI TDGNPKLTLG
LIWTIILHFQ ISDIYISGES GDMSAKEKLL LWTQKVTAGY TGIKCTNFSS CWSDGKMFNA
LIHRYRPDLV DMERVQIQSN RENLEQAFEV AERLGVTRLL DAEDVDVPSP DEKSVITYVS
SIYDAFPKVP EGGEGISATE VDSRWQEYQN RVDSLISWIK QHTILMSDKS FPQNPVELKA
LYNQYIHFKE TEIPAKEREK GRIEELYKLL EVWIEFGRIQ LPQGHHPNDV EEEWGKLILE
MLEREKSLRP AVERLELLLQ IANKIQNGAL SCEEKLTLAR NTLQADTAHL ELGQSVQYES
EVVPYLQEGE GLIRQLQVDL QILRDENYYQ EEDVLPTEGG SPWVGLSSGG RVWRLIPPPP
PSLTPLPPPA HPRILRLQDE LVTLRLECTN LYRKGHFTTL ELTQPSALTT APSKAEPLTK
GTHTTSASWF RKPMTRAELV AISSSEDEGN LRFVYELLAW VEEMQMKLER AEWGSDLPSV
ESQLETQQHI HTSVEELGSS VQEARMYEGK MSQNFRTSYA ETLGKLETQY CKLMETSGSR
LRHLESLHRF VSRATAELIW LNEKEEEELA YDWSDNNPNV TAKRNYFSEL RMELEEKQDV
FRSLQDTAEL LSSENHPAKQ TVEAYSAAVQ SQWQWIRQLC LCVEQHVKEN TAYFQFFSDA
RDLEAFLRNL QDSIKRKYSC DHNTSLLRLE DLLQDSMDEK EQLIQSKSSI ASLVGRSKTI
VQLKPRNPDH MLKNTISVKA ICDYRQIEIT ICKNDECVLE DNSHRTKWKV ISPTGNEAMV
PSVCFLIPPP NKEAVEMANR VEQSYQKVMT LWHQLHVNTK SLISWNYLRK DLDLVRSLPP
GECHQTMKSL RAHYEDFLQD SRDSELFSVA DRLRLEKEVD GCKEHFQRLL ESVENGREGA
DETVAKNYIS ELKNIRLHLE DCERRVVGRI KSPSGSWADG DALQDAALRV AEQERTQEDL
QQLKSELDDV SSRCSGFLQR SPSGPSAPTL RSELNLGSSI LQPFLSPRRL KTVDVIVRSA
QAAELLVKGY EIKLSQEEAV PADLSALQTH RSTLQQWLSD VKDKSSVFST LDEELAKAKV
VGEQLCRLTP ERSLDLERYQ EKGAQLRERW QRVGVQMETR SSDLESIQEV LRDYRACHGA
LIQWIEETTA QQEVMKPGQA EDSRVLSEQL SQQTVGSEIS KPPCRFFSPQ DYELQLMTYR
AFVESQQKSP GKRRRMISSS DAITQEFMDL RTRYTALVTL TTQHVKYISD ALRRLEEEEV
STVRALAEEL AMKKEQISDA IKTAQIFLAK HVTLFFPLSL GLGLDFLRCL SSFQGSGTVA
GVIDLGTVEI FPIFRAMQKG LIDQDTGLVL LEAQVITAGL VVPETNETLS LEAGLARNII
DQQTLQSLRE LRDAFSEMNT SAQGPLFLPV VAAVGEGRIS ENIGLKILET HLATGGFLLT
PGGDRMSLDE ALHRGLIPAP LHSKLQSRLM SRKNLIDPNT AEKISLADLT RRCVVHGDTG
LRLLPVKQLA GGTVSLKSGG KVSLFRAVQE GLIDRQVTVR LLEAQLLAGG IVDPRSGHRL
TAEEAVRQNL IDQDMACAIL VRQVQRGGVI DPVTGQRLTI DEAVGKDLVS SRRALVILES
LWSFMGLLWP ESGEVLPLAN ALEQGLLSTE LVWEILGNRR SIRALYRPPS PGVLTWKEAV
EAGVLDRNAV DRLKAIRFPD AIPSLQLADS PAGAKPGIGA PTATPPSGED QDGGFPKSED
EKLMFHLMTH SYINAHDGHR LLLVDEELYH ALTPSRKGES GTPGLEMCET GRRRPRAPED
GKGPAQVRSP GPCDDVTLRS LSSLETVRGP SERFGSGVHS PFFCQALDGE LSDLSALVSR
ELEAVSQIVA GQPQEVPAQL LKALEKDAKN LQKSFSSVSD TLSSRLLILR SVAEAERVSN
ALPSPPRLTE GVSASLWVVS VVSRGRGVGG PASFTPPFSC PLSPQDLKEP LSERKSQLDT
LAFDIQFFIS EHAQDLTPQQ SRQLLRLLNE LQRSSRELWE RASAQSGALH ARLQEVEREA
QVKVRLAVCH EKLEGLCHWL GEAERTLVDR QRRAASKEDL SALQQKQSDL EDLQKDVQDH
AGAFADAIKA TEEFLEENRT KLNPSELASL REKLRQAKEQ YRALRERTQT AQKELDGEVT
AAVRQETEKT KAANELEENQ RKIDSLLDWV SSLESPGGPS KLSLPTAGQT PGGRPGRGSQ
DTVDGQGGVD RAEEALDQQY ESLKARHQEL LSQQQNFILA TQSAQAFLDQ HGHNLSPEEG
SRLRRRLAEL KERYSASLAR SETELKQVQT LRDELRKFLR DHGEFETWLE QSEKELEGMR
REDGDLASLL RPMLLRQGSF SEDVISHKGD LRFITMSGQK VLDTEGGSGP SAAGVLVKDR
LEDATRRYTA LHSKCSKLGS HLNTLLDRYQ QFQGSTDGLQ AWMQQCEAEV ERLLADTVAS
DPKILQQQLA STKVNRAPDP LPGDAQSPLA ADPWHRGSPE ANHMCAFSSL SNPLLLPPPG
STDSILSRFQ SLSRRMAERS DLLQKAIAQS QSVQEGLESL LQSIAKVEGG LEGEQVASMS
SAAIQEALKL KQDIARQKSS LEATREMVTR FTETVDGATA AGLRGQLAEA SERFDRLCRR
QREKEAALKE LLPKVEQFER LTEKLRQFTD SRARSLASGN QPDRDIAHFS QQIQELNLEM
REQQENLDAL EHLASELSSC GISTDLSQHQ ETVQKLKKDF TELQKTARER EKETSSCQEQ
LDEFRKLVWG LRKWLKECEG SVPSAEATVG TQELEKQIEH VEVSGSGSGA GLEASRRAGA
VIFQTEGNLT EIQCELSDVN QQYESLGGVL RGRRDSLLAV LSRMREVGEE ARSALKWLES
TERNLEALDA TSSPTKTETV RAQAEHNKVP CPRSREQARL SINRSKVEGG QVVDQNEGVN
LWPLSPADSR WARANEVTVA RQQKLEKSAN QLDSFQAAEA QLRPWLKEKE LMMSVLGPLS
IDPNMLNAQK QQVQFMLKEF EARRQQHEQL NQAAQGIFAG PGDASPSTGQ ARDQLEGINQ
KWVELTERLN SRSSQIDQAI VKSTQYQELL RGLAEQVKAV GQQLSGQSAI SAQPDAVKQQ
LEETSEIRSD LERLDHEIAE AQALCDQLSL LIGEQYLKDE LRKRLETVAL PLKEIKTLTD
RLNRLQTALA STQQFQQMFD ELRAWLDDKR NQQARAGPTS AKLERLQAQL QEQEEFQKSL
NQHSGSYEVI VAEGETLLLS VQPGEEKAAL QGQLAALRSH WAELGKHAAD RQARLKDCLQ
KAQKYRWYVE DLVPWIEDCK GKMSGLQVTL DPAQLEATLL RAKGLLSDVE KRRSLLELLN
GAADLLLDSA QADEDDVRDE KAALERTVDA VTEELRAKTG SLEEMAQRLR EFQESVQTVE
KKLDGARHQL ELLDAMGPQA CSNKNLEKLR AHQETLQALE PQVDYLKRFT RGLLEDAPDG
QGPSRLLHRA EAAERDFASV RHRVDEGCRA MESRLDGIGQ FHGRVREMFS QLADLDDQLD
GMGAVGRDAD SLQSQIEDVR LFSGRVRGLK ADVAAAREEC RRMLDEEGSP DLLGLKRELE
TLNKQCGKLM ERGGTRQEQL ETTLARVEDF YNRLKELNRM TTAAEESEAL QWVVGTEVDA
INQQLADFKM FQKEQVDPLQ LKLQQVNGLG QGLIQSAGKN CDVQGLEHDM EAINARWNTL
NKKVAQRIAQ LQEALLHCGK FQDALEPLLS WLTDTEDLIA NQKPPSAEYK VVKAQIQEQK
LLQRLLDDRK ATVEMIQAEG GRIAQAAEPA DQEKIVGQLE SLEGRWAGLL HKAAARQRQL
EEILVLAKEF HETTEPISDF LSVTEKKLAN SEPVGTQTAK IQQQIVRHKV RRFLDDRAAG
VQLAVGVGRS LSSLTGPTER GALAERLDAL QARYREVRER CGRRAALLGQ ALCDARLFGD
GEVAVLNWLA EVEDKLGSVS VKDYKRDVLQ KQHADQLALN EEIVNRKKNV DQAIKNGQAL
LKQTTGEEVL LIQEKLDGIK TRYADITATS SKALRTLEQA RQLATKFQAT HEELTGWMNT
VADELAASGG QSPTGEQIPQ FQQRQKELKK EVMEHRLVLD TVNEVSRALL ELVPWRAREG
LDKLVSEANE QYKAVSDTIS QRVDEIDAAI QRSQQYEQAA DAELAWVAET KRKLAALGPI
RLEQDQTTAQ LQVQKAFSID IIRHKDSMDE LFSQRDEIFG TCGEEQKSLV QQYEAVSQLN
SERYARLERA QVLVNQFWET HEELDPWIQE TQTLIAQLPP PAIDHEQLKQ QQEDMRQLRE
SIAEHKPHID KLLKIGPQLK ELNPEEGEMV QERYRAAEAV YARIKEEVRL RALALDEAVS
QSAQFHDKIE PMLETLENLS SRLRVPPLIP AEVEKIRECI GDNKNATVEL EKLQPSFGAL
KRRGEELIGR SQGPDKDLAA KEIQDKLDQM VFFWEDIKAR AEEREMKFLD VLELAEKFWF
DMAALLATIR DTQDIVHDLE SPGIDPSIIK QQVEAAETIK EETDGLHEEL EFIRILGADL
IFACGETEKP EVKKSIDEMN NAWENLSKMW KERLERLEEA MQAAVQYQDA LQSMFDWLDN
TVIKLCNMPP VGTDLNTVKE QLNEMKEFKT EVYQQQIEME KLNHQGELML KKATDETDRD
IIREPLTELK HLWENLGEKI TGRQHKLEGA LLALGQFQHA LEELMGWLTH TEELLDAQRP
INGDPKVIEV ELAKHHVLKN DVLAHQATVE TVNKAGNELL ESSAGDDASS LRTRLETMNQ
CWESVLQKTE EREQQLQSTL QQAQGFHSEI EDFLLELTRM ENQLSASKPT GGLPETAREQ
LDAHLELHSQ LKAKEELYGQ LLDKGRLMLL SRQDSGTGSK TEQSIALLEQ KWQAVSSKME
ERKSKLEEVL SLATEFQNSL QEFINWLTLA EQSLNVAASP SLILNTVLGQ IDEHKVFANE
VNAHRDQIIE LDQTGNQLKF LSQKQDVVLI KNLLVSVQSR WEKVVQRSVE RGRALDDARK
RAKQFHEAWK KLIDWLEDAE KLLDSELEIS NDPDKIKLQL SKHKEFQKTL GGKQPVYDTT
IRTGRALKEK ALLPDDTQKL DNLLGEVRDK WDTVCGKSVE RQHKLEEALL FSGQFMDALQ
ALVDWLYKVE PQLAEDQPVH GDLDLVMNLM DAHKVFQKEL GKRTGTVQVL KRSGRELIEN
SRDDTTWVKG QLQELSTRWD TVCKLSVSKQ TRLEQALKQA EEFRNAVHML LEWLSEAEQT
LRFRGALPDD TEALQSLIDI HKEFMKKVEE KRVDVNAAVG MGEVILAVCH PDCITNIKHW
LTVIRARFEE VLTWAKQHQQ RLEAALSELV ANAELLEELL AWIQWAETTL IQRDQEPTPQ
NIDQVKALIS EHQAFMEEMT RKQPDVDRVT KTYKRKASEP THAPFIEKSR SNRKFPASST
KNPRINQLSA RWQQVWLLAL ERQRKLNDAL DRLEELKEFA NFDFDVWRKK YMRWMNHKKS
RVMDFFRRID KDQDGKITRQ EFIDGILASK FPTTKLEMTA VADIFDRDGD GYIDYYEFVA
ALHPNKDAYR PTTDADKIED EVTRQVAQCK CAKRFQVEQI GENKYRFFLG NQFGDSQQLR
LVRILRSTVM VRVGGGWMAL DEFLVKNDPC RGKLTWNLGK KFILPEGVSQ GMTTVPVTGR
RSKRLHGSVP TRSSSSASQS NPQLHLNAVL PQPPLPLGPG MGLDSGLPSS GSLSLRKVYS
RLWPGWRNLV GREYPKKTAS RPTSRAGSRT GSRASSRRGS DASDFDLLET QSACSDTSES
STAGCQGSAR RGLTKPSKIP TMSKKTTTAT SKTPGPKR
//
