ID F6Z596_ORNAN Unreviewed; 1093 AA.
AC F6Z596;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 2.
DT 27-MAR-2024, entry version 71.
DE SubName: Full=Solute carrier family 12 member 4 {ECO:0000313|Ensembl:ENSOANP00000015048.2};
GN Name=SLC12A4 {ECO:0000313|Ensembl:ENSOANP00000015048.2};
OS Ornithorhynchus anatinus (Duckbill platypus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Monotremata; Ornithorhynchidae; Ornithorhynchus.
OX NCBI_TaxID=9258 {ECO:0000313|Ensembl:ENSOANP00000015048.2, ECO:0000313|Proteomes:UP000002279};
RN [1] {ECO:0000313|Ensembl:ENSOANP00000015048.2, ECO:0000313|Proteomes:UP000002279}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Glennie {ECO:0000313|Ensembl:ENSOANP00000015048.2,
RC ECO:0000313|Proteomes:UP000002279};
RX PubMed=18464734; DOI=10.1038/nature06936;
RA Warren W.C., Hillier L.W., Marshall Graves J.A., Birney E., Ponting C.P.,
RA Grutzner F., Belov K., Miller W., Clarke L., Chinwalla A.T., Yang S.P.,
RA Heger A., Locke D.P., Miethke P., Waters P.D., Veyrunes F., Fulton L.,
RA Fulton B., Graves T., Wallis J., Puente X.S., Lopez-Otin C., Ordonez G.R.,
RA Eichler E.E., Chen L., Cheng Z., Deakin J.E., Alsop A., Thompson K.,
RA Kirby P., Papenfuss A.T., Wakefield M.J., Olender T., Lancet D.,
RA Huttley G.A., Smit A.F., Pask A., Temple-Smith P., Batzer M.A.,
RA Walker J.A., Konkel M.K., Harris R.S., Whittington C.M., Wong E.S.,
RA Gemmell N.J., Buschiazzo E., Vargas Jentzsch I.M., Merkel A., Schmitz J.,
RA Zemann A., Churakov G., Kriegs J.O., Brosius J., Murchison E.P.,
RA Sachidanandam R., Smith C., Hannon G.J., Tsend-Ayush E., McMillan D.,
RA Attenborough R., Rens W., Ferguson-Smith M., Lefevre C.M., Sharp J.A.,
RA Nicholas K.R., Ray D.A., Kube M., Reinhardt R., Pringle T.H., Taylor J.,
RA Jones R.C., Nixon B., Dacheux J.L., Niwa H., Sekita Y., Huang X., Stark A.,
RA Kheradpour P., Kellis M., Flicek P., Chen Y., Webber C., Hardison R.,
RA Nelson J., Hallsworth-Pepin K., Delehaunty K., Markovic C., Minx P.,
RA Feng Y., Kremitzki C., Mitreva M., Glasscock J., Wylie T., Wohldmann P.,
RA Thiru P., Nhan M.N., Pohl C.S., Smith S.M., Hou S., Nefedov M.,
RA de Jong P.J., Renfree M.B., Mardis E.R., Wilson R.K.;
RT "Genome analysis of the platypus reveals unique signatures of evolution.";
RL Nature 453:175-183(2008).
RN [2] {ECO:0000313|Ensembl:ENSOANP00000015048.2}
RP IDENTIFICATION.
RC STRAIN=Glennie {ECO:0000313|Ensembl:ENSOANP00000015048.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(in) + K(+)(in) = chloride(out) + K(+)(out);
CC Xref=Rhea:RHEA:72427, ChEBI:CHEBI:17996, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034453};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR RefSeq; XP_001505237.1; XM_001505187.2.
DR AlphaFoldDB; F6Z596; -.
DR STRING; 9258.ENSOANP00000015048; -.
DR Ensembl; ENSOANT00000015051.3; ENSOANP00000015048.2; ENSOANG00000009470.3.
DR GeneID; 100073385; -.
DR KEGG; oaa:100073385; -.
DR CTD; 6560; -.
DR eggNOG; KOG2082; Eukaryota.
DR GeneTree; ENSGT00940000157672; -.
DR HOGENOM; CLU_001883_1_2_1; -.
DR OrthoDB; 5490251at2759; -.
DR TreeFam; TF313657; -.
DR Proteomes; UP000002279; Chromosome X1.
DR Bgee; ENSOANG00000009470; Expressed in adult mammalian kidney and 8 other cell types or tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR000622; KCC1.
DR InterPro; IPR000076; KCL_cotranspt.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF46; SOLUTE CARRIER FAMILY 12 MEMBER 4; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 2.
DR Pfam; PF03522; SLC12; 2.
DR PRINTS; PR01081; KCLTRNSPORT.
DR PRINTS; PR01082; KCLTRNSPORT1.
PE 3: Inferred from homology;
KW Chloride {ECO:0000256|ARBA:ARBA00023214};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Potassium {ECO:0000256|ARBA:ARBA00022958};
KW Potassium transport {ECO:0000256|ARBA:ARBA00022538};
KW Reference proteome {ECO:0000313|Proteomes:UP000002279};
KW Symport {ECO:0000256|ARBA:ARBA00022847};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022847}.
FT TRANSMEM 126..147
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 159..184
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 261..280
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 287..308
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 418..443
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 464..490
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 510..528
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 564..582
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 588..609
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 621..638
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 132..308
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 425..703
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 717..833
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 847..1093
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 28..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..48
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1093 AA; 121955 MW; 013F0A55180EF0AD CRC64;
MPHFTVVPVE APRRGDYDNL EGLSWVDYRD PSHGGDEDPY DSLGSDGHCN HKESSPFLSL
SEAAKGSDHH DRNLALFEEE LDTRPKVSSL LGKLVSYTNL TQGAKEHEEA DIMEGSKRKV
PKSPSMGTLM GVYLPCMQNI FGVILFLRLT WIVGTAGVLQ SFLIVLICCC CTLLTTISMS
AIATNGVVPA GGSYFMISRS LGPEFGGAVG LCFYLGTTFA AAMYILGAIE ILLTYIVPPA
AIFHPTGVHD ATSATLNNMR VYGTIFLTFM TLVVFVGVKY VNKFASLFLA CVIISILSIY
AGGIKSIFDP PIFPVCMLGN RTLSRDQFDI CAKTTIVDNE TVATKLWKLF CHTSNLTTET
CDEYFLFNNI TEISGIPGAA SGILKDNLWS NYIEKGTILE RPGLPSVDDY GQKSSLPLYV
FADITTSFTV LVGIFFPSVT GIMAGSNRSG DLKDAQKSIP VGTILAIVTT SLVYFSSVVL
FGACIEGVVL RDKYGDGVSK NLVVGTLSWP SPWVIVIGSF FSTCGAGLQS LTGAPRLLQA
IAKDNIIPFL RVFGHGKTNG EPTWALLLTA LIAELGILIA SLDMVAPILS MFFLMCYLFV
NLACAVQTLL RTPNWRPRFK YYHWTLSFLG MSICLALMFI SSWYYALVAM VIAGMIYKYI
EYQGAEKEWG DGIRGLSLSA ARYALLRLEE GPPHTKNWRP QLLVLLKLDE DLHVKYPRML
TFASQLKAGK GLTIIGSVIQ GNFLESYGEA QAAEQTIKNM IEIEKVKGFC QVVVASKVRE
GIIHLIQSCG LGGMKHNSVV LGWPYGWRQS EDPRAWKTFI DTVRCSTAAH LALLVPKNVS
FYPSNHERYN EGNIDVWWIV HDGGMLMLLP FLLKQHKVWR KCRLRIFTVA QMDDNSIQMK
KDLATFLYHL RIEAEVEVVE MHNSDISAYT YERTLMMEQR SQMLRQMRLT KTEREREAQL
IKDRHSIIRL ESLYSDEEED IDPGPENIHM TWTRDKYDAE KLNRSNTMET FRELISIKPN
QSNVRRMHTA VKLNEVIVNR SHDARLVLLN MPGPPKNTNG DENYMEFLEV LTEGLERVLL
VRGGGREVIT IYS
//
