ID F6ZY82_XENTR Unreviewed; 1145 AA.
AC F6ZY82;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 02-JUN-2021, sequence version 4.
DT 27-MAR-2024, entry version 70.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSXETP00000019274};
GN Name=slc12a2 {ECO:0000313|Ensembl:ENSXETP00000019274};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364 {ECO:0000313|Ensembl:ENSXETP00000019274};
RN [1] {ECO:0000313|Ensembl:ENSXETP00000019274}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nigerian {ECO:0000313|Ensembl:ENSXETP00000019274};
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [2] {ECO:0000313|Ensembl:ENSXETP00000019274}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (JUL-2011) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR AlphaFoldDB; F6ZY82; -.
DR Ensembl; ENSXETT00000019274; ENSXETP00000019274; ENSXETG00000030071.
DR eggNOG; KOG2083; Eukaryota.
DR HOGENOM; CLU_001883_0_0_1; -.
DR TreeFam; TF313191; -.
DR Bgee; ENSXETG00000030071; Expressed in neurula embryo and 13 other cell types or tissues.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 265..291
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 312..336
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 356..375
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 384..403
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 435..457
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 469..488
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 546..569
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 628..646
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 658..677
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 151..202
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 238..739
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 748..1145
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 910..929
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 69..84
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1145 AA; 125186 MW; BF26A6B5B543F221 CRC64;
MEQAGKPGSE IPEQGKSPSS SLNPQPSQSA FPSSQELQVD DALAGRPVGP TPSQSRFQVD
LVTEGSKEAA AQELQQQADG ITQRGRGGSG SEEAKGRFRV NFVDPASPGG SSPENAGVDG
GGGGVSFQNG DTVLSEGSLH SGGTGHHYHY DTHTNTYYLR TFGHNTIDAV PRIDHYRHTA
ADLGEKLLRP SLAELHDELD KEPFEDGFAN GEEGTPTGDA ATTVADSKGV VKFGWIKGVL
VRCMLNIWGV MLFIRLSWIV GQAGIGLTVV VISMATVVTT ITGLSTSAIA TNGFVRGGGA
YYLISRSLGP EFGGAIGLIF AFANAVAVAM YVVGFAETVV ELLKENGALM VDDINDIRII
GAITVVLLLG ISVAGMEWEA KAQIVLLVIL LLAIVDFFIG TVIPSEDKKP KGFFGYKADI
FSENFGPDFR EGETFFSVFA IFFPAATGIL AGANISGDLA DPQHAIPRGT LLAILITTVV
YMGVAVSVGS CVVRDASGDV NSTLSAAMTN CTTAACNLNY DFSSCGHECK YGLMNSFQVM
SMVSGFAPLI TAGIFSATLS SALASLVSAP KVFQALCKDN IYPGLQMFAK GYGKNNEPLR
GYLLTFIIAL GFILIAELNL IAPIISNFFL ASYALINFSV FHASLAKSPG WRPAFRYYNM
WVSLIGALLC CGVMFVINWW AALLTYVIVI GLYIYVTYKK PDVNWGSSTQ ALTYLNALQH
TIRLAGVEDH VKNFRPQCLV LIGAPNSRPA LLHLVHAFTK NVGLMICGHV HMGPRRQAMK
ELLTDQARYQ RWLIKNKTKA FYSPVHAEDL RDGAQYLMQA AGLGRMRPNT LVVGFKKNWS
QCDMREVETY INLFHDAFDF QYGVVVIRLK EGLDISHLQG QEELLSSQEK SPGTKDVVVN
VDYSKKAEAE SSRPFASDSS PMMRKDSKGQ GISLDTADQR LLDASLQFQK KQGKSTIDVW
WLFDDGGLTL LIPYLITSKK KWRDCKIRVF IGGKINRIDH DRRAMATLLS KFRIDFSDIM
VLGDINTKPK KENVAAFEEM IEPFRLHEDE KDQEVAEKMK EEEPWRITDN ELELYKTKTH
RQIRLNELLK EHSSTANAIV MSLPVARKSA VSSALYMAWI EALSKDLPPI LLVRGNHQSV
LTFYS
//
