ID F7DPU7_HORSE Unreviewed; 363 AA.
AC F7DPU7;
DT 27-JUL-2011, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 3.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=protein phosphatase methylesterase-1 {ECO:0000256|ARBA:ARBA00013111};
DE EC=3.1.1.89 {ECO:0000256|ARBA:ARBA00013111};
GN Name=PPME1 {ECO:0000313|VGNC:VGNC:21750};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000008781.3, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000008781.3, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000008781.3,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000008781.3}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000008781.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Demethylates proteins that have been reversibly
CC carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding
CC to PPP2CA displaces the manganese ion and inactivates the enzyme.
CC {ECO:0000256|ARBA:ARBA00024698}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester +
CC H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) +
CC methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-
CC COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790,
CC ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89;
CC Evidence={ECO:0000256|ARBA:ARBA00000906};
CC -!- SUBUNIT: Binds PPP2CA and PPP2CB. {ECO:0000256|ARBA:ARBA00011604}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily.
CC {ECO:0000256|ARBA:ARBA00008645}.
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DR AlphaFoldDB; F7DPU7; -.
DR STRING; 9796.ENSECAP00000008781; -.
DR PaxDb; 9796-ENSECAP00000008781; -.
DR Ensembl; ENSECAT00000011260.3; ENSECAP00000008781.3; ENSECAG00000010756.4.
DR VGNC; VGNC:21750; PPME1.
DR GeneTree; ENSGT00390000004396; -.
DR HOGENOM; CLU_024818_0_1_1; -.
DR InParanoid; F7DPU7; -.
DR TreeFam; TF314697; -.
DR Proteomes; UP000002281; Chromosome 7.
DR Bgee; ENSECAG00000010756; Expressed in retina and 23 other cell types or tissues.
DR ExpressionAtlas; F7DPU7; baseline.
DR GO; GO:0051723; F:protein methylesterase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR016812; PPase_methylesterase_euk.
DR PANTHER; PTHR14189:SF0; PROTEIN PHOSPHATASE METHYLESTERASE 1; 1.
DR PANTHER; PTHR14189; PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR PRINTS; PR00111; ABHYDROLASE.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002281}.
FT DOMAIN 96..211
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000259|Pfam:PF12697"
FT REGION 272..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..296
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 363 AA; 40919 MW; 461141BFCE0A49D8 CRC64;
MSSLGQRESF FFLLKKWSYW LHVSKTDLMS QKLLFKCLLS LEVCNFAYSL VLPYFSHQYY
YEVNDKNFSE QKLRYVIFNY SYTFRVYKSG SEGPVLLLLH GGGHSALSWA VFTAAIISRV
QCRIVALDLR GHGETKVKNS EDLSAETMAK DVGNVVEAMY GDLPPPIMLI GHSMGGAIAV
HTASSNLVPS LLGLCMIDVV EGTAMDALNS MQNFLRGRPK TFKSLENAIE WSVKSGQIRN
LESARVSMVG QVKQCEGITS PEGSKSIVEG IIEEEEEDEE GSESVNKRKK EDDMETKKDH
PYTWRIELAK TEKYWDGWFR GLSNLFLSCP IPKLLLLAVL EAGCLRSGYQ HGRVLVLIDW
IKI
//
