ID F7KM64_9FIRM Unreviewed; 1064 AA.
AC F7KM64;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=HMPREF0993_00057 {ECO:0000313|EMBL:EGN39864.1};
OS Lachnospiraceae bacterium 5_1_57FAA.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=658085 {ECO:0000313|EMBL:EGN39864.1, ECO:0000313|Proteomes:UP000005617};
RN [1] {ECO:0000313|EMBL:EGN39864.1, ECO:0000313|Proteomes:UP000005617}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5_1_57FAA {ECO:0000313|EMBL:EGN39864.1,
RC ECO:0000313|Proteomes:UP000005617};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Daigneault M., Strauss J.,
RA Allen-Vercoe E., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Brown A.,
RA Chapman S.B., Chen Z., Dunbar C., Freedman E., Gearin G., Gellesch M.,
RA Goldberg J., Griggs A., Gujja S., Heiman D., Howarth C., Larson L., Lui A.,
RA MacDonald P.J.P., Mehta T., Montmayeur A., Murphy C., Neiman D.,
RA Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P.,
RA Stolte C., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Lachnospiraceae bacterium 5_1_57FAA.";
RL Submitted (MAY-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGN39864.1}.
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DR EMBL; ACTR01000001; EGN39864.1; -; Genomic_DNA.
DR AlphaFoldDB; F7KM64; -.
DR PATRIC; fig|658085.3.peg.60; -.
DR HOGENOM; CLU_005571_0_0_9; -.
DR Proteomes; UP000005617; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd19067; PfuEndoQ-like; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000005617}.
FT DOMAIN 475..734
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 735..978
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 449..482
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 496..503
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1064 AA; 119873 MW; 7EE98A81156921F7 CRC64;
MYIADLHIHS RYSRATSKDC TPEHLDLGAR RKGIHLVGTG DFTHPAWREE LKEKLVPAED
GLYVLKEEYR IQDETCTDRI IPRFVITGEI SSIYKKNGRV RKVHSLILLP GLEEAEVISR
KLEAIGNIHS DGRPILGLDC RDLLEIVLEL CPEAIFVPAH IWTPHFSLFG AFSGFDTVEE
CFGDLSPHIH AMETGLSSDP PMNWRVSALD SYHLISNSDA HSPAKLGREA NLLDIELSYQ
GLYDAIQKGQ GLTGTIEFFP EEGKYHFDGH RKCNLCLSPL DTEKYQGICP VCGRKITIGV
SHRVEQLADR AEGYVRADAR PFESLVPLPE VIGASVGHSA ASARVQKEYQ NMLRRLGSEF
DILRTMPLEE IRSVSGYLIS EGIGRLRDGK VERIPGFDGE YGTIKLFAPS EIENTSGQMD
FFDLLGGKKE GDGKSAKAKD RVNAAIEDGI TSAKAKQPEE DGKEQSSGTK LPLSDRLNRE
QERAVRSTAR TVAVIAGPGT GKTKTLISHI LYLIENRKVK PGEITAVTFT NQAAFQMRER
LRNALGKKKQ IHTMQIGTFH AICLAFLKDQ GREFSILDEE GAQEIARGLI EEYRLKDTVK
KFLCRVSAWK AEGELPQEDT DAFLAYQKYL EDRQLLDFDD LLLETLKMIE EPQASAGWEK
RFSYLLADEF QDINPLQYRL LKAWNKKGRE LFVIGDPDQS IYGFRGADAR CFDRLVEEHE
STEVIRLVEN YRSTPQILNA ALAVISHNPG EDRRLAANRE EGDRARLVTA ESGMGEAIFI
VKEINRLAGG IGMLEAHELS GAVRTEGRVR GFDEIAILYR ANHQADLLEK CLKKEGIPYI
VAGRGSFLQE EKVRGSICFF RYLDNPMDIL AKEQCLRLLW NMDGNLAAEE VLERMAGKYR
PLYQKKKPQK FLELWMEELQ LEDNEAVKKL VGMGVFYKTM PEFMLAVQLG IESDIKRCGN
RNYASGAVTL MTLHGAKGLE FPVTFIYGAR KGLIPLESEK GTTDLEEERR LLYVGMTRAM
EELILTTSKE ASGFLEGMPP ELMERETAHK RRVEQCHQMS LFEL
//
