ID F8KTN6_HELBC Unreviewed; 288 AA.
AC F8KTN6;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 24-JAN-2024, entry version 53.
DE RecName: Full=Cytidine monophosphate-N-acetylneuraminic acid hydroxylase {ECO:0000256|ARBA:ARBA00015403};
DE EC=1.14.18.2 {ECO:0000256|ARBA:ARBA00011904};
DE AltName: Full=CMP-N-acetylneuraminate monooxygenase {ECO:0000256|ARBA:ARBA00033362};
DE AltName: Full=CMP-Neu5Ac hydroxylase {ECO:0000256|ARBA:ARBA00030460};
DE AltName: Full=CMP-NeuAc hydroxylase {ECO:0000256|ARBA:ARBA00029883};
GN OrderedLocusNames=HBZC1_12200 {ECO:0000313|EMBL:CCB80206.1};
OS Helicobacter bizzozeronii (strain CIII-1).
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1002804 {ECO:0000313|EMBL:CCB80206.1, ECO:0000313|Proteomes:UP000008387};
RN [1] {ECO:0000313|EMBL:CCB80206.1, ECO:0000313|Proteomes:UP000008387}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CIII-1 {ECO:0000313|EMBL:CCB80206.1,
RC ECO:0000313|Proteomes:UP000008387};
RX PubMed=21705603; DOI=10.1128/JB.05439-11;
RA Schott T., Rossi M., Hanninen M.L.;
RT "Genome sequence of Helicobacter bizzozeronii strain CIII-1, an isolate
RT from human gastric mucosa.";
RL J. Bacteriol. 193:4565-4566(2011).
CC -!- FUNCTION: Sialic acids are components of carbohydrate chains of
CC glycoconjugates and are involved in cell-cell recognition and cell-
CC pathogen interactions. Catalyzes the conversion of CMP-N-
CC acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative
CC CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly
CC expressed at the surface of many cells.
CC {ECO:0000256|ARBA:ARBA00003414}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CMP-N-acetyl-beta-neuraminate + 2 Fe(II)-[cytochrome b5] + 2
CC H(+) + O2 = CMP-N-glycoloyl-beta-neuraminate + 2 Fe(III)-[cytochrome
CC b5] + H2O; Xref=Rhea:RHEA:16145, Rhea:RHEA-COMP:10438, Rhea:RHEA-
CC COMP:10439, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:29033, ChEBI:CHEBI:29034, ChEBI:CHEBI:57812,
CC ChEBI:CHEBI:58376; EC=1.14.18.2;
CC Evidence={ECO:0000256|ARBA:ARBA00029339};
CC -!- PATHWAY: Amino-sugar metabolism; N-acetylneuraminate metabolism.
CC {ECO:0000256|ARBA:ARBA00005141}.
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DR EMBL; FR871757; CCB80206.1; -; Genomic_DNA.
DR RefSeq; WP_013890625.1; NC_015674.1.
DR AlphaFoldDB; F8KTN6; -.
DR STRING; 1002804.HBZC1_12200; -.
DR KEGG; hbi:HBZC1_12200; -.
DR eggNOG; COG2220; Bacteria.
DR HOGENOM; CLU_965658_0_0_7; -.
DR UniPathway; UPA00628; -.
DR Proteomes; UP000008387; Chromosome.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0030338; F:CMP-N-acetylneuraminate monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006054; P:N-acetylneuraminate metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.60.15.10; Ribonuclease Z/Hydroxyacylglutathione hydrolase-like; 1.
DR InterPro; IPR027033; Cnh.
DR InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR PANTHER; PTHR46522; CYTIDINE MONOPHOSPHATE-N-ACETYLNEURAMINIC ACID HYDROXYLASE; 1.
DR PANTHER; PTHR46522:SF1; CYTIDINE MONOPHOSPHATE-N-ACETYLNEURAMINIC ACID HYDROXYLASE; 1.
DR Pfam; PF13483; Lactamase_B_3; 1.
DR SUPFAM; SSF50022; ISP domain; 1.
DR SUPFAM; SSF56281; Metallo-hydrolase/oxidoreductase; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000008387}.
FT DOMAIN 48..106
FT /note="Rieske"
FT /evidence="ECO:0000259|PROSITE:PS51296"
SQ SEQUENCE 288 AA; 31972 MW; 787581D350349815 CRC64;
MEPKHLGRFS TQSIQTSLTL PSLQEGIYPL AEGAYFVKIV RGGSLEWVIN NTYDHMGNPL
CGGGNTLRCP KHGWVLDLNS LTYTNGACKR PLDPTSYTLD NHTLTLISAT SLTNPYLPTQ
KGGFICRYLN HACLYFESAG LKIITDPWLV GPAFLTGWWL ASPSSQEAMA CLQEADVIYI
SHNHPDHLGP ETFVHIPKDK FFICANFESQ STKKALKSLG FTRVIALDFG EIYAFNDRIS
CAVFKSGDFR DDSGLYLCLE GHEVLLSVDS NFLNRHTLPQ HLTLPTAP
//
