ID F8L7J8_SIMNZ Unreviewed; 275 AA.
AC F8L7J8;
DT 21-SEP-2011, integrated into UniProtKB/TrEMBL.
DT 21-SEP-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN Name=rluC {ECO:0000313|EMBL:CCB88731.1};
GN OrderedLocusNames=SNE_A08540 {ECO:0000313|EMBL:CCB88731.1};
OS Simkania negevensis (strain ATCC VR-1471 / Z).
OC Bacteria; Chlamydiota; Chlamydiia; Parachlamydiales; Simkaniaceae;
OC Simkania.
OX NCBI_TaxID=331113 {ECO:0000313|EMBL:CCB88731.1, ECO:0000313|Proteomes:UP000000496};
RN [1] {ECO:0000313|EMBL:CCB88731.1, ECO:0000313|Proteomes:UP000000496}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC VR-1471 / Z {ECO:0000313|Proteomes:UP000000496};
RX PubMed=21690563; DOI=10.1093/molbev/msr161;
RA Collingro A., Tischler P., Weinmaier T., Penz T., Heinz E., Brunham R.C.,
RA Read T.D., Bavoil P.M., Sachse K., Kahane S., Friedman M.G., Rattei T.,
RA Myers G.S., Horn M.;
RT "Unity in variety--the pan-genome of the Chlamydiae.";
RL Mol. Biol. Evol. 28:3253-3270(2011).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR EMBL; FR872582; CCB88731.1; -; Genomic_DNA.
DR RefSeq; WP_013943198.1; NC_015713.1.
DR AlphaFoldDB; F8L7J8; -.
DR STRING; 331113.SNE_A08540; -.
DR KEGG; sng:SNE_A08540; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_8_2_0; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000000496; Chromosome gsn.131.
DR GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028, ECO:0000313|EMBL:CCB88731.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000496};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 82..220
FT /note="Pseudouridine synthase RsuA/RluA-like"
FT /evidence="ECO:0000259|Pfam:PF00849"
FT ACT_SITE 113
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 275 AA; 31158 MW; 12D4A655A401A210 CRC64;
MKFSIGERDA GKTILSFLKE KLKVSGQAIK RGVDQGAVRL NGVVERFSSV RLQKGDLFEF
DQAQLKEKQE FSLPILFEEE NFLICSKPSG LVTDQEVFKR LLGRSVFLVH RLDKDTSGVI
LVATIQKMQK KLEALFKSRK VRKVYVALVK GVMQQESGTI DNRLMKKKTY QGQAIWGSTK
NPKGLRAITH WKCIGKAPGK SLVQCEPETG RTHQLRVHLS EMGHPILGDH HYGREAKFPP
EIDRLCLHAY RLVFNHPDTG EKIQATAPIP KLFKI
//
