ID   F9FVA6_FUSOF            Unreviewed;       709 AA.
AC   F9FVA6;
DT   19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT   19-OCT-2011, sequence version 1.
DT   27-MAR-2024, entry version 50.
DE   RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000256|ARBA:ARBA00039099};
DE            EC=2.1.1.216 {ECO:0000256|ARBA:ARBA00039099};
GN   ORFNames=FOXB_10337 {ECO:0000313|EMBL:EGU79177.1};
OS   Fusarium oxysporum (strain Fo5176) (Fusarium vascular wilt).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium oxysporum species complex.
OX   NCBI_TaxID=660025 {ECO:0000313|EMBL:EGU79177.1};
RN   [1] {ECO:0000313|EMBL:EGU79177.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fo5176 {ECO:0000313|EMBL:EGU79177.1};
RX   PubMed=21942452; DOI=10.1094/mpmi-08-11-0212;
RA   Thatcher L.F., Gardiner D.M., Kazan K., Manners J.;
RT   "A highly conserved effector in Fusarium oxysporum is required for full
RT   virulence on Arabidopsis.";
RL   Mol. Plant Microbe Interact. 25:180-190(2012).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Trm1 family. {ECO:0000256|PROSITE-ProRule:PRU00958}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EGU79177.1}.
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DR   EMBL; AFQF01002710; EGU79177.1; -; Genomic_DNA.
DR   AlphaFoldDB; F9FVA6; -.
DR   STRING; 660025.F9FVA6; -.
DR   PaxDb; 5507-FOXG_08363P0; -.
DR   GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR   Gene3D; 3.30.56.70; N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002905; Trm1.
DR   InterPro; IPR042296; tRNA_met_Trm1_C.
DR   NCBIfam; TIGR00308; TRM1; 1.
DR   PANTHER; PTHR10631; N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR10631:SF3; TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; 1.
DR   Pfam; PF02005; TRM; 2.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51626; SAM_MT_TRM1; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU00958};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU00958};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU00958};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU00958}; tRNA processing {ECO:0000256|PROSITE-ProRule:PRU00958};
KW   tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|PROSITE-
KW   ProRule:PRU00958}.
FT   REGION          138..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          619..663
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        138..162
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        635..649
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   709 AA;  78774 MW;  A78594B571E0A223 CRC64;
     MQILSKQHSY MFRVFWRSRS NFGAGAILDL TNRFSKTRTL STNRNYRALC DKPIAHRQFS
     TTAKMAEEKV NVETVTVEGK EFRTVTEGKA TILVPQGAKI GEDRGEVQQV FYNPIQQYNR
     DLSVLAIKTY GEMSLERRKE QYESRMNKQG KKRKRGDDEQ KPTEAVNPPP QDKPQATEEA
     AKPNDAEAQQ PKKEIKPSFR ILDALSASGL RALRYAHELP FVTSVKANDL SDTAAESIRM
     NAKHNGLEDK ITVTQGDALA LMYRGIADDL SNRDKGGNPG KANKFDVIDL DPYGTAAPFF
     DAAVQSIRDD GGLLCITCTD SAVWAGHSYC EKTFALYGGI PIKGMHSHEA GLRLVLNAVA
     TSAARYGLAI EPLLSLSIDF YTKFFIKVTK SPQSVKFLAS KTMLVYSCDS GCGAWETQPM
     LRSKPAPNKK GSGAFYKHTM AQGPSADRHC EHCGMKMHIN GPMYGGHIHS QEFIERLLAQ
     IPKADPSIYG TMPRLEGMLR TALEEYLPGP EVKEPVDPKD AQLATVDHYP FFIIPSRLAN
     VVSCVTPSED MVRGALIHLG YRTARSHCRP GSIKTDAPWS TIWWIITEWI RQKSPIKESS
     IKKNSAAWKI LTDAGIIGQE KPEAAEETAK DGSAMEGVEQ QTSEAPVEGT AGATNGDDKP
     VPSEMELRKT LVFDENLARL ARRRGEQKLV RYQMNPRENW GPLAKASRR
//