ID F9MPP5_9FIRM Unreviewed; 1095 AA.
AC F9MPP5;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 24-JAN-2024, entry version 54.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:EGS33116.1};
GN ORFNames=HMPREF1040_0699 {ECO:0000313|EMBL:EGS33116.1};
OS Megasphaera sp. UPII 135-E.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Megasphaera.
OX NCBI_TaxID=1000569 {ECO:0000313|EMBL:EGS33116.1, ECO:0000313|Proteomes:UP000004137};
RN [1] {ECO:0000313|EMBL:EGS33116.1, ECO:0000313|Proteomes:UP000004137}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UPII 135-E {ECO:0000313|EMBL:EGS33116.1,
RC ECO:0000313|Proteomes:UP000004137};
RA Harkins D.M., Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA Nelson K.E.;
RL Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGS33116.1}.
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DR EMBL; AFUG01000032; EGS33116.1; -; Genomic_DNA.
DR RefSeq; WP_007392799.1; NZ_AFUG01000032.1.
DR AlphaFoldDB; F9MPP5; -.
DR eggNOG; COG1197; Bacteria.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000004137; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000004137}.
FT DOMAIN 564..725
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 746..900
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1095 AA; 125793 MW; 878551F026A79B55 CRC64;
MKQLFKWLDQ EENLRQIKNF FINGGCNHIH GVGGSAKHIL VAQGIDTALG VTLLITSSSE
EMEKWKSDLQ FLVPTIPIFT FPFVVQELFS TAAKSMERVA RQMEVLAQLR EDKKCIILTT
VEEASQYIIA PSQLDNRVIS FEVKQEYERA ALLEKLIQNG YERVELVERC GHFSIRGDIL
DIYAVNQTKP IRLEFFGDTI EKIRLFDVLS QTSIQTVEQV QVLPFSLSWD EHQPYNATIL
DYLHKGQIIW DEPNRLRDNL KKALTESDDY VSHLCPWRTW VTRTYPCSQV LISLLSPTFY
DMKVESSIHL VTKRMSSFQK QFSLLKEELL HWHAEGKTVV LVIDNLNRKA SLQAWMQQEG
FSIETHIPDT LQKGVCYLVT GNLQNGFEFP HAHLVILCER DIYGMQKRRL FLKHTQSQKI
NTFTDLKKGD YVVHHSHGIG RYMGLKTMEI DDVHRDYLEI HYAGNDILYV PIEQMALVQR
YIGNEGDIPK LHRMGGKDWQ KARAKAQKSV DNLAEKLLSL YAKREVVSGF AYPPDTPFQR
EFEEAFPYEE TEDQLRAVAE IKKYMERPFP MDCLVCGDVG FGKTEVAMRA IFKAVMSHKQ
VAVLVPTTVL AQQHFLTFTE RLASFGVQCE VLNRFRSVKE RKNILARTKL GQVDVLIGTH
SILNKQVQFK QLGLLVVDEE QRFGVAQKEK WKSLAAHVDV LSLSATPIPR TLHMSLVKLR
EMCIMETPPI DRFPVQTYVT EYDPTVIREA ITREKRRGGQ VFFIYNQVES MGYMKNELER
LVPEFSIAMA HGQMSGAMLE AVMFDFYEGK YDILLCSSLV ENGLDVANAN TIIVYDADHF
GLSQLYQMRG RVGRSHNLAY AYLCYRKNKV LSEIAEKRLS AIKEFTELGS GFKIAMRDLE
IRGAGNLLGR EQHGNIAGVG FSMYCQMLEG AIKRLQNGIS DETERIETVL DIQVNAYIDN
EYITNNGQKI DIYQRLAIAD TSDEINALEK ELTDRYGTMT DPVIALLQVA IIRVAARKLG
ITLISQKKDY VEIKWQQVAQ MPHLSALDPA LQKRVRYIRG LPTVWRIDWM QEMDIISFIN
YLIKVFCLQR KGTKK
//
