GenomeNet

Database: UniProt
Entry: F9VEN7
LinkDB: F9VEN7
Original site: F9VEN7 
ID   QUEG_LACGL              Reviewed;         351 AA.
AC   F9VEN7;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 2.
DT   16-JAN-2019, entry version 35.
DE   RecName: Full=Epoxyqueuosine reductase {ECO:0000255|HAMAP-Rule:MF_00916};
DE            EC=1.17.99.6 {ECO:0000255|HAMAP-Rule:MF_00916};
DE   AltName: Full=Queuosine biosynthesis protein QueG {ECO:0000255|HAMAP-Rule:MF_00916};
GN   Name=queG {ECO:0000255|HAMAP-Rule:MF_00916};
GN   OrderedLocusNames=LCGL_1328;
OS   Lactococcus garvieae (strain Lg2) (Enterococcus seriolicida).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=420890;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Lg2;
RX   PubMed=21829716; DOI=10.1371/journal.pone.0023184;
RA   Morita H., Toh H., Oshima K., Yoshizaki M., Kawanishi M., Nakaya K.,
RA   Suzuki T., Miyauchi E., Ishii Y., Tanabe S., Murakami M., Hattori M.;
RT   "Complete genome sequence and comparative analysis of the fish
RT   pathogen Lactococcus garvieae.";
RL   PLoS ONE 6:E23184-E23184(2011).
CC   -!- FUNCTION: Catalyzes the conversion of epoxyqueuosine (oQ) to
CC       queuosine (Q), which is a hypermodified base found in the wobble
CC       positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
CC       {ECO:0000255|HAMAP-Rule:MF_00916}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + epoxyqueuosine(34) in tRNA = A + H2O +
CC         queuosine(34) in tRNA; Xref=Rhea:RHEA:32159, Rhea:RHEA-
CC         COMP:10345, Rhea:RHEA-COMP:10346, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:17499, ChEBI:CHEBI:82831,
CC         ChEBI:CHEBI:82834; EC=1.17.99.6; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00916};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00916}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00916}.
CC   -!- SIMILARITY: Belongs to the QueG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00916}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAK60788.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
DR   EMBL; AP009333; BAK60788.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_016170890.1; NC_017490.1.
DR   SMR; F9VEN7; -.
DR   STRING; 420890.LCGL_1328; -.
DR   EnsemblBacteria; BAK60788; BAK60788; LCGL_1328.
DR   GeneID; 34203144; -.
DR   KEGG; lgv:LCGL_1328; -.
DR   PATRIC; fig|420890.5.peg.1316; -.
DR   eggNOG; ENOG4105EH2; Bacteria.
DR   eggNOG; COG1600; LUCA.
DR   KO; K18979; -.
DR   OrthoDB; 1478472at2; -.
DR   BioCyc; LGAR420890:G1H60-1314-MONOMER; -.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000008520; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0052693; F:epoxyqueuosine reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00916; QueG; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR013542; DUF1730.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   InterPro; IPR004453; QueG.
DR   PANTHER; PTHR30002; PTHR30002; 1.
DR   Pfam; PF08331; DUF1730; 1.
DR   SUPFAM; SSF46548; SSF46548; 1.
DR   TIGRFAMs; TIGR00276; TIGR00276; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;
KW   Metal-binding; Oxidoreductase; Queuosine biosynthesis;
KW   Reference proteome; tRNA processing.
FT   CHAIN         1    351       Epoxyqueuosine reductase.
FT                                /FTId=PRO_0000416073.
FT   DOMAIN      177    205       4Fe-4S ferredoxin-type.
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       185    185       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       188    188       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       191    191       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
FT   METAL       195    195       Iron-sulfur 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00916}.
FT   METAL       237    237       Iron-sulfur 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00916}.
FT   METAL       240    240       Iron-sulfur 2. {ECO:0000255|HAMAP-
FT                                Rule:MF_00916}.
FT   METAL       244    244       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00916}.
SQ   SEQUENCE   351 AA;  39622 MW;  E422C65083850542 CRC64;
     MKSLKEEIQK LAADLNIQKI GFTKADDFEY LRASLIEQKE AGHTSGFEHK NLDERLQPKL
     SLEDAKTIIS IGIAYPNKAE EKPEKTEYRR GSFSRGSWGE DYHHVLRRKL KELAEGIEKI
     AGDFNYTAMV DTGALVDVAV AARAGLGFVG KNGLLVSKEY GSWMFLGELV TNLDIEEDQP
     VDYGCGSCTR CVDFCPTKAL LGDGRLNAQR CLSYQTQTRG VMPEEYREKI KTVIYGCDIC
     QVVCPYNKGI NSHFHPEMEP DPDLTNPELL PLLDLSNKEF ANKFGNMAGS WRGKNPIQRN
     VVYALGNAND RSALPKLHDI AENDVRDYMV DAAEWAIEKL ENKHRMRPRK R
//
DBGET integrated database retrieval system