ID   G0ED22_PYRF1            Unreviewed;       417 AA.
AC   G0ED22;
DT   19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT   19-OCT-2011, sequence version 1.
DT   27-MAR-2024, entry version 48.
DE   RecName: Full=cysteine desulfurase {ECO:0000256|ARBA:ARBA00012239};
DE            EC=2.8.1.7 {ECO:0000256|ARBA:ARBA00012239};
GN   OrderedLocusNames=Pyrfu_0712 {ECO:0000313|EMBL:AEM38581.1};
OS   Pyrolobus fumarii (strain DSM 11204 / 1A).
OC   Archaea; Thermoproteota; Thermoprotei; Desulfurococcales; Pyrodictiaceae;
OC   Pyrolobus.
OX   NCBI_TaxID=694429 {ECO:0000313|EMBL:AEM38581.1, ECO:0000313|Proteomes:UP000001037};
RN   [1] {ECO:0000313|EMBL:AEM38581.1, ECO:0000313|Proteomes:UP000001037}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 11204 / 1A {ECO:0000313|Proteomes:UP000001037};
RX   PubMed=21886865;
RA   Anderson I., Goker M., Nolan M., Lucas S., Hammon N., Deshpande S.,
RA   Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S., Huntemann M.,
RA   Liolios K., Ivanova N., Pagani I., Mavromatis K., Ovchinikova G., Pati A.,
RA   Chen A., Palaniappan K., Land M., Hauser L., Brambilla E.M., Huber H.,
RA   Yasawong M., Rohde M., Spring S., Abt B., Sikorski J., Wirth R.,
RA   Detter J.C., Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P., Lapidus A.;
RT   "Complete genome sequence of the hyperthermophilic chemolithoautotroph
RT   Pyrolobus fumarii type strain (1A).";
RL   Stand. Genomic Sci. 4:381-392(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-
CC         alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA-
CC         COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972,
CC         ChEBI:CHEBI:64428; EC=2.8.1.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00001357};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933};
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. Csd subfamily.
CC       {ECO:0000256|ARBA:ARBA00010447}.
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DR   EMBL; CP002838; AEM38581.1; -; Genomic_DNA.
DR   RefSeq; WP_014026258.1; NC_015931.1.
DR   AlphaFoldDB; G0ED22; -.
DR   STRING; 694429.Pyrfu_0712; -.
DR   GeneID; 11139180; -.
DR   KEGG; pfm:Pyrfu_0712; -.
DR   eggNOG; arCOG00065; Archaea.
DR   HOGENOM; CLU_003433_2_5_2; -.
DR   InParanoid; G0ED22; -.
DR   Proteomes; UP000001037; Chromosome.
DR   GO; GO:0031071; F:cysteine desulfurase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0006534; P:cysteine metabolic process; IEA:InterPro.
DR   CDD; cd06453; SufS_like; 1.
DR   Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR010970; Cys_dSase_SufS.
DR   InterPro; IPR016454; Cysteine_dSase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   NCBIfam; TIGR01979; sufS; 1.
DR   PANTHER; PTHR43586; CYSTEINE DESULFURASE; 1.
DR   PANTHER; PTHR43586:SF8; CYSTEINE DESULFURASE-RELATED; 1.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   PIRSF; PIRSF005572; NifS; 2.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE   3: Inferred from homology;
KW   Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001037}.
FT   DOMAIN          29..403
FT                   /note="Aminotransferase class V"
FT                   /evidence="ECO:0000259|Pfam:PF00266"
SQ   SEQUENCE   417 AA;  46578 MW;  178AFE6977A52977 CRC64;
     MRFLPGEGFL LDYESIREDF PILSKKRIVY LDNAATTQKP RQVIEAVRRF YEESNANILR
     GIYELSMKAT MLYEEAHIVV AKFIGARDWR EVVFTKNSTD SINMIAYALI GRIKSGDNIV
     VTEMEHHSNF LPWVRLARLT GAELRVAPID EHGRLDMEKL ESLIDEKTRV VAIVHASNVL
     GVVNDVRRVA RIAHQYDALV VVDAAQSVPH MPVNVRELEA DFLVFSGHKM LGPTGIGVLW
     GRLDILEELE PPVYGGGAVK SVRVESGEVK VEYAELPWKW EPGTPNIAGA VGLAEAVRYL
     QGIGLEKIEA HERTLTRMLI KMLGELDVRM VGDIPAEERV GIVSFTLDGF KPLFVALRLG
     RMKIAVRAGF HCAEPLHRKL GFNEGSVRAS MYLYNGPDDI EKLVDALRGI LRGEGGS
//