ID G0NUL9_CAEBE Unreviewed; 300 AA.
AC G0NUL9;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=tRNA methyltransferase 10 homolog A {ECO:0000256|ARBA:ARBA00014673};
DE EC=2.1.1.221 {ECO:0000256|ARBA:ARBA00012797};
GN ORFNames=CAEBREN_09987 {ECO:0000313|EMBL:EGT37900.1};
OS Caenorhabditis brenneri (Nematode worm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=135651 {ECO:0000313|Proteomes:UP000008068};
RN [1] {ECO:0000313|Proteomes:UP000008068}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PB2801 {ECO:0000313|Proteomes:UP000008068};
RG Caenorhabditis brenneri Sequencing and Analysis Consortium;
RA Wilson R.K.;
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00000855};
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DR EMBL; GL379951; EGT37900.1; -; Genomic_DNA.
DR AlphaFoldDB; G0NUL9; -.
DR STRING; 135651.G0NUL9; -.
DR EnsemblMetazoa; CBN09987.1; CBN09987.1; WBGene00148712.
DR eggNOG; KOG2967; Eukaryota.
DR HOGENOM; CLU_034384_7_0_1; -.
DR InParanoid; G0NUL9; -.
DR OMA; FKKNDGW; -.
DR Proteomes; UP000008068; Unassembled WGS sequence.
DR GO; GO:0052905; F:tRNA (guanosine(9)-N1)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd18101; Trm10euk_A; 1.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR016653; TRM10/TRM10A.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; TRNA (GUANINE-9-) METHYLTRANSFERASE; 1.
DR PANTHER; PTHR13563:SF13; TRNA METHYLTRANSFERASE 10 HOMOLOG A; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PIRSF; PIRSF016323; tRNA_m1G_mtfrase_met; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000008068};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 86..277
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000259|PROSITE:PS51675"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 276..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 31..48
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..293
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 208
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR016323-1"
FT BINDING 184
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PIRSR:PIRSR016323-2"
FT BINDING 204
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PIRSR:PIRSR016323-2"
FT BINDING 216
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PIRSR:PIRSR016323-2"
FT BINDING 230
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PIRSR:PIRSR016323-2"
SQ SEQUENCE 300 AA; 35064 MW; 920BBD789D310F40 CRC64;
MSQDTPEQIE ESVEVSNNKF QINTPEDNQD ESRQPVLEGM SKKQLRRQKY KQKWEEKKLI
KRAAERIRKK EKRAALKETG DLSKLRKRKD FRTMAQSNSK QRIALDMSFD DLMIEKDQKR
TVQQIGWCYT ANRHSPNPFQ FHVVGFNGPS RKIYDGNEHN LNQDIFLHHD KLETVFEPKD
IIYLSSESDN VLSELDDTKV YVIGGIVDHN SQKGLCHRIA GEKGFGHARL PLDEHILMKS
RRVLTINQVY EILVHYSVHK DWKAALLSII PERKNAQVKE EVRTEEPEQN KSESEEQLVS
//
