ID G0S7R0_CHATD Unreviewed; 1461 AA.
AC G0S7R0;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=5'-3' exoribonuclease 1 {ECO:0000256|PIRNR:PIRNR006743};
DE EC=3.1.13.- {ECO:0000256|PIRNR:PIRNR006743};
GN ORFNames=CTHT_0028500 {ECO:0000313|EMBL:EGS21010.1};
OS Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
OS (Thermochaetoides thermophila).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Chaetomiaceae; Thermochaetoides.
OX NCBI_TaxID=759272 {ECO:0000313|Proteomes:UP000008066};
RN [1] {ECO:0000313|EMBL:EGS21010.1, ECO:0000313|Proteomes:UP000008066}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1495 / CBS 144.50 / IMI 039719
RC {ECO:0000313|Proteomes:UP000008066};
RX PubMed=21784248; DOI=10.1016/j.cell.2011.06.039;
RA Amlacher S., Sarges P., Flemming D., van Noort V., Kunze R., Devos D.P.,
RA Arumugam M., Bork P., Hurt E.;
RT "Insight into structure and assembly of the nuclear pore complex by
RT utilizing the genome of a eukaryotic thermophile.";
RL Cell 146:277-289(2011).
CC -!- FUNCTION: Multifunctional protein that exhibits several independent
CC functions at different levels of the cellular processes. 5'-3'
CC exonuclease component of the nonsense-mediated mRNA decay (NMD) which
CC is a highly conserved mRNA degradation pathway, an RNA surveillance
CC system whose role is to identify and rid cells of mRNA with premature
CC termination codons and thus prevents accumulation of potentially
CC harmful truncated proteins. {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR006743}.
CC -!- SIMILARITY: Belongs to the 5'-3' exonuclease family.
CC {ECO:0000256|PIRNR:PIRNR006743}.
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DR EMBL; GL988041; EGS21010.1; -; Genomic_DNA.
DR RefSeq; XP_006693306.1; XM_006693243.1.
DR STRING; 759272.G0S7R0; -.
DR GeneID; 18256888; -.
DR KEGG; cthr:CTHT_0028500; -.
DR eggNOG; KOG2045; Eukaryota.
DR HOGENOM; CLU_001581_1_2_1; -.
DR OMA; VASWPWF; -.
DR OrthoDB; 167745at2759; -.
DR Proteomes; UP000008066; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004534; F:5'-3' RNA exonuclease activity; IEA:UniProt.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR CDD; cd18673; PIN_XRN1-2-like; 1.
DR Gene3D; 1.25.40.1050; -; 1.
DR Gene3D; 2.170.260.40; -; 1.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 2.30.30.750; -; 1.
DR Gene3D; 3.40.50.12390; -; 2.
DR InterPro; IPR027073; 5_3_exoribonuclease.
DR InterPro; IPR016494; 5_3_exoribonuclease_1.
DR InterPro; IPR014722; Rib_uL2_dom2.
DR InterPro; IPR041385; SH3_12.
DR InterPro; IPR040992; XRN1_D1.
DR InterPro; IPR047007; XRN1_D1_sf.
DR InterPro; IPR041106; XRN1_D2_D3.
DR InterPro; IPR041412; Xrn1_helical.
DR InterPro; IPR004859; Xrn1_N.
DR InterPro; IPR047008; XRN1_SH3_sf.
DR PANTHER; PTHR12341:SF83; 5'-3' EXORIBONUCLEASE 1; 1.
DR PANTHER; PTHR12341; 5'->3' EXORIBONUCLEASE; 1.
DR Pfam; PF18129; SH3_12; 1.
DR Pfam; PF18332; XRN1_D1; 1.
DR Pfam; PF18334; XRN1_D2_D3; 1.
DR Pfam; PF17846; XRN_M; 1.
DR Pfam; PF03159; XRN_N; 1.
DR PIRSF; PIRSF006743; Exonuclease_Xnr1; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR006743};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|PIRNR:PIRNR006743};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR006743};
KW Nonsense-mediated mRNA decay {ECO:0000256|PIRNR:PIRNR006743};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|PIRNR:PIRNR006743};
KW Reference proteome {ECO:0000313|Proteomes:UP000008066};
KW RNA-binding {ECO:0000256|PIRNR:PIRNR006743}.
FT DOMAIN 1..227
FT /note="Xrn1 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03159"
FT DOMAIN 275..669
FT /note="Xrn1 helical"
FT /evidence="ECO:0000259|Pfam:PF17846"
FT DOMAIN 718..906
FT /note="5'-3' exoribonuclease 1 D1"
FT /evidence="ECO:0000259|Pfam:PF18332"
FT DOMAIN 910..1136
FT /note="Exoribonuclease Xrn1 D2/D3"
FT /evidence="ECO:0000259|Pfam:PF18334"
FT DOMAIN 1154..1224
FT /note="5'-3' exoribonuclease 1 SH3-like"
FT /evidence="ECO:0000259|Pfam:PF18129"
FT REGION 1262..1461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1279..1293
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1317..1335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1395..1409
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1443..1461
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1461 AA; 163834 MW; 1DC0D3E70FFB648A CRC64;
MGVPKFFRWL SERYPAISQL IAENRIPEFD CLYLDMNGII HNCTHKDVDD VHFRLTEEEM
FIAIFNYIEH LFGKIKPKKL FFMAVDGVAP RAKMNQQRAR RFRTALDAER AREKALREGK
ELPKEEPFDS NCITPGTEFM AKLSLQLKYF INKKVSEDRD WQGPEIVLSG HEVPGEGEHK
IMEYIRNARA QPDYNPNTRH CLYGLDADLI MLGLLSHDPH FCLLREEVTF GRQAKHKSKE
LEHQNFYLLH LCIVREYLEL EFQELKEPGA IPFPFDMERV IDDFILMAFF VGNDFLPNLP
RLHINEGALA LMFKIYKKVL PKCDGYINEN GKVNLDRLKI LLEELEQEEY RFFEQEHEDT
KWLQGKKMLE NNEAEQAKAK AKGKLIISPS QKALWKQKIR KWLLNSTSPT LDLGADLNAA
DRKFVEDLAE AAHIEWATKP DDDGIRHLVL SRPVMDEDDE EDEEAHAALY RIAKRYDNAQ
VLDLSSEEAK AAVQEKYKQK FQEWKDKYYL SKMDGWTPQN LEQELKKMAE NYVQGLQWVL
YYYYRGIASW PWFYGYHYAP MISDVVKGLG ADLNFKLGQP FRPNEQLMGV LPDRSKKIVP
KVYWDLMTDP NSPIIDFYPR EFELDLNGKK MDWEAVVKIP FIDEKRLLEA MAPRNALLTE
EEKARNEFGV PLKFTYSEDV DFVYPSSLVD VFPPIFHCHC VENIFDLPPM EGLEYRAGLA
EGALLNVEAL AGFPTLATLP YTASLAFHGV NVFQQESRNE SMIVRLTDSH LRTKIDTAKV
KLGQRCFVGY PFLQEAKVVA VSDELFEYTL ASDGSGQVIE RHHTPREIEE WKKKAERIEN
FYSKRLGILI GPVESLVTVH MLKGLRKTDE GATVKEYGEV PGMETDYAAQ IIVDEVVNED
ERFIEQAALP IEEEFPVKSN GFFVGDYNYG QPLEVVGYTK DGKLSIWLAV PTAREPEFAK
QIIHEAHRSQ LYQPSYMVAK QLGLHPLTLS KITSSYYVRT VGDLRVNLGL NLKFESKKQK
VLGYSSKSAT GWEFSQAAVL LISEYMRRFP DFFAAIVKNP SGSELNETDL FPDPAYATAR
VKEIGAWLKT LKTSSMERVP LDAEQLDSDV VMRLAAKADE LGLGNQQTVS KKMNGVPRNA
VIRPEDAEHR LGNQVFSLGD RVVYVARKGK VPIAFRGTVV GISRTPTNKL IDVVFDVTFL
SGTTLGDRCP PFRGQTVPSN VLLNLTNRQL VVETKAGQLR RAPINPSVTT LTAHGGYMLN
GKKYRDAPAP PPLQAPYRSV VNGSANSRGG ANTPRGGSRG GGRNGSPAHA NLPHRPAPQH
QNHQPQQSHP QQQINGHHAP GGARGGRGGP AGTLPFRGGR GGGAPRAGFA TPQRGGAGFL
PGNVGAGIPH PASAPPMYSA VPPPPSLDAP ARGSGRGRGR GGVARGGNRG GRGRGGVSRG
SVPAAQAASQ PPQVAASASQ G
//
