ID G1BRE4_9CAUD Unreviewed; 612 AA.
AC G1BRE4;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE SubName: Full=DNA polymerase {ECO:0000313|EMBL:AEK07410.1};
GN Name=45 {ECO:0000313|EMBL:AEK07410.1};
GN ORFNames=ROCKSTAR_45 {ECO:0000313|EMBL:AEK07410.1};
OS Mycobacterium phage Rockstar.
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Veracruzvirus; Veracruzvirus rockstar.
OX NCBI_TaxID=1034144 {ECO:0000313|EMBL:AEK07410.1, ECO:0000313|Proteomes:UP000221175};
RN [1] {ECO:0000313|EMBL:AEK07410.1, ECO:0000313|Proteomes:UP000221175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Rockstar {ECO:0000313|Proteomes:UP000221175};
RX PubMed=22282335; DOI=10.1128/JVI.06870-11;
RG the Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science Program;
RG the KwaZulu-Natal Research Institute for Tuberculosis and HIV Mycobacterial Genetics Course Students;
RG the Phage Hunters Integrating Research and Education Program;
RA Hatfull G.F.;
RT "Complete Genome Sequences of 138 Mycobacteriophages.";
RL J. Virol. 86:2382-2384(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
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DR EMBL; JF704111; AEK07410.1; -; Genomic_DNA.
DR OrthoDB; 14842at10239; -.
DR Proteomes; UP000221175; Genome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00023109};
KW Viral DNA replication {ECO:0000256|ARBA:ARBA00023109}.
FT DOMAIN 15..212
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 375..572
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 612 AA; 68323 MW; 638CD79C54D7264C CRC64;
MIEHRHEVAG DEVVIRVVET EDDLEGFRDF IRAHLGFLGL DSETTGLDIY SDDFRCRTVQ
FGTPNEGWVV PVELGRPFEG AVIEALESVN GFVLHNAAFD LQVFEKTLGV PMETMWPKVK
DTRILSHLVD PRGKDEGGIG HSLEETVRHY VDSEVADKVK TLMADLAAAR KGVTKATIWK
KVELFDPVYN LYAGMDPILA ARLIQKLAPL VKVRDELIDN EHRLAEICSY MERQGFLLDV
EYTEELSLDL KVKESHYNEV ALNFGCEKIN STDQVADVLE SMGVRIIGRT PSGKRQVNDD
LLSKLVAEGS PEVSQFAEAV IEGKKAGKWR KTWVDTFLKT RDSQNRCHAS INPLRARTAR
MSITGIPAQT LPSGDWIIRR CFLADEGHKM ASVDYQAQEL RVLAALSRDK AMVEAFANDE
DLHLKTARAA WPDREITKDS PERKYAKTVN FGRVYGGGAK TVAEQTGLDM AQAQQVVSGF
DRAYPEVQKL SQRLQREAIR NGYITTPFID GLGGRRLPVD PQRAYSALNY LIQSSSRDVT
ARALLRLHDA GFTPYLRLPI HDEILASVPA EHAEWGAKRI GELMAEQMGP VLIGTDPEVG
GRSWGSLYGA DY
//
