ID G1KKM1_ANOCA Unreviewed; 381 AA.
AC G1KKM1;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 2.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Proton-coupled zinc antiporter SLC30A8 {ECO:0000256|ARBA:ARBA00040844};
DE AltName: Full=Solute carrier family 30 member 8 {ECO:0000256|ARBA:ARBA00033403};
DE AltName: Full=Zinc transporter 8 {ECO:0000256|ARBA:ARBA00042037};
GN Name=slc30a8 {ECO:0000313|Ensembl:ENSACAP00000010676.3};
OS Anolis carolinensis (Green anole) (American chameleon).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Iguania; Dactyloidae; Anolis.
OX NCBI_TaxID=28377 {ECO:0000313|Ensembl:ENSACAP00000010676.3, ECO:0000313|Proteomes:UP000001646};
RN [1] {ECO:0000313|Ensembl:ENSACAP00000010676.3, ECO:0000313|Proteomes:UP000001646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JBL SC #1 {ECO:0000313|Ensembl:ENSACAP00000010676.3,
RC ECO:0000313|Proteomes:UP000001646};
RG The Genome Sequencing Platform;
RA Di Palma F., Alfoldi J., Heiman D., Young S., Grabherr M., Johnson J.,
RA Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Anolis carolinensis (Green Anole Lizard).";
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACAP00000010676.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Proton-coupled zinc ion antiporter mediating the entry of
CC zinc into the lumen of pancreatic beta cell secretory granules, thereby
CC regulating insulin secretion. {ECO:0000256|ARBA:ARBA00037214}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+)(out) + Zn(2+)(in) = 2 H(+)(in) + Zn(2+)(out);
CC Xref=Rhea:RHEA:72627, ChEBI:CHEBI:15378, ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00034428};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}.
CC Cytoplasmic vesicle, secretory vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004638}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004638}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily.
CC {ECO:0000256|ARBA:ARBA00008873}.
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DR RefSeq; XP_003219509.1; XM_003219461.2.
DR AlphaFoldDB; G1KKM1; -.
DR STRING; 28377.ENSACAP00000010676; -.
DR Ensembl; ENSACAT00000010897.4; ENSACAP00000010676.3; ENSACAG00000010841.4.
DR GeneID; 100553837; -.
DR KEGG; acs:100553837; -.
DR CTD; 169026; -.
DR eggNOG; KOG1482; Eukaryota.
DR GeneTree; ENSGT00940000160706; -.
DR HOGENOM; CLU_013430_0_1_1; -.
DR InParanoid; G1KKM1; -.
DR OrthoDB; 1331349at2759; -.
DR TreeFam; TF313382; -.
DR Proteomes; UP000001646; Chromosome 4.
DR Bgee; ENSACAG00000010841; Expressed in dewlap and 2 other cell types or tissues.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0030073; P:insulin secretion; IBA:GO_Central.
DR GO; GO:0009749; P:response to glucose; IBA:GO_Central.
DR GO; GO:0010043; P:response to zinc ion; IBA:GO_Central.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; Cation efflux protein transmembrane domain; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR NCBIfam; TIGR01297; CDF; 1.
DR PANTHER; PTHR11562; CATION EFFLUX PROTEIN/ ZINC TRANSPORTER; 1.
DR PANTHER; PTHR11562:SF37; ZINC TRANSPORTER 8; 1.
DR Pfam; PF01545; Cation_efflux; 1.
DR SUPFAM; SSF160240; Cation efflux protein cytoplasmic domain-like; 1.
DR SUPFAM; SSF161111; Cation efflux protein transmembrane domain-like; 1.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|ARBA:ARBA00022449};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000001646};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 103..122
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 143..162
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 174..195
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 230..253
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 265..288
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 22..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 381 AA; 42303 MW; 7F2D9C35AA1991C7 CRC64;
MASKKSLERA CLVNDRDTKR YSLTLSSNGS NSDQENHHEE PVGTGIHAVH HCHSYSKGYE
RRKREQHEAR LRLCVATVIC IIIMVAEIIG GHVAGSLAVI TDAAHVLVDL MSFLISILSL
WLSSKSPSKR LTYGWHRAEI LGALFSMIII WVVTGVLTYL AILRLLHPQY EIEATVMLIT
SGCAVIANII LSLMLHHKGG GNLYNVHQSS DGRSDPERTL ASSNASVRAA FIHAVGDLFQ
SISVLISALI IFLKPEYKMA DPICTFVFSL LVLGTTCAML RDILLVLMEG MPKGMSYKAV
KERILTVDKV ESVHSLHVWS LTMNQPVLSA HIVTDTANKQ QILNEITELL IDTYNFYTVT
LQIEPKADQQ PDCVFCQDPR D
//
