ID G1KKM9_ANOCA Unreviewed; 666 AA.
AC G1KKM9;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 3.
DT 27-MAR-2024, entry version 65.
DE SubName: Full=AlkB homolog 8, tRNA methyltransferase {ECO:0000313|Ensembl:ENSACAP00000010712.4};
GN Name=ALKBH8 {ECO:0000313|Ensembl:ENSACAP00000010712.4};
OS Anolis carolinensis (Green anole) (American chameleon).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Iguania; Dactyloidae; Anolis.
OX NCBI_TaxID=28377 {ECO:0000313|Ensembl:ENSACAP00000010712.4, ECO:0000313|Proteomes:UP000001646};
RN [1] {ECO:0000313|Ensembl:ENSACAP00000010712.4, ECO:0000313|Proteomes:UP000001646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JBL SC #1 {ECO:0000313|Ensembl:ENSACAP00000010712.4,
RC ECO:0000313|Proteomes:UP000001646};
RG The Genome Sequencing Platform;
RA Di Palma F., Alfoldi J., Heiman D., Young S., Grabherr M., Johnson J.,
RA Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Anolis carolinensis (Green Anole Lizard).";
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACAP00000010712.4}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|ARBA:ARBA00001954};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR RefSeq; XP_003219355.1; XM_003219307.3.
DR RefSeq; XP_008106231.1; XM_008108024.1.
DR RefSeq; XP_008106232.1; XM_008108025.2.
DR AlphaFoldDB; G1KKM9; -.
DR Ensembl; ENSACAT00000010933.4; ENSACAP00000010712.4; ENSACAG00000010946.4.
DR GeneID; 100555791; -.
DR KEGG; acs:100555791; -.
DR CTD; 91801; -.
DR GeneTree; ENSGT00940000158563; -.
DR HOGENOM; CLU_029501_4_0_1; -.
DR InParanoid; G1KKM9; -.
DR TreeFam; TF316056; -.
DR Proteomes; UP000001646; Chromosome 3.
DR Bgee; ENSACAG00000010946; Expressed in hindlimb bud and 13 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005506; F:iron ion binding; IEA:Ensembl.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:InterPro.
DR GO; GO:0106335; F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:Ensembl.
DR GO; GO:0008270; F:zinc ion binding; IEA:Ensembl.
DR GO; GO:0006974; P:DNA damage response; IEA:Ensembl.
DR GO; GO:0030488; P:tRNA methylation; IEA:Ensembl.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:Ensembl.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd12431; RRM_ALKBH8; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR034256; ALKBH8_RRM.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR13069:SF21; ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8; 1.
DR PANTHER; PTHR13069; UNCHARACTERIZED; 1.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR Pfam; PF08241; Methyltransf_11; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000001646};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transferase {ECO:0000256|ARBA:ARBA00022603};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 223..340
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 541..564
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 666 AA; 74763 MW; 560D1A8574EE8CA0 CRC64;
MEISGINQQS QKLSKKEKKL RQKQMKVRHT LLRHEGIECV SYPTKSLVIA NGGLGNGVSR
CQVLKLAERC GLVEALLMPP NKPYSFLKYE MLEDSKRAYD SLNGEDVASE DNTQNVVLYF
NFVEKVPWED MTPSTLPPGL RVIEEVVSPE EEGQLLECID WGKDEVIPNA QKSLKHRRVK
HFGYEFQYDN NNVDRDRPLP GGLPDICNTL LAKWLKMGYI KESSDQLTIN QYEPGQGIPP
HIDTHSAFED EIISLSLGAG IVMDFKHPDG QAAAVMLPQR SLLVMSGESR YLWTHGITPR
KFDIVQASQE QKLGTVTADI GDLTLNKRGT RTSFTFRKVR RGPCKCSYPS VCDSQCKVTA
PSFPDSESEA SKLEKEYVHD VYEQIAQHFS STRHSPWPRV VEFLRALPRG AIVADVGCGN
GKYLGVSKDM YVFGCDRSEA LVNICGEKKF QAFVCDALSV PIRSGSCDAC ISIAVIHHFS
TAERRLATLS ELVRLLRPGG RALVYVWALE QEYNKQKSKY LKGKRAGNSN MDETISNAIE
EAGDSESQDS AQPDGGVSPP KDRYCSANEA VSTRLPVHTN RTSFDSQDLL VPWHLKDGTK
KKMGHTKLCQ PVTSSENSQK CSPVFHRYYH VFCEGELELL CKRLNCVNVQ ESYYDQGNWC
VILEKK
//
