ID G1LVE7_AILME Unreviewed; 5372 AA.
AC G1LVE7;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 02-JUN-2021, sequence version 2.
DT 27-MAR-2024, entry version 83.
DE SubName: Full=Microtubule actin crosslinking factor 1 {ECO:0000313|Ensembl:ENSAMEP00000011048.2};
GN Name=MACF1 {ECO:0000313|Ensembl:ENSAMEP00000011048.2};
OS Ailuropoda melanoleuca (Giant panda).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Ursidae; Ailuropoda.
OX NCBI_TaxID=9646 {ECO:0000313|Ensembl:ENSAMEP00000011048.2, ECO:0000313|Proteomes:UP000008912};
RN [1] {ECO:0000313|Ensembl:ENSAMEP00000011048.2, ECO:0000313|Proteomes:UP000008912}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20010809; DOI=10.1038/nature08696;
RA Li R., Fan W., Tian G., Zhu H., He L., Cai J., Huang Q., Cai Q., Li B.,
RA Bai Y., Zhang Z., Zhang Y., Wang W., Li J., Wei F., Li H., Jian M., Li J.,
RA Zhang Z., Nielsen R., Li D., Gu W., Yang Z., Xuan Z., Ryder O.A.,
RA Leung F.C., Zhou Y., Cao J., Sun X., Fu Y., Fang X., Guo X., Wang B.,
RA Hou R., Shen F., Mu B., Ni P., Lin R., Qian W., Wang G., Yu C., Nie W.,
RA Wang J., Wu Z., Liang H., Min J., Wu Q., Cheng S., Ruan J., Wang M.,
RA Shi Z., Wen M., Liu B., Ren X., Zheng H., Dong D., Cook K., Shan G.,
RA Zhang H., Kosiol C., Xie X., Lu Z., Zheng H., Li Y., Steiner C.C.,
RA Lam T.T., Lin S., Zhang Q., Li G., Tian J., Gong T., Liu H., Zhang D.,
RA Fang L., Ye C., Zhang J., Hu W., Xu A., Ren Y., Zhang G., Bruford M.W.,
RA Li Q., Ma L., Guo Y., An N., Hu Y., Zheng Y., Shi Y., Li Z., Liu Q.,
RA Chen Y., Zhao J., Qu N., Zhao S., Tian F., Wang X., Wang H., Xu L., Liu X.,
RA Vinar T., Wang Y., Lam T.W., Yiu S.M., Liu S., Zhang H., Li D., Huang Y.,
RA Wang X., Yang G., Jiang Z., Wang J., Qin N., Li L., Li J., Bolund L.,
RA Kristiansen K., Wong G.K., Olson M., Zhang X., Li S., Yang H., Wang J.,
RA Wang J.;
RT "The sequence and de novo assembly of the giant panda genome.";
RL Nature 463:311-317(2010).
RN [2] {ECO:0000313|Ensembl:ENSAMEP00000011048.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000256|ARBA:ARBA00004245}.
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DR STRING; 9646.ENSAMEP00000011048; -.
DR Ensembl; ENSAMET00000011521.2; ENSAMEP00000011048.2; ENSAMEG00000010358.2.
DR eggNOG; KOG0516; Eukaryota.
DR GeneTree; ENSGT00940000155824; -.
DR HOGENOM; CLU_000015_0_1_1; -.
DR TreeFam; TF335163; -.
DR Proteomes; UP000008912; Unassembled WGS sequence.
DR GO; GO:0042995; C:cell projection; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0045104; P:intermediate filament cytoskeleton organization; IEA:InterPro.
DR CDD; cd21240; CH_MACF1_rpt2; 1.
DR CDD; cd21188; CH_PLEC-like_rpt1; 1.
DR CDD; cd00051; EFh; 1.
DR CDD; cd00176; SPEC; 17.
DR Gene3D; 1.20.58.1060; -; 1.
DR Gene3D; 1.20.58.60; -; 34.
DR Gene3D; 1.10.418.10; Calponin-like domain; 2.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 3.30.920.20; Gas2-like domain; 1.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR041615; Desmoplakin_SH3.
DR InterPro; IPR041573; Desmoplakin_Spectrin-like.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR003108; GAR_dom.
DR InterPro; IPR036534; GAR_dom_sf.
DR InterPro; IPR049538; PCN-like_spectrin-like_rpt.
DR InterPro; IPR043197; Plakin.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR23169; ENVOPLAKIN; 1.
DR PANTHER; PTHR23169:SF25; MICROTUBULE-ACTIN CROSS-LINKING FACTOR 1, ISOFORMS 1_2_3_4_5; 1.
DR Pfam; PF00307; CH; 2.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF02187; GAS2; 1.
DR Pfam; PF17902; SH3_10; 1.
DR Pfam; PF00435; Spectrin; 19.
DR Pfam; PF18373; Spectrin_2; 1.
DR Pfam; PF21019; Spectrin_3; 1.
DR Pfam; PF21020; Spectrin_4; 1.
DR Pfam; PF21097; SR_plectin_7; 1.
DR SMART; SM00033; CH; 2.
DR SMART; SM01129; DELLA; 1.
DR SMART; SM00054; EFh; 2.
DR SMART; SM00243; GAS2; 1.
DR SMART; SM00150; SPEC; 34.
DR SUPFAM; SSF47576; Calponin-homology domain, CH-domain; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF143575; GAS2 domain-like; 1.
DR SUPFAM; SSF46966; Spectrin repeat; 31.
DR PROSITE; PS00019; ACTININ_1; 1.
DR PROSITE; PS00020; ACTININ_2; 1.
DR PROSITE; PS50021; CH; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS51460; GAR; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Actin-binding {ECO:0000256|ARBA:ARBA00023203};
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Microtubule {ECO:0000256|ARBA:ARBA00022701};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000008912};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 27..130
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 143..247
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 817..874
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT DOMAIN 5025..5060
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 5061..5096
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 5101..5173
FT /note="GAR"
FT /evidence="ECO:0000259|PROSITE:PS51460"
FT REGION 4935..4965
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5189..5372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 426..478
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1352..1382
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1446..1498
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1578..1605
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1912..1939
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1989..2016
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2129..2210
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2558..2633
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2871..2912
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 3049..3095
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 4075..4102
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 5208..5287
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5296..5312
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5319..5372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5372 AA; 613111 MW; CFFE0A7AFE3F6E73 CRC64;
MWPSYQDEHE FNAHLVGRMC CMDERDRVQK KTFTKWVNKH LMKVRKHIND LYEDLRDGHN
LISLLEVLSG IKLPREKGRM RFHRLQNVQI ALDFLKQRQV KLVNIRNDDI TDGNPKLTLG
LIWTIILHFQ ISDIYISGES GDMSAKEKLL LWTQKVTAGY TGIKCTNFSS CWSDGKMFNA
LIHRYRPDLV DMERVQIQSN RENLEQAFEV AERLGVTRLL DAEDVDVPSP DEKSVITYVS
SIYDAFPKVP EGGEGISATE VDSRWQEYQS RVDSLIPWIK QHTILMSDKS FPQNPVELKA
LYNQYIHFKE TEILAKEREK GRIEELYKLL EVWIEFGRIK LPQGYHPNDV EEEWGKLIIE
MLEREKSLRP AVERLELLLQ IANKIQNGAL NCEEKLTLAK NTLQADAAHL ESGQPVQCES
DVIMYIQECE GLIRQLQVDL QILRDENYYQ LEELAFRVMR LQDELVTLRL ECTNLYRKGH
FSSLELAPPS TLTTTHLKAE PLTKGTHSSS TSWFRKPMTR AELVSISSSE DEGNLRFVYE
LLSWVEEMQM KLERAEWGND LPSVELQLET QQHIHTSVEE LGSSVKEARL YEGKMSQNFH
TSYVETLGKL ETQYCKLKEM SSFRMRHLQS LHKFVSRATA ELIWLNEKEE EELAYDWSDN
NPNISAKKNY FSELTMQLEE KQDVFRSLQD TAELLSLENH PAKQTVEAYS AAAQSQLQWM
KQLCLCVEQH VKENTAYFQF FSDARDLESF LRNLQDSIKR KYSCDHNTSL SRLEDLLQDS
MDEKEQLIQS KSSVASLVGR SKTIVQLKPR SPDHVLKSTI SVKAICDYRQ IEITICKNDE
CVLEDNSQRT KWKVISPTGN EAMVPSVCFL IPSPNKDAIE MATRVEQSYQ KVMALWHQLH
VNTKTLISWN YLRKDLDLVN TWNLEKLRSS APGECHQVMK NLQAHCEDFL QDSRDSLLFS
VADRLRLEEE VEACKTHFQH LMKSIENEDK EETVAKMYIS ELKNIQLRLE ECEQRLVKRI
QSPASSRTDK DARQENALRI AEQERTQEDL QQLRSEFDAV CTKCNSFLHQ SPSGSSVPTL
RSELNLLVEK MDCVYGLSTV YLNKLKTIDV IVRSIQDAEL LVKGYEIKLS QEEAVPADLS
ALESHRSTLR HWLSDVKDKN SVFSVLDEEI AKAKVVAEQL SRLTPERNLD LERYQEKGSQ
LQDRWHRVIV QLETRQSELE SIQEVLGDYR ACHGTLIKWI EETTAQQEMM KPGQAEDSRV
LSEQLSQQTD LFAEIERNQT KLDQCQKFSQ QYSTIVKDYE LQLMTYKAFV ESQQKSPGKR
RRMLSSSDAI TQEFMDLRTR YTALVTLTTQ HVKYISDALR RLEEEEKVVE EEKQEHVEKV
KELLVWVSTL ARNTQSKATS SQTKESTDIE KAILDQQVLA EELTTKKEQV SEAIKTSQIF
LAKHGHKLSE KEKEQISEQL DALNKAYHDL CDGSANQLQQ LQSQLAQQTE QKTLQKQQNT
CHQKLEDLCN WLGQAESTLA GHQGRTTQQD LSALQKNQSD LKDLQDDIQN RAASFASVVK
DIEGFLEENQ AKLSPHELTA LRKKLHQAKE QYEALQERTR VAQKELEEVV TSAVQQETEK
SKAAKELAEN RSKIDALLEW VTSVGLSGGQ MEQLSGASLG KEAFDTTDAH KGASQAPEEL
DKQCEKMKAR HQELLSQQQN FILATQSAQA FLDQHGHSLT PEERQVLQDK LGELKGQYAA
SLAQSEAELR QAQTLRDELQ KFLQDHQEFE NWLERSEREL EDMHKGGSCP EALPSLLKRQ
GSFSEDVISH KGDLRFVTIS GQKVLDTENS FEEGREPLGT GALVKGKLKD ATERYTALHS
KCTRLGSHLN MILGQYQQFQ SCTDCLQSWI QACEANVEKL LSDTIASDPG VLQQQLATTK
QLQEELAEHQ VAVEKLQKVA HDLMEIEGEP APDRKHVQET TDSILSRFQS LSSSLAERSA
LLQKAIARSQ SVQESLESLL QSIREVEQNL EEEQVASLSL GVIQEALATN MKLKQDIARQ
KSSLEATREM VTRFMETADS TTAAVLQGKL AEVSQRFKQL CLQQQEKESS LKKLLPQAEV
FEHLSDKLQQ FMENKSRMLA SGNQPDQDIA HFFQQIQELN SEMEDQQGNL ETLEHLVTGL
SSCGFALDLS QHQDRVQNLR KDFTELQKTV KEREEDASSC QEQLDEFRKL VRTFQKWLKE
TEGNIPPTET FLSAKELEKQ IEHLKGLLDD WASKGGLVEE INCKGTSLEN LIMEITAPDS
QAKTGSILPS VGSSVGSVNG YHTCKDLTEI QCDMSDVNLK YEKLGGVLRE RQESLQAVLS
RMQEVQKEAG SVLQWLESKE EVLKAMDASS SPTKPETVRA QADSNKAFLA ELEQNSPKIQ
KVKEALAGLL MTYPNSQEAE NWKEMQEELN SRWERATEAT MARQRQLEES ASHLASFQAA
ESQLRPWLME KELMMGVLGP LSIDPNMLTA QKQQVQFMLK EFEARRQQHK QLNEAAQGIL
TGPGNVSPST SQVQKELQSI NQKWIELTDK LNSRSSQIDQ AIVKSTQYQE LLQDLSEKVK
AVGQRLSGQS AISTQPEAVK QQLEETSEIR SDVEQLDQEI KEAQTLCDEL SVLIGEQYLK
DELKKRLETV ALPLQGLEDL AADRMNRLQA ALASTQQFQQ MFDELRTWLD DKQSQQAKNC
PISAKLERLQ SQLQENEEFQ KSLNQHSGSY EVIVAEGESL LLSVAPGEEK KTLQNQLVEL
KSHWEELSKK TADRQSRLKD CLQKAQKYQW HVQDLVPWIE DCKAKMSDLQ VTLDPVQLES
SLLRSKAMLS EVEKRRSLLE ILNSAADILI NSSETDEDDI RDEKAGINQN MDSITEELQA
KTGSLEEMTQ RLKEFQESFK NIEKRVEGAK HQLEIFDALG SQACSNKNLE KLRAQQEVLQ
TLEPQVDYLR NFTRGLVEDA PDGSDASQLL HQAEVAQQEF LEVKQRVNSG CMTMENKLEG
IGQFHCRVRE MFSQLADLDD ELDGMGAIGR DTDSLQSQIE DVRLFLHKIQ ALRLDIEASE
AECRQMLEEE GTLDLLGLKR ELEALNKQCG KLTERSRARQ EQLELTLGRV EDFYRKLKAL
NDMTTAAEEG EALQWVVGTE VDLINQQLAD FKMFQKEQVD PLQMKLQQVN GLGQGLIQSA
GKNCDVQGLE HDMEEINARW NTLNKKVAQR IAQLQEALLH CGKFQDALEP LLSWLADTEE
LIANQKPPSA EYKVVKAQIQ EQKLLQRLLD DRKATVDMLQ AEGGRIAQSA ELADREKITG
QLEGLESRWT GLLSKAAARQ KQLEDILVLA KQFHETAEPI SDFLSVTEKK LANSEPVGTQ
TAKIQQQIIR HKALEEEIES HATDVHQAVK IGQSLSSLTC SAEQGVLSEK LDSLQARYSE
IQDRGCRKAA LLEQALCNAR LFGEDEVEVL NWLAEVEDKL SSVFVKDYRQ DVLQKQHADH
LALNEEIINR KKNVDQAIKN GQALLKQTTG EEVLLIQEKL DGIKTRYADI TVTSSKALRT
LEQARQLATK FQSTYEELTG WLREVEEELA ASGGQSPTGE QIPQFQQRQK ELKKEVMEHR
LVLDTVNEVS RALLELVPWR AREGLDKLVS DANEQYKLVS DTVGQRVDEI DAAIQRSQQY
EQAADAELAW VAETKRKLMA LGPVRLEQDQ TTAQLQVQKA FSIDIIRHKD SMDELFSHRG
EIFGTCGDEQ KAVLQEKTES LIQQYEAISL LNSERYTRLE RAQVLVNQFW ETHEELSPWI
EETRALITQL PPPAIDHEQL RQQQEEMRQL RESIAEHKPH IDKLLKIGPQ LKELNPEEGE
MVEEKYQKAE NLYAQIKEEV RQRALALDEA VSQSAQIAEF HDKIEPMLET LENLSSRLRM
PPLIPAEVDK IRECISDNKS ATVELEKLQP SFEALKRCGE ELIGRSQGAD KDLAAKEIQD
KLDQMVFFWE DIKARAEERE IKFLDVLELA EKFWYDMAAL LTTIRDTQDI VHDLESPGID
PSIIKQQVEA AETIKEETDG LHEELEFIRI LGADLIFACG ETEKPEVKKS IDELNNAWES
LNKTWKERLE KLEDAMQAAV QYQDTLQAMF DWLDNAVIKL CTMPPVGTDL NTVKDQLNEM
KEFKVEVYQQ QIEMEKLNHQ GELMLKKATD ETDRDIIREP LMELKHLWEN LGEKIAHRQH
KLEGALLALG QFQHALEELM SWLTHTEELL DAQRPISGDP KVIEVELAKH HVLKNDVLAH
QATVETVNKA GNELLESSAG DDASSLRSRL ETMNQCWESV LQKTEEREQQ LQLTLQQAQG
FHSEIEDFLL ELTRMETQLS ASKPTGGLPE TAREQLDTHM ELYSQLKAKE ETYNQLLDKG
RLMLLSRDDS GSGSKTEQSV ALLEQKWHVV SSKMEERKSK LEEALNLATE FQNSLQEFIN
WLTLAEQNLN IASPPSLILN TVLSQIEEHK VFANEVNAHR DQIIELDQTG NQLKFLSQKQ
DVVLIKNLLV SVQSRWEKVV QRSIERGRSL DDARKRAKQF HEAWKKLIDW LEDAESHLDS
ELEISNDPDK IKLQLSKHKE FQKTLGGKQP VYDTTIRTGR ALKEKTLLPD DTQKLDNLLG
EVRDKWDTVC GKSVERQHKL EEALLFSGQF MDALQALVDW LYKVEPQLAE DQPVHGDLDL
VMNLMDAHKV FQKELGKRTG TVQVLKRSGR ELIENSRDDT TWVKGQLQEL STRWDTVCKL
SVSKQSRLEQ ALKQAEEFRD TVHMLLEWLS EAEQTLRFRG ALPDDTEALQ SLIDTHKEFM
KKVEEKRADV STAVAMGEAI LAACHPDCIT TIKHWITIIR ARFEEVLTWA KQHQQRLETA
LSELVANAEL LEELLAWIQW AETTLIQRDQ EPIPQNIDRV KALIAEHQTF MEEMTRKQPD
VDRVTKTYKR KNIEPAHTPF IEKPRGGSRK SLSQPTPPPM PILSQSEAKN PRINQLSARW
QQVWLLALER QRKLNDALDR LEELKEFANF DFDVWRKKYM RWMNHKKSRV MDFFRRIDKD
QDGKITRQEF IDGILASKFP TTKLEMTAVA DIFDRDGDGY IDYYEFVAAL HPNKDAYRPT
TDADKIEDEV TRQVAQCKCA KRFQVEQIGE NKYRFGDSQQ LRLVRILRST VMVRVGGGWM
ALDEFLVKND PCRARGRTNI ELREKFILPE GASQGMTPFR SRGRRSKPSS RAASPTRSSS
SASQSNHSCT SMPSSPATPA SGTKVIPSTG SKLKRPTPTF HSSRTSLAGD TSNSSSPVST
GAKTNRADPK KSASRPGSRA GSQAGSRASS RRGSDASDFD LLETQSACSD TSESSAAGGQ
GNSRRGLNKP SKIPTMSKKT TTASPRTLGP KR
//
