ID G1N2U8_MELGA Unreviewed; 652 AA.
AC G1N2U8;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 3.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase {ECO:0000256|ARBA:ARBA00012566};
DE EC=3.2.1.96 {ECO:0000256|ARBA:ARBA00012566};
OS Meleagris gallopavo (Wild turkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Meleagridinae; Meleagris.
OX NCBI_TaxID=9103 {ECO:0000313|Ensembl:ENSMGAP00000005990.3, ECO:0000313|Proteomes:UP000001645};
RN [1] {ECO:0000313|Ensembl:ENSMGAP00000005990.3, ECO:0000313|Proteomes:UP000001645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20838655; DOI=10.1371/journal.pbio.1000475;
RA Dalloul R.A., Long J.A., Zimin A.V., Aslam L., Beal K., Blomberg L.A.,
RA Bouffard P., Burt D.W., Crasta O., Crooijmans R.P., Cooper K.,
RA Coulombe R.A., De S., Delany M.E., Dodgson J.B., Dong J.J., Evans C.,
RA Frederickson K.M., Flicek P., Florea L., Folkerts O., Groenen M.A.,
RA Harkins T.T., Herrero J., Hoffmann S., Megens H.J., Jiang A., de Jong P.,
RA Kaiser P., Kim H., Kim K.W., Kim S., Langenberger D., Lee M.K., Lee T.,
RA Mane S., Marcais G., Marz M., McElroy A.P., Modise T., Nefedov M.,
RA Notredame C., Paton I.R., Payne W.S., Pertea G., Prickett D., Puiu D.,
RA Qioa D., Raineri E., Ruffier M., Salzberg S.L., Schatz M.C., Scheuring C.,
RA Schmidt C.J., Schroeder S., Searle S.M., Smith E.J., Smith J.,
RA Sonstegard T.S., Stadler P.F., Tafer H., Tu Z.J., Van Tassell C.P.,
RA Vilella A.J., Williams K.P., Yorke J.A., Zhang L., Zhang H.B., Zhang X.,
RA Zhang Y., Reed K.M.;
RT "Multi-platform next-generation sequencing of the domestic turkey
RT (Meleagris gallopavo): genome assembly and analysis.";
RL PLoS Biol. 8:E1000475-E1000475(2010).
RN [2] {ECO:0000313|Ensembl:ENSMGAP00000005990.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N(4)-(oligosaccharide-(1->3)-[oligosaccharide-(1->6)]-beta-
CC D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc)-L-asparaginyl-
CC [protein] + H2O = an oligosaccharide-(1->3)-[oligosaccharide-(1->6)]-
CC beta-D-Man-(1->4)-D-GlcNAc + N(4)-(N-acetyl-beta-D-glucosaminyl)-L-
CC asparaginyl-[protein]; Xref=Rhea:RHEA:73067, Rhea:RHEA-COMP:12603,
CC Rhea:RHEA-COMP:18176, ChEBI:CHEBI:15377, ChEBI:CHEBI:132248,
CC ChEBI:CHEBI:192714, ChEBI:CHEBI:192715; EC=3.2.1.96;
CC Evidence={ECO:0000256|ARBA:ARBA00034414};
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000256|ARBA:ARBA00004514}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 85 family.
CC {ECO:0000256|ARBA:ARBA00007849}.
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DR AlphaFoldDB; G1N2U8; -.
DR Ensembl; ENSMGAT00000006735.3; ENSMGAP00000005990.3; ENSMGAG00000006006.3.
DR GeneTree; ENSGT00390000018512; -.
DR HOGENOM; CLU_015297_0_0_1; -.
DR InParanoid; G1N2U8; -.
DR TreeFam; TF314391; -.
DR Proteomes; UP000001645; Chromosome 20.
DR Bgee; ENSMGAG00000006006; Expressed in jejunum and 13 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0033925; F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR CDD; cd06547; GH85_ENGase; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR032979; ENGase.
DR InterPro; IPR005201; Glyco_hydro_85.
DR PANTHER; PTHR13246:SF1; CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; 1.
DR PANTHER; PTHR13246; ENDO BETA N-ACETYLGLUCOSAMINIDASE; 1.
DR Pfam; PF03644; Glyco_hydro_85; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000001645}.
FT DOMAIN 125..400
FT /note="Glycoside hydrolase family 85"
FT /evidence="ECO:0000259|Pfam:PF03644"
SQ SEQUENCE 652 AA; 72871 MW; 2BB21D5F6532CEF2 CRC64;
MNQPWSLSLR AGSRRFHPQR LPWISCAEKE RKFIAAIILS HPPTHPDPID PSSILRLIAS
PARYFDTGTT EPISFFLSSL EELLAWQPNS DDDFNICAVP LAQRQPPLHS RRPRTLLCHD
MRGGYLEDRF IQGSATRNPY VFYHWRYIDI FVYFSHHTIT IPPVCWTNAA HRNGVPVLGT
FITEWMDGEK LCEAFLAGGE EAYRAVSHQL ARIAQHYRFD GWLINMENAL SVAAVGNLSP
FLRHLTAQVH GAVPGGLVIW YDSILESGTL RWQNELNEQN RVFFDACDGL FVNYNWKEEH
LERTRELAGQ RHADVYIGVD VFARGDVVGG GFDTNKSLSL IRKYDLSAAI FAPGWVYEHL
GEENFLQNEN KFWGLLEDYL PTHSICMLPL ATSFSVGMGT GMFLAGKEEE ARPWYNLSAQ
EIQPLYPERR GQLSTSCCLQ DAWCGGSSLR VQGTIPPGEE RVAIRLFSFQ MHAPPKLFLN
LLYKMEGPHG DDFTVALELT TWHSSRCHGS TVTVLPNEDE PHSRHHPRLL PAPPPALSRL
LAACSHGAQG WTSRCYEQEL RGCSLRDLSL LVSRQQASPQ ETSFSCLLGE LRVGAVHILT
AWWAANPATA MPGADGATFS STGAGCGQRG SLPTAGAERD SLPALVAGWP QR
//
