ID G1P5U5_MYOLU Unreviewed; 493 AA.
AC G1P5U5;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE SubName: Full=Dihydrouridine synthase 2 {ECO:0000313|Ensembl:ENSMLUP00000005402.2};
GN Name=DUS2 {ECO:0000313|Ensembl:ENSMLUP00000005402.2};
OS Myotis lucifugus (Little brown bat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Microchiroptera; Vespertilionidae;
OC Myotis.
OX NCBI_TaxID=59463 {ECO:0000313|Ensembl:ENSMLUP00000005402.2, ECO:0000313|Proteomes:UP000001074};
RN [1] {ECO:0000313|Ensembl:ENSMLUP00000005402.2, ECO:0000313|Proteomes:UP000001074}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSMLUP00000005402.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
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DR EMBL; AAPE02038704; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_014320453.1; XM_014464967.1.
DR AlphaFoldDB; G1P5U5; -.
DR STRING; 59463.ENSMLUP00000005402; -.
DR Ensembl; ENSMLUT00000005912.2; ENSMLUP00000005402.2; ENSMLUG00000005904.2.
DR GeneID; 102425792; -.
DR KEGG; mlf:102425792; -.
DR CTD; 54920; -.
DR eggNOG; KOG2334; Eukaryota.
DR GeneTree; ENSGT00550000075019; -.
DR HOGENOM; CLU_013299_3_0_1; -.
DR InParanoid; G1P5U5; -.
DR OMA; GPIRTNS; -.
DR OrthoDB; 276273at2759; -.
DR TreeFam; TF106151; -.
DR Proteomes; UP000001074; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:Ensembl.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:Ensembl.
DR GO; GO:0070402; F:NADPH binding; IEA:Ensembl.
DR GO; GO:0004860; F:protein kinase inhibitor activity; IEA:Ensembl.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:Ensembl.
DR GO; GO:0140374; P:antiviral innate immune response; IEA:Ensembl.
DR CDD; cd19871; DSRM_DUS2L; 1.
DR CDD; cd02801; DUS_like_FMN; 1.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR035587; DUS-like_FMN-bd.
DR InterPro; IPR044463; DUS2_DSRM.
DR InterPro; IPR018517; tRNA_hU_synthase_CS.
DR PANTHER; PTHR45936; TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE; 1.
DR PANTHER; PTHR45936:SF1; TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF01207; Dus; 1.
DR SMART; SM00358; DSRM; 1.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
DR PROSITE; PS01136; UPF0034; 1.
PE 4: Predicted;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000001074};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 370..435
FT /note="DRBM"
FT /evidence="ECO:0000259|SMART:SM00358"
FT REGION 442..493
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 443..460
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 493 AA; 55218 MW; 1F5532CCCCE7B731 CRC64;
MIGNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKML QCKRVVNEVL
STVDFVAPDD RVVFRTCQRE QSKVVFQMGT SDAERALAVA RLVENDVAGI DVNMGCPKEY
STKGGMGAAL LSDPDKIEKI LSTLVKGTSR PVTCKIRILP SLEDTLNLVK RIERTGIAAI
AVHGRKREER PQHPVSCETI KAIAETLSIP VIANGGSHDH IQDYLDIEDF RQATAASSVM
VARAAMWNPS IFLKEGLRPL EEVMQKYIRY AVQYDNHYTN TKHCLCQMLR EHLESPQGKL
LHAAQSSREI CEAFGLGAFY EETTRELDSR RAEFLARTPE EAEEPAEDTS GVIKMAVKFD
RRAYSPQITP KMCLLEWCRR EKLAQPVYET VQRPLDRLFC SVVTVAEQKY QSTLWDKSKK
LAEQAAAIVC LRSQGLPEGR LSEESLSLHK RKREASDQDP GGPRAQEPAM PGELCKKPLV
TLGSEKESPL EGW
//
