ID G1P820_MYOLU Unreviewed; 5602 AA.
AC G1P820;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN Name=MDN1 {ECO:0000313|Ensembl:ENSMLUP00000006294.2};
OS Myotis lucifugus (Little brown bat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Microchiroptera; Vespertilionidae;
OC Myotis.
OX NCBI_TaxID=59463 {ECO:0000313|Ensembl:ENSMLUP00000006294.2, ECO:0000313|Proteomes:UP000001074};
RN [1] {ECO:0000313|Ensembl:ENSMLUP00000006294.2, ECO:0000313|Proteomes:UP000001074}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSMLUP00000006294.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
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DR EMBL; AAPE02024031; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAPE02024032; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAPE02024033; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAPE02024034; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAPE02024035; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 59463.ENSMLUP00000006294; -.
DR Ensembl; ENSMLUT00000006889.2; ENSMLUP00000006294.2; ENSMLUG00000006776.2.
DR eggNOG; KOG1808; Eukaryota.
DR GeneTree; ENSGT00550000074802; -.
DR HOGENOM; CLU_000050_0_0_1; -.
DR InParanoid; G1P820; -.
DR OMA; ILEQWHR; -.
DR TreeFam; TF300488; -.
DR Proteomes; UP000001074; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0045111; C:intermediate filament cytoskeleton; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 3.
DR CDD; cd01460; vWA_midasin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 7.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR029317; KRBA1_rpt.
DR InterPro; IPR048617; MDN1_AAA_lid_4.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 7.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR Pfam; PF21108; MDN1_4th; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 6.
DR SMART; SM01258; KRBA1; 1.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340};
KW Reference proteome {ECO:0000313|Proteomes:UP000001074}.
FT DOMAIN 5390..5589
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 3989..4013
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4696..5269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4696..4739
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4754..4774
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4775..4802
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4805..4862
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4904..4929
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4943..4957
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4958..4974
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5089..5110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5112..5136
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5201..5218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 5235..5268
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5602 AA; 633309 MW; DF17F9012D541D7D CRC64;
MRHLLLVGAA APLSRCQERE SRSELGRFLA KQVWTPQDRQ CILNTLAQLL LDKDYTILIG
RQFRPILLDL LERNAEAIKA GGQVNHDLHE RLCVSMSKLI GSHPDVLPFA LRYFKDTSPV
FQRLFLESSD ANPVRYGRRR MKLRDLMEAA YKFLQQEQSV FRELWDWSVC VPLLRSHDTL
VRWYTAHCLA VVTCMNEEHK LSFLKKIFNS EELIHFRLRL LEEAQLQDLE KALVLANPEA
SLWRKEKELQ YLQGHLVSAD LSSRVTAVCG VVLPGQPPTP GEQASNRSSS HGQELAFRSY
VLVESVCKNL QTLAMAVASQ NAVLLEGPIG CGKTSLVEHL AAMTGRRKPP ELLKVQLGDQ
TDSKMLLGMY RCTDVPGEFV WQPGTLTQAA TKGHWILLED IDYAPLDVVS VLIPLLENGE
LLIPGRGDCL KVAPGFQFFA TRRLLSCGGN WYRPLSSHAT LLDKHWTKIH LDNMDKTELN
EVLQNRYPSL LAVTDHLLDI YTQLTGEKHS QSDSSVGCEQ VPKEVSEART ENKRLSLEGR
ELSLRDLLNW CNRIAHSFHS LSSSASLHIF HEALDCFTAM LSKHTSKLKM AEVIGSKLNI
SKKKVEFFCQ LYKPEIVINE LDVQVGRVRL LRKQSEAVHI QREKFTFAAT RPSSVLIEQL
AVCVSKGEPV LLVGETGTGK TSTVQYLAHI TGHRLRVVNM NQQSDTADLL GGYKPVDHKL
IWLPLREAFE ELFVQTFSKK QNFTFLGHIQ TCYRQKRWHD LLRLMQHVHK SAVNKDGKES
ETGLLLKEKW EAFGLRLDHA QQQMKMTENA LLFAFVEGTL AQAVKKGEWI LLDEINLAAP
ETLECLSGLL EGSSGSLVLL DRGDTEPLVR HPDFRLFACM NPATDVGKRN LPPGIRNRFT
ELYVEELESK EDLQILIVDY LKGLSVSKST VQGIINFYRD VRKESGRTLV DGTGHRPHYS
LRTLCRALRF AASNPCSNIQ RSLYEGFCLG FLTQLDRASH PIVQKLICQH IVSGSIKSLL
KQPIPEPKGG RLIQVEGYWI SMGDKEPTID ETYILTSSVK LNLRDIVRVV SAGTYPVLIQ
GETSVGKTSL IRWLAAATGN HCVRINNHEH TDIQEYIGCY TSDSSGKLVF KEGVLIDAMR
KGYWIILDEL NLAPTDVLEA LNRLLDDNRE LLITETQEVV KAHPRFMLFA TQNPPGLYGG
RKVLSRAFRN RFVELHFDEL PSSELETILH KRCSLPPSYC SKLVRVMLDL QHRRWNAIVY
LHSFTFMTLR DLFRWAERYR LAEQTKEEYD WLQHLANDGY MLLAGRVRKQ EEVDVIQEVL
EKHFKKKLCP RSLFSKENVL KLLSKLSTQK STLESKFSHI VWTESMRRLA VLVGRALKFS
EPVLLVGDTG CGKTSICQVF AALANQKLYS VNCHLHMETS DFLGGLRPVR QKPKDKEEID
TSRLFEWHDG PLVLAMKEGS FFLLDEISLA DDSVLERLNS VLEVEKSLVL AEKGSLEDKE
NEVELLTAGK KFRILATMNP GGDFGKKELS PALRNRFTEI WCPQSTNRED LKQIISSNLR
PGLSLGRIDH KGADIAEVML DFIDWLTHQE FGRRCVVSIR DILSWVNFMN TMGEEAALKR
PETISTVTTF VHAACLVYID GIGSGVTSSG FGTALLARKE CLKFLIKKLS KIVRLTESQK
NELKIYDRLK AKEFTGIDNR WGIHPFFIPR GPILHRNNIA DYALSAGTTA MNAQRLLRAT
KLNKPILLEG SPGVGKTSLV GALAKASGNT LVRINLSEQT DITDLFGADL PVEGGKGGEF
AWRDGPLLAA LKAGHWVVLD ELNLASQSVL EGLNACFDHR GEIYIPELGM SFQVQHEKTK
IFGCQNPFRQ GGGRKGLPRS FLNRFTQVFV DPLTVIDMEF IASTLFPAID KNIVKKMVAF
NNQIDHEVTV EKKWGQKGGP WEFNLRDLFR WCQLMLVDQS PGCYDPGQHV FLVYGERMRT
REDKEKVIAI FKDVFSSDSN PYMGTRLFHI TPYDVQLGYS VLCRGGYVTP PSRHPLLLLH
QSLQSLESIM KCVQMSWMVI LVGPASVGKT SLVQLLAQLT GHTLKIMAMN SAMDTTELLG
GFEQVDLIRP WRQLLEKVES TVRALLRDSL LVSADDAEVV LRAWSHFLLT YKPKCLGEDG
KGVTMEIVNK LEAVLLLMQR LNNKINSYSK AEFAKLVEEF RSFGAKLVQS SSGGSHGTFE
WVDSMLVRAL KSGDWLLMDN VNFCNPSVLD RLNALLEPGG VLTVSERGMI DGSTPTITPH
PNFRLFLSMD PVHGEISRAM RNRGLEIYIS GEGDGSIPDD LDLKVLLHSL GLVGDSVCDT
LLALHRETQS AFVGSPTSSI STLIQTALLI VQYLQRGLSL DRAFSEACWK AYVRSQHSPA
NQKLGQALLE KHISSLRAHE TWGNSILAVG LWPDSLPSAL FAMEDSHLSI VRSDGQILAY
CLNRMSMKTS SWARIQPLTL PDLEKIMQSP NPESLKFSSV EVDTLWIDEP EVLAMAVKLL
IERATNQDWM LRVKWLYHLA KNIPQGLESI QIHLEASAAS LRKFYSNSLS AGISNVIKIL
QPNITEECVI PLDPRWNMQA LDIIRSSMDF DPQMDQSEQL FALLESIANK TIIYLDREKR
IFTEANLASV GGKKLRNSVL RMSFEFHKDP ESYQSLPHEI VVNLAAFFEL CDALILLWVQ
SSQGIVSDAN VNEILASLRW RDRFWTVADT VKVDAPGLAL LALHWHWVLK HLVRQIPQLL
MNHEDKYYKE VQTVSEHIQN CLGSPTGGFT GIKKLQKFLG RPFPFKDKLV VECFSQLKTL
NKALAIRERM AVMGESVWLE DISRLQVVAS EWTLKKSLLQ AWGLVLRANI LQDTNPDELK
NLVNAQCLEV KAKGISLGFL ERKPSDASTP SQPDFTSLIQ LTRSVQLWPA MEYLAVLWQY
KVTADFMTQA CLRRNRSSKN QQPQIDEEIS NHITFCLKHT PVAPQELRGL WSLLHHQKVS
TEEITSLWSD LFNSTFMSFW SSTVTTNPEY WLTWSPLTNA QQRERPKSLL DSTLKGPGSL
SRAMFSKCCF EVLTSSCRAS PWDVSGLPIL SSSHVTLGEW VERAQQLRDI SSVLWTNMAV
PSVAEFRRTD SRLQGLVLCR HLAGLAELLP ETLRQEYMHN CEQLLRGDSQ AFQHVGQTLG
DMAGQELLPK DLLCLLLTSL RLLFGEGEGK RDLPEPARRG SLWVSLGLLQ IQTWLPQARF
DPAVKREYKL RYAKEELYQL QCEWKTRNLS SELQTGRDLE DEVIINYSHP HIRLLYQRID
QLENLICSLS KKQAFRPQLP AYESLVQEIH HYISSIAKAP AVQDLLTRLL QALQMDGLRS
AQVAQNLLKE EASWQQSHHQ FRRRLAEEYA LYPDSVTPLQ ASILQMQHGM RLVASEVHSS
LHSSVVCADR LDTLVASLLA FPSVGPTFPT YYAHADALCS VKSEEVLRGL GKLTLKRSGG
KELESKSQRP CPTREQLLLN ALLYLRSHVL CKGELDQRAL QLFRHVCQEI INEWDEQERL
AQEKAEQENS LYRYRSRSSR TALSEEEEEE REFRRQFPLH QQDFADILVE PTLEEKGTSD
GQEAAAATDP TLLSPSSMQA VMLIHQQLCL SFARSLWYQQ TLPPHEAKHY LSLFLSCYQT
GASLVTHFYP LMGVDLNAQL LGSQLLACTL SHNTLFGEAT SDLIVKPDGP YDFYQHPNVP
EARLCQPVLQ GFSEAVIRLL QDWPEHPALV QLMVVMDRIR SFPLSSPISK FLNGLEILLA
KAQDWEENAS RALSLRKHLD LVSQMIIRWR KLELNCWSMS LDNTMKRHTE KSTKHWFSIY
QMLEKHMQEQ TEEQEDDKQM TLMLLVSTLQ AFIEGSSLGE FHVRLQMLLV FHCHVLLMPQ
VEGKDSLCSV LWNLYHYYKQ FFDRVQAKIV ELRSPLEKEL KEFVKISKWN DVSFWSIKQS
VEKTHRARTL FKFMKKFEAV LSEPCQSSLV ESDKEEQPDF LPQPTEAATS EASPIQNLNK
ALRETLLARP AAAQATVPEQ CLGAPFPSEG ELLYRLPKLT KRMRKMCLTL MKESRLPHLV
DGLDQFTGEV ISSVRELQSL QVEPSAEKEK QRSEAKHILM QKQRALADLF KHLAKTGLSY
RKGLAWARSK NPQEMLHLRP LDLRSALSIV SSTQEADSRL LTEISSSWDG CQKYFYRSLA
RHARLSAALA APVKEMGVGS IERCKGFSAH LMKMLIRQRR SLTTLTEQWI LLRNLLSCVQ
EIKGRLTGPL LYPVAFPPQD GVQQWTERLQ HLAMQSQILL EQLSWLLQCC PDTASAPSQG
DARAQGQSST LCPEGPELTT GQLSRAVLDF SPSDLSYPSP VPGNQLPSGC RMRKQDQLWQ
QSTSRLTEML KTIQTVKADV DRIRQQSCET LFHSWKDFEV CSSGLSCLSQ VSAHLQGLES
LFILPGMEVE QTDQQMALVE SLEYVRGEIG KATDDFTTWK THLLTSGSQG GNQLLDEGFV
EDFAEKMETA IRSILYAIQN LAERNSKKTD ENTDQIRPQE EDAGFERLQS GHLTKLLEED
LWADVSTLHV QKIISAISEL LERLKSCGED GTAAKHMFFS QPCCWLVRLL PMLSRYSDLV
LFFLTMSLAT HRSTAKLLSV LTQVFTELAQ KGFCLPKEFM EDSAGEGATE FHDYEGGGIG
EGEGMKDVSD KIENEEQAED TFQKGQEKDK EDHDSKSDIK GEDNAIEMSE DFDGKMHDGE
LEEQEEEDEK SDSEGGDLDK QMGDLNGEEA DKLDERLWGD DEEEEDEEEE DSKTEETGPG
MDEEDCGLVA KDDNLDSGKS NRDKNQHDKK EEAEADDDGQ GQDKINEQID EREYDESEVD
PYHGNQETLP EPEALDLPED LNLDSEDKNS GEDTDQEEGE VYPEENPLEI KEKAMDTEEA
GHEAEEINEE TGANQNEDQG QQEPEEGSSE DDKGEGVEEM DTGASDQNKD MDQPSEESSE
EEEPSPEDKD NNAEASEDSA ENGVSVDQGL QPQEEKEEGE DSDTEEQVPE AMERKEHSSC
GQTGVENMQS EQAVELAGAA PEKELGKEEH GSGAADANQA EGHESNVIAR LASQKQTRKN
TQSFKRKPGQ ANNERSLGDH NEHVHKRLRT VDMDSHTEQE PTPAQPQAQA EDAAAYEHIK
KGSDPYDAQT YDVASMEQQQ SAKDSSKEQE EEEREDIFMD VEEQEELKAV DTEQLKPEEV
KSGTTAGSGV DEMETTTVKT EEDQDPRTDR SHKETENEKP ERSRDSTIHT AHQFLVDTIF
QPFLKDVDEL RQELERQLER WQPHEPGNPE EEKAAAEMWQ SYLILTAPLS QQLCEQLRLI
LEPTQAAKLK GDYRTGKRLN MRKVIPYIAS QFRKDKIWLR RTKPSKRQYQ ICLAIDDSSS
MVDNHTKQLA FESLAVIGNA LTLLEVGQIA VCSFGESVKL LHPFHEQFSD YSGSQILRLC
KFQQKKTKIA QFLESVASMF AAAQQLSQNV SPDAAQLLLV VSDGRGLFLE GKDRVLAAVQ
AARNANIFVI FVVLDNPSSR DSILDIKVPI FKGPGELPEI RSYMEEFPFP FYIILRDVNA
LPETLSDALR QWFELVTASD HL
//
