ID G1PA61_MYOLU Unreviewed; 2536 AA.
AC G1PA61;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HERC2 {ECO:0000313|Ensembl:ENSMLUP00000007158.2};
OS Myotis lucifugus (Little brown bat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Chiroptera; Microchiroptera; Vespertilionidae;
OC Myotis.
OX NCBI_TaxID=59463 {ECO:0000313|Ensembl:ENSMLUP00000007158.2, ECO:0000313|Proteomes:UP000001074};
RN [1] {ECO:0000313|Ensembl:ENSMLUP00000007158.2, ECO:0000313|Proteomes:UP000001074}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSMLUP00000007158.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; AAPE02060074; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 59463.ENSMLUP00000007158; -.
DR Ensembl; ENSMLUT00000007837.2; ENSMLUP00000007158.2; ENSMLUG00000007711.2.
DR eggNOG; KOG1426; Eukaryota.
DR GeneTree; ENSGT00940000154975; -.
DR HOGENOM; CLU_228287_0_0_1; -.
DR InParanoid; G1PA61; -.
DR OMA; QXDIEAK; -.
DR TreeFam; TF320636; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000001074; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd08664; APC10-HERC2; 1.
DR CDD; cd14402; UBA_HERC2; 1.
DR CDD; cd02344; ZZ_HERC2; 1.
DR Gene3D; 2.30.30.30; -; 1.
DR Gene3D; 3.30.60.90; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.130.10.30; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; 2.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR021097; CPH_domain.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR037976; HERC2_APC10.
DR InterPro; IPR010606; Mib_Herc2.
DR InterPro; IPR037252; Mib_Herc2_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR InterPro; IPR014722; Rib_uL2_dom2.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR000433; Znf_ZZ.
DR InterPro; IPR043145; Znf_ZZ_sf.
DR InterPro; IPR041987; ZZ_HERC2.
DR PANTHER; PTHR22872:SF2; BTB_POZ DOMAIN-CONTAINING PROTEIN 1; 1.
DR PANTHER; PTHR22872; BTK-BINDING PROTEIN-RELATED; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF11515; Cul7; 1.
DR Pfam; PF06701; MIB_HERC2; 1.
DR Pfam; PF00415; RCC1; 10.
DR Pfam; PF00569; ZZ; 1.
DR PRINTS; PR00633; RCCNDNSATION.
DR SMART; SM01337; APC10; 1.
DR SMART; SM00291; ZnF_ZZ; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF159034; Mib/herc2 domain-like; 1.
DR SUPFAM; SSF50985; RCC1/BLIP-II; 2.
DR SUPFAM; SSF63748; Tudor/PWWP/MBT; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS51416; MIB_HERC2; 1.
DR PROSITE; PS00626; RCC1_2; 1.
DR PROSITE; PS50012; RCC1_3; 11.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS01357; ZF_ZZ_1; 1.
DR PROSITE; PS50135; ZF_ZZ_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001074};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00228}.
FT DOMAIN 257..330
FT /note="MIB/HERC2"
FT /evidence="ECO:0000259|PROSITE:PS51416"
FT DOMAIN 847..902
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 1100..1152
FT /note="ZZ-type"
FT /evidence="ECO:0000259|PROSITE:PS50135"
FT DOMAIN 1156..1333
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT REPEAT 1356..1407
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1408..1462
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1463..1514
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1515..1566
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1569..1620
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1621..1672
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1673..1724
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 2351..2402
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 2403..2456
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 2457..2508
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 2509..2536
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REGION 331..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 743..771
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2001..2028
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2536 AA; 272987 MW; FDD71841144972E3 CRC64;
QDKWQPLLST VAGVHRYKWL KQSAQGLPPQ SALLGTIAEF ALREEPVDVE KMRKCLLKQL
ERAEVRLEGI DTILKLAAKS FLLPSVQYAV FCGWQRLIPE GVDLGEPLTD CLKDVDLIPP
FNRMLLEVTF GKLYAWAVQN IRNILMDANA KFKELGIQPV PLQTVTNENP AGPSLGTTPH
ARFLLAMLSL LTLQHGAHNL TLLLNSGTLA LAQTALRLIG PSGDHVEEDG NVSARGASAT
VLEETRKDTA PAQLPVSGPE LAAMMKIGTR VMRGVDWKWG DQDGPPPGLG RVIGELGEDG
WIRVQWDTGS TNSYRMGKEG KYDLKLAELP ASAQPSAEDS DTEDDSEAEP ADRNMHPTAM
MLTSTVRLLQ TLCLSAGVHA EVMQGEATRT LCGLLRVVVE SGTTDTTPAP NSAVCREQHR
GWCSLGFVRS IALTPHMCAA LSSPPWIALL MRIVEGHAPF TAASLQRQIL ALHLLQAVLP
SWDKAERAED MEGLVEKLFG FLGSLLTTCS SDVPFLREST LRRRRARPQA SLTATHSSTL
AEEAVALLRT LHSLGQWNGL INKHINSQLQ AVSRSCGPTP AGGALLEDCL PDADSPEVGG
LMAVLAVIGG IDGRLRLGGQ VAHEEFGEGT VTRITPKGRI TVQFSDMRTC RVCPLSQLKP
LPAVPFSITS LPFTDPMLAV WAQLVNLAGS RLEKHKIKKS PKQGFAGPVD LGLLRRQQLK
LYVLKAGRAL LPHQDQLRQT LAQPAVQEAA AQADDGAAAS PDLGDMSPEG PQPPMILLQQ
LLAAATQPSP VKAVFARQEL EAAALAVCQY LAVECAHPSS PAFEDGSSSE ASTPVPAQHL
RPIRARKQRQ SPVPTLPIVV QLMEMGFPRR NIELALKSLT GASGNAAGLP GVEALVGWLL
DHADAPGTDL SDPDTGSEDG SDEELLEDLD DTAYALSSGA VVTESQTYKK RADFLSNDDY
AVYVRENIQV GMMVRCCRTY EEVCEGDVGK VIKLDRDGLH DLNVQSDWQH KGGTYWVRYI
HVELLGYPPP SFPAHIKIGD KVRVKAAVTT PKYKWGSVTH QSVGVVKAFS ANGKDVIVDF
PQQSHWTGLL SEMELVPSVH PGVTCDGCQV FPINGSRFKC RNCDDFDFCE PCFKTRRHSP
RHTFGRINEP GQSAVFCGRS GKQLKRGPSS QPGALLDSWS RMVKSLHVSS SVNQASRLID
GSEPCWQSSG SQGKHWIRLE IFPDVLVHRL KMTVDPADSS YMPSLVVVSG GNSLNNLIEL
KTINISPTDT TVSLLSDCTE YHRYIEIAIK QCRSSGIDCK IHGLVLLGRV RAEEEDWAAV
PFLASDNEEE EDEKGGAGSL IRKKAAGLES AATIRTKVFV WGLNDKDQLG GLKGSKIKVP
SFSETLSALN VVQVAGGSKS LFAVTVEGKV YACGEATNGR LGLGMSSGTV PIPRQVTALS
SYVVKKVAVH SGGRHATALT VDGKVFSWGE GDDGKLGHFS RMNCDKPRLI EALKTKRIRD
IACGSSHSAA LTSSGELYTW GLGEYGRLGH GDNTTQLKPK MVKVLLGHRV IQVACGSRDA
QTLALTDEGL VFSWGDGDFG KLGRGGSEGC NVPQNIERLN GQGVCQIECG AQFSLALTKS
GVVWTWGKGD YFRLGHGSDV HVRKPQVVEG LRGKKIVHVA VGALHCLAVT DAGQVYAWGD
NDHGQQGNGT TTVNRKPTLV QGLEGQKITR VACGSSHSVA WTTVDMATPS VHEPVLFQTA
RDPLGASYLG VPSDAESSAA NNKLSGAGSS KPGRPSLAKT LLSLDGNLAK QQALSHVLNA
LQITYARDAV VGALMPASMI APECPSFSSS APPSEASATA SPVSGEACLL AADPEDRPSP
SPWQEKRGEV IISSEDAVTP AAVTPSASSA SSRPFIPVTD DPGAASIIAE TMTKAKEDVE
SQNKASGPEP QALDEFTSLL VADDTRVLVD LLKLSVCGRA GDRGREVLSA VLSGMGTAYP
QVADMLLELC VTELEDVASD SQSGRLSSQP VVVESSHPYT DDTSSSGTVK IPGAEGLRVE
FDRQCSTERR HDPLTVMDGV NRIVSVRSGR EWSDWSSELR IPGDELKWKF ISDGSVNGWG
WRFTVYPIMP AAGPKDLLSD RCVLSCPSMD LVTCLLDFRL NLASNRSIVP RLAASLAACA
QLSALAASHR MWALQRLRKL LTTEFGQSVN IRRLLGDSDG EARALSFTGS ALAALVKGLP
ETLERQFEYE DPVVRGGKQL LHSPFFKVLV ALACDLELDT LPCCAETHKW AWFRRYCMAS
RVAVALDRRT PLPRLFLDEV AKKIRELMAD GEAMDVLHES HDVFRREHDE QLVQWMNRRP
DDWTLSAGGS GTIYGWGHNH RGQLGGIEGA KVKVPTPCEA LATLRPVQLI GGEQTLFAVT
ADGKLYATGY GAGGRLGVGG TESVSTPTLL ESIQHVFIRK VAVNSGGKHC LALSSEGEVY
SWGEAEDGKL GHGNRSPCDR PRVVESLRGT EVVDIAAGGA HSACVTSAGD LYTWGKGRYG
RLGHSDSEDQ LKPKLV
//
