ID G1SD00_RABIT Unreviewed; 1925 AA.
AC G1SD00;
DT 19-OCT-2011, integrated into UniProtKB/TrEMBL.
DT 19-OCT-2011, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=Lactase-phlorizin hydrolase {ECO:0008006|Google:ProtNLM};
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986 {ECO:0000313|Ensembl:ENSOCUP00000000258.2, ECO:0000313|Proteomes:UP000001811};
RN [1] {ECO:0000313|Ensembl:ENSOCUP00000000258.2, ECO:0000313|Proteomes:UP000001811}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thorbecke inbred {ECO:0000313|Ensembl:ENSOCUP00000000258.2,
RC ECO:0000313|Proteomes:UP000001811};
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSOCUP00000000258.2}
RP IDENTIFICATION.
RC STRAIN=Thorbecke {ECO:0000313|Ensembl:ENSOCUP00000000258.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR EMBL; AAGW02005092; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAGW02005093; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAGW02005094; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_002712474.1; XM_002712428.3.
DR SMR; G1SD00; -.
DR STRING; 9986.ENSOCUP00000000258; -.
DR PaxDb; 9986-ENSOCUP00000000258; -.
DR Ensembl; ENSOCUT00000000293.2; ENSOCUP00000000258.2; ENSOCUG00000000293.2.
DR GeneID; 100352497; -.
DR KEGG; ocu:100352497; -.
DR CTD; 3938; -.
DR eggNOG; KOG0626; Eukaryota.
DR GeneTree; ENSGT00940000155324; -.
DR HOGENOM; CLU_001859_5_3_1; -.
DR InParanoid; G1SD00; -.
DR OMA; QITHYKV; -.
DR OrthoDB; 3373839at2759; -.
DR TreeFam; TF314803; -.
DR Proteomes; UP000001811; Chromosome 7.
DR Bgee; ENSOCUG00000000293; Expressed in testis and 2 other cell types or tissues.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 4.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR10353:SF36; KLOTHO (MAMMALIAN AGING-ASSOCIATED PROTEIN) HOMOLOG; 1.
DR Pfam; PF00232; Glyco_hydro_1; 4.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 4.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 2.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 3.
PE 4: Predicted;
KW Glycosidase {ECO:0000256|RuleBase:RU004468};
KW Hydrolase {ECO:0000256|RuleBase:RU004468};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000001811};
KW Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..1925
FT /note="Lactase-phlorizin hydrolase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003421697"
FT TRANSMEM 1882..1905
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 1218..1237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1271
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
FT ACT_SITE 1746
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ SEQUENCE 1925 AA; 217896 MW; 704E83204B01841E CRC64;
MELFWSIVFT VLLSFSCRGS DWESDSNFIS AAGPLTTDLL LSLQYPQGNQ TSDFAAGGKD
LYVCSQPLPA FLPEYFSSLH ASEITHYKVF LSWAQLLPAG RSGDPDENAV RCYRQLLEAL
RAAQLQPMVV LHHQPLPASS ALRSDVFANL FAEYATFAFH AFGDLVGVWL TFSDLEAAIR
ELPQQESRAW RLQLLTEAHR KAYEIYHQKY AAQGGKVSVV LQAEEISELL LESSTSALAK
DSIDFLSLDL SYECQSETSL PEKLSKLQTI EPKVKVFIFT LRLQDCPSSR KSPASLLFSF
IEAINKDQML TLGFDVNVFL RCSSTSKKSI SCFLTDSLAL QTDHERAARN SAPASAYQRV
WEMFAHQPRA ERDAFLQDAF PQGFLWGVST GAFNVEGGWA EGGRGPSVWD QFGHLKAAQG
QATPEVASDS YYKWASDVAL LRGLRAQVYK FSISWSRIFP MGRGSSPSPQ GVAYYNKLID
SLLDSHIEPM ATLFHWDLPQ ALQDEGGWQN ESVVDAFVDY AAFCFSAFGN RVKLWVTFHE
PWVMSYAGYG TGQHAPGISD PGIASFQVAH LVLKAHARTW HHYNSHHRPQ QQGRVGIVLN
SDWAEPLSPE RPEDLAASER FLHFMLGWFA HPIFVDGDYP ATMKAQIQQR NEQCPSPVAQ
LPEFTDTEKQ LLKGSADFLG LSHYTSRLIS KAPEDSCIPS YDTIGGFSQH TDPAWPQTSS
PWIRVVPWGI RRLLQFVSLE YTKGKVPIYL AGNGMPIGES ENLLNDSLRV DYFNQYINEV
LKAIREDSVD VRSYIARSLM DGFEGPAGYS QRFGLYHVNF NESSKPRTPR KSAFLLTSII
EKNGFLTKAV KQPLPPNSAH LPSKTRASAL PSEVPSKAKV VWEKFSNQTK FERDLFYHGT
FRDDFLWGVS SSAYQIEGAW DADGKGPSIW DNFTHTPGNG VTDSSTGDIA CDSYNQLDAD
LNVLRALKVK AYRFSLSWSR IFPTGTNSSI NSHGVDYYNR LIDGLLASDI FPMVTLFHWD
LPQALQDIGG WENPSLIDLF DSYADYCFQT FGDRVKFWIT LNEPTYYSWW SYGSGTFPPN
VNDPGWAPYR ISHALIKAHA RVYHTYDEKY RQSQNGVISL SLVAQWAEPK SPDVLRDVEA
ADRKMQFTLG WYAHPIFKTG DYPDAMKWKV GNRSELQHLA TSRLPSFTEE EKSYIRGTAD
VFCLNTYSSK IVQHKTPALN PPSYEDDQEL AEEEDTSWPT TAMNRAASFG MRRLLNWIKE
EYGDIPIYIT ENGVGLTNPR LEDIDRIFYY KTYINEALKA YRLDGVNLRG YFAWSLMDNF
EWLRGYTVKF GLYHVDFEDV NRPRTARISA GYYTELITNN GMPLPSEDEF MYGQFPEGFI
WSAATSAYQI EGAWRADGKS LSIWDTFTHT RLRIENDDIA DVACDSYHKI SEDVVALQNL
AVTHYRFSIS WSRILPDGTT NYINEAGLNY YVRLIDALLA ANIKPQVTLY HWDLPQALQD
VGGWENETIV QRFKEYADVL FQRLGDKVKF WITLNEPYVI ANQGYGYGVS APGISFRPGT
APYIVGHNLI KAHAEAWHLY NDVYRASQGG VISITISSDW AEPRDPSNQE DVEAANRYVQ
FLGGWFAHPI FKNGDYNEVM KTRIRERSLA AGRNVSRLPE FTESEKRRIN GTYDFFGFNH
YTTVLAYNLN YPIISSYDAD RGVASIVDRS WPDSGSFWLK MTPFGFRRIL NWIKEEYNNP
PIYVTENGVS HRGDSYLNDT TRIYYLRSYI NEALKAVQQD KVDLRGYTVW TLMDNFEWAT
GFSDKFGLHF VNYSDPSLPR IPRESAKFYA SIVRCNGFPD PAEGPHPCLL QPEDTDPTMS
PVSQEEVQFL GLSLGSTEAE TALYVLFSLM LLGVCGLAFL SYALCKSSKQ RKKLSQQELS
PVSSF
//
