ID G1XWR5_9PROT Unreviewed; 783 AA.
AC G1XWR5;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=Nitrogen regulation protein {ECO:0000256|PIRNR:PIRNR037532};
DE EC=2.7.13.3 {ECO:0000256|PIRNR:PIRNR037532};
GN Name=ntrY {ECO:0000313|EMBL:EGY02290.1};
GN ORFNames=AZA_87994 {ECO:0000313|EMBL:EGY02290.1};
OS Nitrospirillum amazonense Y2.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Nitrospirillum.
OX NCBI_TaxID=1003237 {ECO:0000313|EMBL:EGY02290.1, ECO:0000313|Proteomes:UP000018435};
RN [1] {ECO:0000313|EMBL:EGY02290.1, ECO:0000313|Proteomes:UP000018435}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Y2 (ATCC 35120) {ECO:0000313|Proteomes:UP000018435};
RX PubMed=21838888; DOI=10.1186/1471-2164-12-409;
RA Sant'anna F.H., Almeida L.G., Cecagno R., Reolon L.A., Siqueira F.M.,
RA Machado M.R., Vasconcelos A.T., Schrank I.S.;
RT "Genomic insights into the versatility of the plant growth-promoting
RT bacterium Azospirillum amazonense.";
RL BMC Genomics 12:409-409(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000256|ARBA:ARBA00000085,
CC ECO:0000256|PIRNR:PIRNR037532};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|PIRNR:PIRNR037532};
CC Multi-pass membrane protein {ECO:0000256|PIRNR:PIRNR037532}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGY02290.1}.
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DR EMBL; AFBX01000105; EGY02290.1; -; Genomic_DNA.
DR RefSeq; WP_004271809.1; NZ_AFBX01000105.1.
DR AlphaFoldDB; G1XWR5; -.
DR PATRIC; fig|1003237.3.peg.734; -.
DR Proteomes; UP000018435; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR CDD; cd06225; HAMP; 1.
DR CDD; cd00082; HisKA; 1.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 1.10.287.130; -; 1.
DR Gene3D; 6.10.340.10; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR017232; NtrY.
DR InterPro; IPR045671; NtrY-like_N.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR013767; PAS_fold.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR PANTHER; PTHR43065:SF10; PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3; 1.
DR PANTHER; PTHR43065; SENSOR HISTIDINE KINASE; 1.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF19312; NtrY_N; 1.
DR Pfam; PF00989; PAS; 1.
DR PIRSF; PIRSF037532; STHK_NtrY; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00304; HAMP; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF158472; HAMP domain-like; 1.
DR SUPFAM; SSF47384; Homodimeric domain of signal transducing histidine kinase; 1.
DR SUPFAM; SSF55785; PYP-like sensor domain (PAS domain); 1.
DR PROSITE; PS50885; HAMP; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|PIRNR:PIRNR037532};
KW Cell membrane {ECO:0000256|PIRNR:PIRNR037532};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|PIRNR:PIRNR037532};
KW Membrane {ECO:0000256|PIRNR:PIRNR037532, ECO:0000256|SAM:Phobius};
KW Nitrogen fixation {ECO:0000256|PIRNR:PIRNR037532};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR037532};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000018435};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR037532};
KW Transmembrane {ECO:0000256|PIRNR:PIRNR037532, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Two-component regulatory system {ECO:0000256|PIRNR:PIRNR037532}.
FT TRANSMEM 44..63
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 116..143
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 187..206
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 320..338
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 342..397
FT /note="HAMP"
FT /evidence="ECO:0000259|PROSITE:PS50885"
FT DOMAIN 535..758
FT /note="Histidine kinase"
FT /evidence="ECO:0000259|PROSITE:PS50109"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..22
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 783 AA; 85896 MW; 7824927DDD2AE79F CRC64;
MTEVPPALIT PPSPPVPPQV PPKSDSWWRR LSVWSARLGL GNKLAVALAF AAVLSGFATY
AALTQQPPFG NSPNTVSLLL TLDGALLLLL LALMARRIVG LWIERRRGLA GSRLHVRLVA
VFSLLAVLPA IVMAAFSAFF FYLGLQSWFS DRVRTAVNES LVVAQAYLDE HQIAIRNDAS
WVANDLLAAR QGPLVTLDPL FIYAFLRNES FQRSFTEAVI FDGQGRQYGS YTLGSVFQPD
VMPKERLERA RKGEVDFEFN EKEKRVTALI RLDPDFDIFL LLGRPVEAKV MEHLNAAQTA
VREYTELEGQ RFGLQTKMTL IFIIVSLLLL LVAIWAGLNF ANTLITPISA LIIAAERIRT
GDLTARVPET LPGPEDELTS LSRAFNRMTS QLSSQRSDLV EANRQLDLRR RFTEAVLAGV
SAGVIGLDEQ GFINLPNQSA AQLLDIPDVH EMMGRDILAV VPELEDLMSA IRRRPSRLVE
GQVQIRRGNT QRTLLVRVAA DTSGNGIRGF VITFDDVSEL LSAQRKAAWA DVARRIAHEI
KNPLTPIQLS AERLRRKYLK EIQSDPETFK AMTDTIVRHV DDIGRMVDEF SAFARMPTPV
MKPQNIQELC RQAVFLQATA QSGIKFDSIL PPEKVEVECD GRQISQALTN LLKNAVEAIE
GRMEEDAADG HDSPPGHISL KVEALDDHIV LTIADNGRGL PSEGRDRLTE PYVTTRAKGT
GLGLAIVKKI LEDHGGTLGL DDNPGGGARV TLILPLVQAA APQDAETAAS PVLVAGTSAS
KGV
//
