ID G3HCB2_CRIGR Unreviewed; 1372 AA.
AC G3HCB2;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 67.
DE RecName: Full=ribonuclease III {ECO:0000256|ARBA:ARBA00012177};
DE EC=3.1.26.3 {ECO:0000256|ARBA:ARBA00012177};
GN Name=Drosha {ECO:0000313|Ensembl:ENSCGRP00001006583.1};
GN ORFNames=I79_008119 {ECO:0000313|EMBL:EGW01612.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:EGW01612.1, ECO:0000313|Proteomes:UP000001075};
RN [1] {ECO:0000313|Proteomes:UP000001075}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHO K1 cell line {ECO:0000313|Proteomes:UP000001075};
RX PubMed=21804562; DOI=10.1038/nbt.1932;
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan H.C., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.";
RL Nat. Biotechnol. 29:735-741(2011).
RN [2] {ECO:0000313|EMBL:EGW01612.1}
RP NUCLEOTIDE SEQUENCE.
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan C.H., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary CHO-K1 cell line.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Ensembl:ENSCGRP00001006583.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.3;
CC Evidence={ECO:0000256|ARBA:ARBA00000109};
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DR EMBL; JH000279; EGW01612.1; -; Genomic_DNA.
DR RefSeq; XP_003501752.1; XM_003501704.2.
DR RefSeq; XP_007640553.1; XM_007642363.1.
DR RefSeq; XP_016830723.1; XM_016975234.1.
DR STRING; 10029.G3HCB2; -.
DR PaxDb; 10029-XP_007631627-1; -.
DR Ensembl; ENSCGRT00001010369.1; ENSCGRP00001006583.1; ENSCGRG00001008885.1.
DR GeneID; 100766983; -.
DR CTD; 29102; -.
DR eggNOG; KOG1817; Eukaryota.
DR GeneTree; ENSGT00730000111052; -.
DR OMA; ENFTIHE; -.
DR OrthoDB; 5486676at2759; -.
DR Proteomes; UP000001075; Unassembled WGS sequence.
DR Proteomes; UP000694386; Unplaced.
DR GO; GO:0004525; F:ribonuclease III activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0031054; P:pre-miRNA processing; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:InterPro.
DR CDD; cd19877; DSRM_RNAse_III_meta_like; 1.
DR CDD; cd00593; RIBOc; 2.
DR Gene3D; 3.30.160.20; -; 1.
DR Gene3D; 1.10.1520.10; Ribonuclease III domain; 2.
DR HAMAP; MF_00104; RNase_III; 1.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR011907; RNase_III.
DR InterPro; IPR000999; RNase_III_dom.
DR InterPro; IPR044442; RNAse_III_DSRM__animal.
DR InterPro; IPR036389; RNase_III_sf.
DR PANTHER; PTHR11207:SF0; RIBONUCLEASE 3; 1.
DR PANTHER; PTHR11207; RIBONUCLEASE III; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF14622; Ribonucleas_3_3; 1.
DR Pfam; PF00636; Ribonuclease_3; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00535; RIBOc; 2.
DR SUPFAM; SSF54768; dsRNA-binding domain-like; 1.
DR SUPFAM; SSF69065; RNase III domain-like; 2.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS00517; RNASE_3_1; 2.
DR PROSITE; PS50142; RNASE_3_2; 2.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000001075};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00266}.
FT DOMAIN 874..1054
FT /note="RNase III"
FT /evidence="ECO:0000259|PROSITE:PS50142"
FT DOMAIN 1105..1231
FT /note="RNase III"
FT /evidence="ECO:0000259|PROSITE:PS50142"
FT DOMAIN 1258..1332
FT /note="DRBM"
FT /evidence="ECO:0000259|PROSITE:PS50137"
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 445..495
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..103
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..163
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 351..393
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 445..476
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 477..491
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1372 AA; 158932 MW; 61F832563B5D1436 CRC64;
MQGNTCHRMS FHPGRGCPRG RGGHGARPSA PSFRPQNLRL LHPQQPPVQY QYEPPSAPST
TFSNSQASNF MPPRPDFVPY PPPMPPSSQG PAPPCPARPP YSNHQMRHPF PVPPCFPPMP
PPMPCPNNPP VSGAPPGQGT FPFMVPPPSM PHPPPPPVMP QQVNYQYPPG YSHNFPPPNF
NSFQNNSSSF PPSANNSSSP HFRHLPPYSL PKAQSERRSP ERLKHYDDHR HRDHSHGRGE
RHRSLERRER GRSPERRRPD SRYRSEYERG RTPSRHRSYE RSRERERERH RHRENRRSPS
PERSYKKEYK RSGRSYGLPV APEPAGCTPE SPGEITKNTD SWVPPPETVN HRSPSREKKR
ARWEEEKDRW SDSQSSGKEK SYTSIKEKEP EEAPPDRNEE EEEELLKPVW IRCTHSESYY
SSDPMDQVGD STVVGTSRLR DLYDKFEEEL GNRQEKAKAA RPPWEPPKTK LDEDLESSSE
SECESDEEST CSSSSDSEVF DVIAEIKRKK AHPDRLHDEL WYNDPGQMND GPLCKCSAKA
RRTGIRHSIY PGEEAIKPCR PMTNNAGRLF HYRITVSPPT NFLTDRPTVI EYDDHEYIFE
GFSMFAHAPL TNIPLCKVIR FNIDYTIHFI EEMMPENFCV KGLELFSLFL FRDILELYDW
NLKGPLFEDS PPCCPRFHFM PRFVRFLPDG GKEVLSMHQI LLYLLRCSKA LVPEEEIANM
LQWEELEWQK YAEECKGMIV TNPGTKPSSV RIDQLDREQF NPDVITFPII VHFGIRPAQL
SYAGDPQYQK LWKSYVKLRH LLANSPKVKQ TDKQKLAQRE EALQKIRQKN TMRREVTVEL
SSQGFWKTGI RSDVCQHAMM LPVLTHHIRY HQCLMHLDKL IGYTFQDRCL LQLAMTHPSH
HLNFGMNPDH ARNSLSNCGI RQPKYGDRKV HHMHMRKKGI NTLINIMSRL GQDDPTPSRI
NHNERLEFLG DAVVEFLTSV HLYYLFPSLE EGGLATYRTA IVQNQHLAML AKKLELDRFM
LYAHGPDLCR ESDLRHAMAN CFEALIGAVY LEGSLEEARQ LFGRLLFNDP DLREVWLHYP
LHPLQLQEPN TDRQLIETSP VLQKLTEFEE AIGVIFTHVR LLARAFTLRT VGFNHLTLGH
NQRMEFLGDS IMQLVATEYL FIHFPDHHEG HLTLLRSSLV NNRTQAKVAE ELGMQEYAIT
NDKTKRPVAL RTKTLADLLE SFIAALYIDK DLEYVHTFMN VCFFPRLKEF ILNQDWNDPK
SQLQQCCLTL RTEGKEPDIP LYKTLQTVGP SHARTYTVAV YFKGERIGCG KGPSIQQAEM
GAAMDALEKY NFPQMAHQKR FIERKYRQEL KEMRWEREHQ EREPDEAEDV KK
//
