ID G3HEV2_CRIGR Unreviewed; 544 AA.
AC G3HEV2;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 24-JAN-2024, entry version 56.
DE SubName: Full=Ras guanyl-releasing protein 3 {ECO:0000313|EMBL:EGW02700.1};
GN ORFNames=I79_009099 {ECO:0000313|EMBL:EGW02700.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:EGW02700.1, ECO:0000313|Proteomes:UP000001075};
RN [1] {ECO:0000313|Proteomes:UP000001075}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHO K1 cell line {ECO:0000313|Proteomes:UP000001075};
RX PubMed=21804562; DOI=10.1038/nbt.1932;
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan H.C., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.";
RL Nat. Biotechnol. 29:735-741(2011).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000256|ARBA:ARBA00004514}.
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DR EMBL; JH000321; EGW02700.1; -; Genomic_DNA.
DR AlphaFoldDB; G3HEV2; -.
DR STRING; 10029.G3HEV2; -.
DR eggNOG; KOG3417; Eukaryota.
DR InParanoid; G3HEV2; -.
DR Proteomes; UP000001075; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 2.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF178; RAS GUANYL-RELEASING PROTEIN 3; 1.
DR Pfam; PF00617; RasGEF; 2.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001075};
KW Zinc {ECO:0000256|ARBA:ARBA00022771};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 139..324
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT DOMAIN 342..377
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT REGION 442..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 494..530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..530
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 544 AA; 61933 MW; 05885178419970DF CRC64;
MGSSGLGKAA TLDELLNTCI EMFDDNGELN DSYLPRIVLL MHRWYLSSTE LAEKLLCIYL
FNCCVMYWIL KFPAEFNLDL GLIRMTEEFR EVASQLGYEK HINLMDISSI PSYDWMRRVT
QRKKASKKGK ACLLFDHLEP IELAEHLTFL EHKSFRRISF TDYQSYVIHG CLENNPTLER
SIALFNGISK WVQLMVLSKP SPQQRAEVIT KFINVAQNWN EMTELVSSNG NYCNYRKAFA
DCEGFKIPIL GVHLKDLIAV HVIFPDWLEE KKVNIVKMHQ LSVTLSELVS LQNASHHLEP
NMDLINLLTQ PTSPTTPNKP VVPLEWASGV IPKPDPTIIN KHIRKLVESV FRNYDHDHDG
YISQEDFESI AANFPFLDSF CVLDKDHQSP MALYSEVLPV CVQNEVFEFP GVAAGHRDLD
SRAITLVTGS SRKISVRLQR ATTSQATQTE PVWSEADWGD SGSHTFPKMK SKFHDKAAKD
KGFAKWENEK PMVQGGVDVV DRGTAFEPDQ DEGQDEAKQD GEVSAGKTQL ESERRRLRVL
MWAS
//
