ID G3IIW2_CRIGR Unreviewed; 600 AA.
AC G3IIW2;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=DNA polymerase alpha subunit B {ECO:0000256|ARBA:ARBA00018596, ECO:0000256|PIRNR:PIRNR018300};
GN Name=Pola2 {ECO:0000313|Ensembl:ENSCGRP00001013376.1,
GN ECO:0000313|RefSeq:XP_027264340.1};
GN ORFNames=I79_023790 {ECO:0000313|EMBL:EGW14747.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:EGW14747.1, ECO:0000313|Proteomes:UP000001075};
RN [1] {ECO:0000313|Proteomes:UP000001075}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHO K1 cell line {ECO:0000313|Proteomes:UP000001075};
RX PubMed=21804562; DOI=10.1038/nbt.1932;
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan H.C., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.";
RL Nat. Biotechnol. 29:735-741(2011).
RN [2] {ECO:0000313|EMBL:EGW14747.1}
RP NUCLEOTIDE SEQUENCE.
RA Xu X., Nagarajan H., Lewis N.E., Pan S., Cai Z., Liu X., Chen W., Xie M.,
RA Wang W., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W.,
RA Fan C.H., Wang J., Gui Y., Lee K.H., Betenbaugh M.J., Quake S.R.,
RA Famili I., Palsson B.O., Wang J.;
RT "The genomic sequence of the Chinese hamster ovary CHO-K1 cell line.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Ensembl:ENSCGRP00001013376.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
RN [4] {ECO:0000313|RefSeq:XP_027264340.1}
RP IDENTIFICATION.
RC STRAIN=17A/GY {ECO:0000313|RefSeq:XP_027264340.1};
RC TISSUE=Liver {ECO:0000313|RefSeq:XP_027264340.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Accessory subunit of the DNA polymerase alpha complex (also
CC known as the alpha DNA polymerase-primase complex) which plays an
CC essential role in the initiation of DNA synthesis.
CC {ECO:0000256|PIRNR:PIRNR018300}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|PIRNR:PIRNR018300}.
CC -!- SIMILARITY: Belongs to the DNA polymerase alpha subunit B family.
CC {ECO:0000256|ARBA:ARBA00007299, ECO:0000256|PIRNR:PIRNR018300}.
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DR EMBL; JH003119; EGW14747.1; -; Genomic_DNA.
DR RefSeq; XP_003514589.1; XM_003514541.3.
DR RefSeq; XP_007608850.1; XM_007610660.2.
DR RefSeq; XP_027264340.1; XM_027408539.2.
DR STRING; 10029.G3IIW2; -.
DR PaxDb; 10029-XP_007608850-1; -.
DR Ensembl; ENSCGRT00001017613.1; ENSCGRP00001013376.1; ENSCGRG00001014528.1.
DR GeneID; 100768307; -.
DR KEGG; cge:100768307; -.
DR CTD; 23649; -.
DR eggNOG; KOG1625; Eukaryota.
DR GeneTree; ENSGT00390000016784; -.
DR OMA; PFLDIEH; -.
DR OrthoDB; 49016at2759; -.
DR Proteomes; UP000001075; Unassembled WGS sequence.
DR Proteomes; UP000694386; Unplaced.
DR Proteomes; UP001108280; Chromosome 3.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0006270; P:DNA replication initiation; IEA:Ensembl.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:Ensembl.
DR GO; GO:0006606; P:protein import into nucleus; IEA:Ensembl.
DR Gene3D; 3.60.21.60; -; 2.
DR Gene3D; 1.10.8.530; DNA polymerase alpha-primase, subunit B, N-terminal domain; 1.
DR InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR InterPro; IPR043034; DNA_pol_alpha_B_N_sf.
DR InterPro; IPR016722; DNA_pol_alpha_bsu.
DR InterPro; IPR013627; Pol_alpha_B_N.
DR PANTHER; PTHR23061; DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT; 1.
DR PANTHER; PTHR23061:SF12; DNA POLYMERASE ALPHA SUBUNIT B; 1.
DR Pfam; PF04042; DNA_pol_E_B; 1.
DR Pfam; PF08418; Pol_alpha_B_N; 1.
DR PIRSF; PIRSF018300; DNA_pol_alph_2; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|PIRNR:PIRNR018300}; Nucleus {ECO:0000256|PIRNR:PIRNR018300};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000001075}.
FT DOMAIN 23..239
FT /note="DNA polymerase alpha subunit B N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08418"
FT DOMAIN 342..549
FT /note="DNA polymerase alpha/delta/epsilon subunit B"
FT /evidence="ECO:0000259|Pfam:PF04042"
FT REGION 105..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 600 AA; 66719 MW; 7761573420CF42C1 CRC64;
MAVSTQQLAE ELQIFGLDCE DALMEKLAEL CVLYRQTEEG MVSELIAFCT STGKVCLTAE
ILNSFEHEVL NKKLSKAWHN ASKDSGHAGA RDIVSIQELI EAEEEEETLL NSYTTPSKGP
HKRSSSTPET PLTKRSVSTR SPHQLLSPSS FSPSAAPSQK YSSRTNRGEV VTTFGSAQGV
SWSGRGGSAS VSLKVIGYPE PLTSSYKTMF QQLSDVREVL TCKIEELGSE LKEHYKIEAF
TPLLEPAQEP VILLGQIGCD SNGKLNSKSV ILEGDREHSS GTQIPVDLSD LKEYSLFPGQ
VVIMEGFNTT GRRFTATKLY EGVPLPFYQP TEEDADFEQT MVLVACGPYT TSDSITYDPL
LDLITIINHD RPDVCILFGP FLDAKHEQVE NCQLTSPFED VFKQCLRTVI EGTRSSGSHL
VFVPSLRDVQ HEPVYPQPPF TFSELPREDK KRVQFVSEPC SLSINGVIFG LTSTDLLFHI
GAEEICSSSG TPDRFSRILK HILTQRSYYP LYPPHEDMSI DYENFYTYAQ MPVTPDVFIV
PSELRYFVKD ILGCVCMNPG RLTKGQVGGT YGRLYFRRQP RAMDSKGRQG LSVAAQVVRI
//
