ID G3MZ03_BOVIN Unreviewed; 355 AA.
AC G3MZ03;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 2.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Lactosylceramide 4-alpha-galactosyltransferase {ECO:0000256|ARBA:ARBA00040835};
DE EC=2.4.1.228 {ECO:0000256|ARBA:ARBA00039051};
DE AltName: Full=Alpha-1,4-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00043186};
DE AltName: Full=Alpha-1,4-galactosyltransferase {ECO:0000256|ARBA:ARBA00041849};
DE AltName: Full=Globotriaosylceramide synthase {ECO:0000256|ARBA:ARBA00043154};
DE AltName: Full=UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase {ECO:0000256|ARBA:ARBA00041556};
GN Name=A4GALT {ECO:0000313|Ensembl:ENSBTAP00000054793.2,
GN ECO:0000313|VGNC:VGNC:49133};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000054793.2, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000054793.2, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000054793.2,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000054793.2}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000054793.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-
CC alpha-D-galactose = a globoside Gb3Cer (d18:1(4E)) + H(+) + UDP;
CC Xref=Rhea:RHEA:11924, ChEBI:CHEBI:15378, ChEBI:CHEBI:17950,
CC ChEBI:CHEBI:18313, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914;
CC EC=2.4.1.228; Evidence={ECO:0000256|ARBA:ARBA00043770};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11925;
CC Evidence={ECO:0000256|ARBA:ARBA00043770};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-Gal-(1<->1')-Cer + UDP-alpha-D-galactose = alpha-D-Gal-
CC (1->4)-beta-D-Gal-(1<->1')-Cer + H(+) + UDP; Xref=Rhea:RHEA:60044,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914,
CC ChEBI:CHEBI:143593, ChEBI:CHEBI:143594;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60045;
CC Evidence={ECO:0000256|ARBA:ARBA00036489};
CC -!- PATHWAY: Glycolipid biosynthesis. {ECO:0000256|ARBA:ARBA00004934}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
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DR RefSeq; XP_015319105.1; XM_015463619.1.
DR RefSeq; XP_015319106.1; XM_015463620.1.
DR RefSeq; XP_015319107.1; XM_015463621.1.
DR RefSeq; XP_015319108.1; XM_015463622.1.
DR RefSeq; XP_015319109.1; XM_015463623.1.
DR RefSeq; XP_015319110.1; XM_015463624.1.
DR RefSeq; XP_015319111.1; XM_015463625.1.
DR RefSeq; XP_015326918.1; XM_015471432.1.
DR RefSeq; XP_015326919.1; XM_015471433.1.
DR RefSeq; XP_015326920.1; XM_015471434.1.
DR RefSeq; XP_015326921.1; XM_015471435.1.
DR RefSeq; XP_015326922.1; XM_015471436.1.
DR RefSeq; XP_015326923.1; XM_015471437.1.
DR RefSeq; XP_015326924.1; XM_015471438.1.
DR AlphaFoldDB; G3MZ03; -.
DR SMR; G3MZ03; -.
DR STRING; 9913.ENSBTAP00000054793; -.
DR PaxDb; 9913-ENSBTAP00000054793; -.
DR Ensembl; ENSBTAT00000037673.4; ENSBTAP00000054793.2; ENSBTAG00000026497.4.
DR GeneID; 618369; -.
DR CTD; 53947; -.
DR VEuPathDB; HostDB:ENSBTAG00000026497; -.
DR VGNC; VGNC:49133; A4GALT.
DR eggNOG; KOG1928; Eukaryota.
DR GeneTree; ENSGT00510000047981; -.
DR HOGENOM; CLU_049512_2_0_1; -.
DR InParanoid; G3MZ03; -.
DR OMA; CKDSYVV; -.
DR OrthoDB; 2235518at2759; -.
DR TreeFam; TF324053; -.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000026497; Expressed in granulosa cell and 107 other cell types or tissues.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008375; F:acetylglucosaminyltransferase activity; IBA:GO_Central.
DR GO; GO:0008378; F:galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006493; P:protein O-linked glycosylation; IBA:GO_Central.
DR Gene3D; 3.90.550.20; -; 1.
DR InterPro; IPR007652; A1-4-GlycosylTfrase_dom.
DR InterPro; IPR007577; GlycoTrfase_DXD_sugar-bd_CS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR12042:SF17; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE; 1.
DR PANTHER; PTHR12042; LACTOSYLCERAMIDE 4-ALPHA-GALACTOSYLTRANSFERASE ALPHA- 1,4-GALACTOSYLTRANSFERASE; 1.
DR Pfam; PF04572; Gb3_synth; 1.
DR Pfam; PF04488; Gly_transf_sug; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 4: Predicted;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Transferase {ECO:0000256|ARBA:ARBA00022676};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..43
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 224..351
FT /note="Alpha 1,4-glycosyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04572"
SQ SEQUENCE 355 AA; 40271 MW; A4E0F270CB7C3325 CRC64;
MTSAPPDCLL RLLRDTPRQR VCTLFIIGFK LTFFVSVMVY WHVVGEPGGQ GQLSNLPVDV
PCPHMVPPTP PPSGIPPPGN IFFLETSDRT NPNFLFMCSV ESAARAHPES PVVVLMKGLP
GGNASRPRHL GLSLLGCFPN VQMRPLDLGE LFRDTPLAAW YAAVQRRWEP YLLPVLSDAS
RIALLWKFGG IYLDTDFIVL KDLRNLTNAL GTQSRYVLNG AFLAFEQHHE FMAQCMRDFV
THYNGWIWGH QGPQLLTRVF KKWCSIRSLS ESRACRGVTT LPPEAFYPIP WQNWKKYFED
ISPQELTRLL NATFAVHVWN KKSQGTRFEA TSKALLAQLH ARYCPTTHKA MKMYL
//
