ID G3NB94_GASAC Unreviewed; 4081 AA.
AC G3NB94;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 72.
DE RecName: Full=DNA-dependent protein kinase catalytic subunit {ECO:0000256|ARBA:ARBA00018077};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN Name=PRKDC {ECO:0000313|Ensembl:ENSGACP00000002590.2};
OS Gasterosteus aculeatus (Three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000002590.2, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000002590.2, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSGACP00000002590.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|ARBA:ARBA00011031}.
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DR Ensembl; ENSGACT00000002599.2; ENSGACP00000002590.2; ENSGACG00000001974.2.
DR GeneTree; ENSGT00940000155633; -.
DR Proteomes; UP000007635; Unassembled WGS sequence.
DR Bgee; ENSGACG00000001974; Expressed in head kidney and 9 other cell types or tissues.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004677; F:DNA-dependent protein kinase activity; IEA:InterPro.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05172; PIKKc_DNA-PK; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR037706; DNA-PK_dom.
DR InterPro; IPR046804; DNA-PKcs_N.
DR InterPro; IPR046803; DNAPKcs_CC1-2.
DR InterPro; IPR012582; DNAPKcs_CC3.
DR InterPro; IPR045581; DNAPKcs_CC5.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF68; DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT; 1.
DR Pfam; PF20500; DNA-PKcs_N; 1.
DR Pfam; PF20502; DNAPKcs_CC1-2; 1.
DR Pfam; PF08163; DNAPKcs_CC3; 1.
DR Pfam; PF19704; DNAPKcs_CC5; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM01344; NUC194; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2868..3520
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 3704..4006
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 4049..4081
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 2050..2078
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2727..2754
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 4081 AA; 462730 MW; DCFFB3D6B6A1E919 CRC64;
SEGSAAGGVH GLLLKLHNSL SDEDTRAAAL KCHDIVGDLG QECMLTPTEN ELALQMSLLF
SKDYGLLGFL RKSLGSEELR DTRVDILVFL EKFLDRISPR VKGWERTCAN DIRDTCMVVY
TKDKTAKCRT LVLELLIKVL QTTKASSVVA ELRICELFNR YYGELCQKSK LPDSVLGKIY
ELLGVLAEVH PSEMSSNSDK LYKAYLGELK EQMTSLTKEP KLFVVAGCLR GLASLMVNFT
KTMDEDPATS KEIFQYALKA ISPQMSMTRY AVIFAGLRLL AKHASQFSSC LMDHYRALFE
VMSSLCGHIN SEMKKTSYHA LEAFLKQVAM VVAENIEEHK GKLKFFMQKF CGIIRNMDST
HKELSIAIRG YGFFATPCKK VCPQDVDLMY TELIQRCKQM YLTESDGEDE SFYQLPSFLD
SIASVLIHLD KIPEVYTPLL ERLLVVQIDS FPQYSEKMQT ACCRSILKVL VAMASKGPVL
WGFISSVVHQ GLIRVCSKPA LLSEDKDNGR SESPQLHTGK WKVPSSHNYL ALFKSLLDCD
RLKDSGFLDG AFESQNAALV SLSRLLYDEL VKSILKIVEK LDLSVQKVNT GGEVRSPHQA
LDDAAHVLPS SDPTAHLLPN KIKDFTAFIN LVDFCSELLL TKHLEYFQFW MYCLSHELIL
HSIRNPLVSG FYKLLSVTMK VANRIKFYQG VCPRSSTSPQ SDTGKSACFA LFSKFGKEVC
VRMKQYKDEL LASCLTFVLS LHYNMVALDL KAYCPALEAA LKLGLSHVPL ANTALDALED
WSSHIPLDTM QHCYTSILPL LDGYLKTSSD GENHDDSNWE VMASLSSRSD KGYSRVMTRL
LKKSKQLPTE DAPLVAVRLR VVKLLGHLGG KLNRNLVTGV SSEETMKKFV AWDSEKRLSF
AVPFADMKPV IYLDPFLPHI SELALSTSDR QTKVAACELL HSLVVYMVGK SAQMVEGDNS
LPPMYKLHKR LFPVLLRLCD VDQVTRQLFE PLVMQLIHWF TNNKKFESKD TVAILEAIMD
GVVDPMDSTL RDFCGRCIEE FVKWSIKQTT PKQQEKSPTN MKSLFKRIYS LALHPNGFKR
LGAALAFNSI YRQFREEASL VEQFIFEVLV IFVESLALAH SDEPSMGTLQ QCSSAIDHVK
RIINQKAPSL NRHNTKIRVP RGFPPDEKLC LSDMVLWLLE QCGRPQTACR HKCMYLFYEF
IPLLPGKESP SQWVDGKLKD HGKGFLISRF EGGGLFSQPT LRGLTVPSSV RATLQWMDVL
LAALDCYNTF IQLHIIKPPL MLSTREKSSF LKAICFFLTE LATGNLAAAE SCFSHGQKTS
HFSPREVEQY NFSKCTIIVR LLEFVTRILV KGDKEFWKLL EQDIFVPNLF ELTALVVCEP
STVGFNMADV EVMRKLPEVC VPLLKALLAS PYRTRLEGSL RTKISRKSVE ELCTVDFYHS
STQRHHDQME MVLSSCKQIH KADFLSSILH SQDGACAKSI GSRLLMTVYK GIAPGENRNA
LPSLDIITKS LADGLLQLAF SLNQQSEPLV DLLLNTILLT VPISGSHCHN VLSFSHGEYF
YSLFQMTINT ELLKDLNTTV PCLMLASSQN PSMVSVLLNG MLDHSFRERS VRKTQGKQLV
EEVLRRWNSL QSWWKGHSST PESKTATLLL LSKLLQIDSS VCSDTNHEAF RPVFSTFTML
LVDMDLPLNL KSQALLVLPF FTTLPEEPLG ELQEALDALV ASHFPMQSDE FAKGSLHRNN
YMDCIRKLLD ALELSQSPLL LKLMAGILCR DRKHIMEEPF QTCFQRIARR SSSERQLQLL
CTVYRGVQQG DVPMSSMLQA AMERVLLPLA SHCSTKALND FFVANVSDMM VVLLSRFTKS
NEMEFEIQLM KKSGCYKLME LLYSQLPKEE VYSKESRINQ AYCCSDKTEG NELSKTLIKL
CFDAFTENMA GETQLLELRR QYRCAAYNCA IAAISCSFSE PKFYQGFLFS EKPEKNQFIL
ENIIDVERTY NFPIEIEVPL ERKKKYVMIR KEVSEENGEA PVYLSSQSYM ADSSLSEEMS
QFDFSTGVQS FSFSSQNPEG PRRTVAKRTE ETNPSQDAMV DLEMDELNQH ECMTTMTALI
GHMLRNNITP KIEQGNVPGE LPPWMKFLHG KLSNPATPLN IRLFISKLII NTEEVFRPYA
KHWLGPLLQL VVSGNNGGEG IHFMVVEIIV TVLSWTSLAS PKGNPRDEVL ANRLLEFLMK
HCFHSKRAVF RHNLEIIRTL VECWKDCLHV PYRYLIHERF CGNDPNSKDN SVGLQLLGII
LGNNLPPYDA SCGIEYDRYI QSLTNNVSFV RYKDVYAAAA EIIGLILKNM TETGLLSLAA
SKITNLKKKD MDDKFIICLN KVSKHFPPFM DRFVNYVFSL LPKLHGILKT HCLECVLSRA
DVIPDIFLHL KTTGFIQMMG HRDEARQRVC LDIIHKIVAC LTPAQLQEIL GAVISFVSHP
SPVCRERMYD VLMWIQDNYS DAESMEDNTS VEVLNSAKEA LLQGLSEENQ GLQLYVRNFW
SQEKRLPTTT LERMLTVLSS LYSCQIEKSF LSLATNLLLE RTSQSPDFIR NIFEYPLSEC
TFQDYVIDSN WRFRSTVMTP MFVETQSSQG PESVMASQSG GIKGKLRATQ SSLEFSQTQT
TGRRTAYNWL TGSSVDTLAE YTLSSESLSS LLAFDKKPAR QNAVRRPVGE GFGLRRLTAS
TDEVDSRTRA ANEQRNNILR LRRRFIKDQE KVSLNFAQKE IQQQRQKKVD KADQRLRKEA
QVTLYRNYRA GDFPDIQIPY SSLIAPLQAL AQRDPILAKQ LFSALFDGIL QEMETHKARG
ESGRIKEELR RCMNTFLSKS TVCFPPFIAC IQDMCHQHKE LHHLDPAAIS STCLMSLQQP
SGILLLEEGL LHSGGQEEPA AKRSRGRKEI PPDTNKWIHL ARLYRSLEDY DVVRGIFGGK
VGTRSITCAA LQAEANSDFA EAVKLYNEAL NTEHWTDGEP TESEKDFWEI AAMDAYSHLT
EWKSLRYCST LYVDMMWSEP LYQEMYLRHM IRSMLKQLQL GEADQTLLSF IDAAMAVEER
KKLLESHYSQ ELSLLYILQE DYDRATYYTN YAMQLFMQNY SSVDTLLTQS RLTILQSVQA
LTEIQDFLQF IKGEGEQSLS MRSLTRLIGL WAERYPDAKA DPVNLWDDII TSRCFFLDKI
RIKLGGHAPS DSMEVDGSED QAGAIDTLVN DCKFSMKLQM ADSALKQNNF PVATKMLKEL
HKEAKTNRGW LLRWVHGFSR YNHKRSQTQG PVEQISAMLK TIPFLGKQGG KDMYSSATAK
VFRCQKILEG KSFHIMATAV GRSPSVLGTM EAEKAGRVAQ LSGAAWPDEN AKKVEVGLQL
KALNLLCDAT RKADEELQSY TQECVESSSI IETYMTLANF CDKLLRGAEQ SDTENQFPNM
LALPVHILEN LLKALKMNSE EARLKFPRLL QIIELYPSET LDLMMKEMMS VPCWMLIGWI
SQMVALLDKP EAVAVQRCVE QIADCYPQAL VYALMISSES YQFEDSATGH QQRGFVDRLN
SVLDKEGPVK NFVNSLQQLT NPEMLFRDWW DCMKNELDKP GFDEKRMGRM YEEMHSSLGN
CNTGGHGMFR ARFIRKFAKD VEKLLGSNGS KLFQKRKDKS FLKQVDELAA SMRGFQKEPG
NLKEYSPWLS GFKADTFSNE LEIPGQYDGR SKPLPEYHAK ITGFDERVMV MSSIRRPKRL
IIRGDDERDH PFLVKGGEDL RQDQRIEQLF AVMNIMLSHD AACTHRGMQL RTYQVIPITT
RCLCAEYHNS WLQKFAVSKK DPNLFSLYGK AYEKAARNET VTYFLKALQC VPSDLLKRAF
LKMCNSPEAF LSLRSHFIGS HALLCVSQWI LGIGDRHLSN FMINMETGGM IGIDFGHAFG
SATQFLCVPE LMPFRLTQQF VNLMQPLRES GLIQSIMVHS LRAYSAEPDL LLNTMDVFVK
EPSLDWKNFE LKQMKKGGTW TDSVNTKEIN WYPLQKVDFA RRKLEGANPA AITSEELKLG
FEKDPAFQGM QSVALGSAEH NIRARLPLAG LSVEKQVDCL LDQAMDPNVL GRVWVGWEPW
F
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