ID   G3P191_GASAC            Unreviewed;       322 AA.
AC   G3P191;
DT   16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT   16-NOV-2011, sequence version 1.
DT   27-MAR-2024, entry version 57.
DE   RecName: Full=sphingolipid 4-desaturase {ECO:0000256|ARBA:ARBA00012021};
DE            EC=1.14.19.17 {ECO:0000256|ARBA:ARBA00012021};
OS   Gasterosteus aculeatus (Three-spined stickleback).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC   Gasterosteus.
OX   NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000011364.1, ECO:0000313|Proteomes:UP000007635};
RN   [1] {ECO:0000313|Ensembl:ENSGACP00000011364.1, ECO:0000313|Proteomes:UP000007635}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSGACP00000011364.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|PIRNR:PIRNR017228}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the fatty acid desaturase type 1 family. DEGS
CC       subfamily. {ECO:0000256|ARBA:ARBA00006146,
CC       ECO:0000256|PIRNR:PIRNR017228}.
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DR   AlphaFoldDB; G3P191; -.
DR   STRING; 69293.ENSGACP00000011364; -.
DR   Ensembl; ENSGACT00000011387.1; ENSGACP00000011364.1; ENSGACG00000008597.1.
DR   eggNOG; KOG2987; Eukaryota.
DR   GeneTree; ENSGT00390000013448; -.
DR   InParanoid; G3P191; -.
DR   OMA; FEWVYND; -.
DR   TreeFam; TF313582; -.
DR   Proteomes; UP000007635; Unassembled WGS sequence.
DR   Bgee; ENSGACG00000008597; Expressed in pharyngeal gill and 5 other cell types or tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042284; F:sphingolipid delta-4 desaturase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030148; P:sphingolipid biosynthetic process; IEA:InterPro.
DR   CDD; cd03508; Delta4-sphingolipid-FADS-like; 1.
DR   InterPro; IPR011388; DES1/DES2.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR013866; Sphingolipid_d4-desaturase_N.
DR   PANTHER; PTHR12879; SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2; 1.
DR   PANTHER; PTHR12879:SF21; SPHINGOLIPID DELTA(4)-DESATURASE_C4-MONOOXYGENASE DES2; 1.
DR   Pfam; PF00487; FA_desaturase; 1.
DR   Pfam; PF08557; Lipid_DES; 1.
DR   PIRSF; PIRSF017228; Sphnglp_dlt4_des; 1.
DR   SMART; SM01269; Lipid_DES; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum {ECO:0000256|PIRNR:PIRNR017228};
KW   Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516,
KW   ECO:0000256|PIRNR:PIRNR017228};
KW   Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW   ECO:0000256|PIRNR:PIRNR017228};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR017228};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|PIRNR:PIRNR017228};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        43..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        210..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          5..43
FT                   /note="Sphingolipid delta4-desaturase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM01269"
SQ   SEQUENCE   322 AA;  37290 MW;  C381CBEE43E96B10 CRC64;
     MGKTGGRGDF EWVYNDQPHT SRRKVILAKY PEIKSLMGPD PQLKWVVSAM VLTQLLACYL
     VHDLSWKWIF FWAYAFGGCI NHSLTLAIHD ISHNVAFGNK LAKWNRWFAM WANLPIGLPY
     SASFKKYHID HHRYLGGDQL DVDIPTDIEG WFFCTPARKV LWLFLQPLFY ALRPLIVNPK
     PLGQLEILNA AVQLTADLII YHLWGIKPIV YLIAGSILCM GLHPISGHFI AEHYMFLKGH
     ETYSYYGSLN LITFNVGYHM EHHDFPSIPG SKLPQVKHIA AEYYNNLPQH SSWIRVLWDF
     VFDDSIGPYA RIKREYKLSK QQ
//