ID G3PKL0_GASAC Unreviewed; 1263 AA.
AC G3PKL0;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 68.
DE RecName: Full=mitogen-activated protein kinase kinase kinase {ECO:0000256|ARBA:ARBA00012406};
DE EC=2.7.11.25 {ECO:0000256|ARBA:ARBA00012406};
GN Name=MAP3K6 {ECO:0000313|Ensembl:ENSGACP00000018140.1};
OS Gasterosteus aculeatus (Three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000018140.1, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000018140.1, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSGACP00000018140.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000478};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.25; Evidence={ECO:0000256|ARBA:ARBA00000106};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC protein kinase family. MAP kinase kinase kinase subfamily.
CC {ECO:0000256|ARBA:ARBA00006529}.
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DR AlphaFoldDB; G3PKL0; -.
DR Ensembl; ENSGACT00000018175.1; ENSGACP00000018140.1; ENSGACG00000013722.1.
DR GeneTree; ENSGT00940000159398; -.
DR Proteomes; UP000007635; Unassembled WGS sequence.
DR Bgee; ENSGACG00000013722; Expressed in mesonephros and 5 other cell types or tissues.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004709; F:MAP kinase kinase kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd06624; STKc_ASK; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR046872; DRHyd-ASK.
DR InterPro; IPR046873; HisK-N-like.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR043969; MAP3K_PH.
DR InterPro; IPR025136; MAP3K_TRAF-bd.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR11584:SF391; MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 6; 1.
DR PANTHER; PTHR11584; SERINE/THREONINE PROTEIN KINASE; 1.
DR Pfam; PF19039; ASK_PH; 1.
DR Pfam; PF20309; DRHyd-ASK; 1.
DR Pfam; PF20302; HisK-N-like; 1.
DR Pfam; PF13281; MAP3K_TRAF_bd; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022527};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022527}.
FT DOMAIN 631..889
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REGION 883..929
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1104..1132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1134..1168
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 888..903
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1115..1130
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 660
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 1263 AA; 141097 MW; D0B3CC68D784F7EB CRC64;
LHDQRRKDPV RVSAGSLWQD ASAVDPSGAG KPLTSPRSRT RAVSVAYIVN EDASEPRGEE
NLTVDCLKEA CADAHALFKT ISFERISLGA TDILDSFYNA DVAVVEMSDS LCQPSLYYHL
GVRESFSMTN NIILYCYKQD SDVQALKRDD GAVRGSREQC GTYTFIPYVV SPQGKAFACD
ATIMMSGIKD LMQPSFQLEP LLTPLVERMV HLLNNVQVQS SEYFQESIRH EIRTARERFS
GRALSEELSR IQKRLDSVEL LTPDIVMNLL LSYRDIQDYD AIIKLVETLN DLPTCLVAKH
QNIKFTYIFA LNRRNKSGDR AKALEVILPI VESGVNVVSD IYCLCGRIYK DMFMSSGFTD
QGSRDQACYW YGKAFETEPT LHSGINNVVL LMVAGHEFDT SIELRKTGVT LSTLLGRKGS
LEKMKNYWDV GFYLGANILV NEHRKVTAAS EKLYRLQAPV WYVASIMETY ILYRQFAKLP
EVKSAKQDTM DFWMELLLQA CKPTDSTGRC PVLILEPSKV LQPAIVCVSE EDQSRTVQLE
HVTPLEKGLH QWTFPASAIR GVSASKIDER SCFLYVHFNS DDFQLCFPSE LHCKGFCELV
NSLLQLAQQP DRDQDPVAEG IPEYIYETND NGDKVVLGKG TYGVVYAGRD QSNHVRIAIK
EIPERDRTYS QPLHEEIALH KRLKHRNIVQ YLGSVSQNGF IKIFMEEVPG GSLSSLLRSK
WGPLKDNEAT ITFYTKQILD GLKYLHDNQI VHRDIKGDNV LINTYSGVLK ISDFGTSKRL
AGINPCTETF AGTLQYMAPE IIDQGPRGYG KPADIWSLGC TIIEMATGKP PFHELGSPQA
AMFKVGMFKI HPKVPECMSD EAKAFIMKCF VPEPDDRASS AELLSDPYLR PSHRKRAKAP
QESEPVDHVS AGGTSPSAGR MTTPGAPPAP AACCRESGCC PTGDQDLLVI PEDPSDMSSP
ASADENMGLF MLRKNSERRA TLHRVLTDYI SLVVSNILEH DRGTALTADN ITELISCLRD
NIHTPDRKQL SGRLQALRAA MRSAAVSPSS LQAVLFSFQD AVKKVLRQEQ VKPHWMFALD
NLLRQAVQDA ITVLLPGAAS SCPLKDDAEQ TASTCDVERP DSPADSSSSL SEKLRELRLE
TGRLLSQLNE KEREYQQLLR NSVQRKQEQI DALRCAPATE NSSEPEAKAL VDWLNSLPVD
QNTINKLLAH DFTLDCLLYV ASREDLMYCG IRCGMLCRIW AAISARRKTP LRTHNEDSED
TLL
//
