ID G3PYP3_GASAC Unreviewed; 508 AA.
AC G3PYP3;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Prenylcysteine oxidase 1 {ECO:0000256|ARBA:ARBA00040608};
DE EC=1.8.3.5 {ECO:0000256|ARBA:ARBA00039077};
OS Gasterosteus aculeatus (Three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000022733.1, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000022733.1, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSGACP00000022733.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + S-(2E,6E)-farnesyl-L-cysteine = (2E,6E)-farnesal +
CC H2O2 + L-cysteine; Xref=Rhea:RHEA:30231, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:15894, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:62141; EC=1.8.3.5;
CC Evidence={ECO:0000256|ARBA:ARBA00036078};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30232;
CC Evidence={ECO:0000256|ARBA:ARBA00036078};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(2E,6E,10E)-geranylgeranyl]-L-cysteine + H2O + O2 =
CC (2E,6E,10E)-geranylgeranial + H2O2 + L-cysteine;
CC Xref=Rhea:RHEA:70407, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:35235, ChEBI:CHEBI:189549,
CC ChEBI:CHEBI:189554; EC=1.8.3.5;
CC Evidence={ECO:0000256|ARBA:ARBA00036599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70408;
CC Evidence={ECO:0000256|ARBA:ARBA00036599};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an S-prenyl-L-cysteine + H2O + O2 = a prenal + H2O2 + L-
CC cysteine; Xref=Rhea:RHEA:53892, Rhea:RHEA-COMP:13675, Rhea:RHEA-
CC COMP:13676, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:137934, ChEBI:CHEBI:137935;
CC EC=1.8.3.5; Evidence={ECO:0000256|ARBA:ARBA00036029};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:53893;
CC Evidence={ECO:0000256|ARBA:ARBA00036029};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRNR:PIRNR036292};
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|ARBA:ARBA00004371}.
CC -!- SIMILARITY: Belongs to the prenylcysteine oxidase family.
CC {ECO:0000256|ARBA:ARBA00009967, ECO:0000256|PIRNR:PIRNR036292}.
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DR AlphaFoldDB; G3PYP3; -.
DR STRING; 69293.ENSGACP00000022733; -.
DR Ensembl; ENSGACT00000022776.1; ENSGACP00000022733.1; ENSGACG00000017209.1.
DR eggNOG; ENOG502QSHJ; Eukaryota.
DR GeneTree; ENSGT00390000011206; -.
DR InParanoid; G3PYP3; -.
DR OMA; QMWVESI; -.
DR TreeFam; TF329001; -.
DR Proteomes; UP000007635; Unassembled WGS sequence.
DR Bgee; ENSGACG00000017209; Expressed in intestinal epithelial cell and 11 other cell types or tissues.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0001735; F:prenylcysteine oxidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030328; P:prenylcysteine catabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR010795; Prenylcys_lyase.
DR InterPro; IPR017046; Prenylcysteine_Oxase.
DR PANTHER; PTHR15944; FARNESYLCYSTEINE LYASE; 1.
DR PANTHER; PTHR15944:SF3; PRENYLCYSTEINE OXIDASE 1; 1.
DR Pfam; PF13450; NAD_binding_8; 1.
DR Pfam; PF07156; Prenylcys_lyase; 1.
DR PIRSF; PIRSF036292; Prenylcysteine_oxidase; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|PIRNR:PIRNR036292};
KW Flavoprotein {ECO:0000256|PIRNR:PIRNR036292};
KW Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|PIRNR:PIRNR036292};
KW Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..508
FT /note="Prenylcysteine oxidase 1"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003450285"
FT DOMAIN 127..497
FT /note="Prenylcysteine lyase"
FT /evidence="ECO:0000259|Pfam:PF07156"
SQ SEQUENCE 508 AA; 56549 MW; 38F8FC6551F07BAC CRC64;
ADKYLCVLIG ALLLLGLWHT GRRCAAQELQ DQPKKIAVVG AGIGGTSAAF YLRQEFGPGV
KIDVFEPGAV GGRLATVKIG DYEYETGGAV IHPLNLHMKH FIEKLGIPPR KEVPSKMAIF
DGKELTFEES DWFIVNFLRM LWRFGFSFLR MHMWVESVLD KFMRIYQYQQ YGYSFSSVDK
LLHAMGGDGF LTLLNQTLEE AMMGQGFSQV FLNNIVTPIT RVNYGQSVRI NGLVGAVSLA
GADPGLWAVD GGNKRVCSGL LYHSKSELVP ARVTSISFKD RPSKTGTTTS FYEVNYVGES
GSAHSLYDIV VIATPLHRGK SDITFSGFSP PIPSHYPGRY HQTVSTLVHG LLNTSYLGSA
KPASEFTVSE ILTTDSKDSV INSLSSLDPV HVPPGYKRPP ASQSNVWKVF SPQPLSQEQL
RSMFLSWDSV SETPWLAYPS YRPPQRNTPP FILHERLYYL NAVEWAASAM EMSAISARNV
ALLAHHRWHR QVGNVDQEDL HTRLRSEL
//
