ID G3Q7Q4_GASAC Unreviewed; 530 AA.
AC G3Q7Q4;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=malate synthase {ECO:0000256|ARBA:ARBA00012636};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636};
OS Gasterosteus aculeatus (Three-spined stickleback).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Cottioidei; Gasterosteales; Gasterosteidae;
OC Gasterosteus.
OX NCBI_TaxID=69293 {ECO:0000313|Ensembl:ENSGACP00000025914.1, ECO:0000313|Proteomes:UP000007635};
RN [1] {ECO:0000313|Ensembl:ENSGACP00000025914.1, ECO:0000313|Proteomes:UP000007635}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Lindblad-Toh K., Mauceli E., Grabherr M., Chang J.L., Lander E.S.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSGACP00000025914.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699};
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DR AlphaFoldDB; G3Q7Q4; -.
DR Ensembl; ENSGACT00000025965.1; ENSGACP00000025914.1; ENSGACG00000019604.1.
DR GeneTree; ENSGT00390000002932; -.
DR Proteomes; UP000007635; Unassembled WGS sequence.
DR Bgee; ENSGACG00000019604; Expressed in liver and 2 other cell types or tissues.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF2; MALATE SYNTHASE; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
PE 4: Predicted;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435};
KW Reference proteome {ECO:0000313|Proteomes:UP000007635};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 157..404
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 412..527
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 161
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 444
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 530 AA; 60368 MW; 45CE8DB54D26A95A CRC64;
LSTARAGAGL VSAGLEEEYK SLFSTRSLLF LQELISTFDE EVDQVLQLRI SRKAHLDLTA
DLPSFLESTT HIRRDPLWRV LPVPPRLQRR HVDIGDLAPC DTQRFVKALQ SSAQGLQVDF
DDGNCPTYYN QIKGLHNVFK AVHNQFPNLP HISHAPVLML RPRAWNMVEH NLMVVAGKEA
PGPLFDFGLL MFHCGKTLFE NKSGPFFYLS KVESFMEARL WNNIFVWTQH KLGLPLGSIK
ATVLIENILA AFEMEEILYE LRDHSAGLNC GIWDYSASFV NKFGHRQDFL LPDRSKYVNM
EKRFLRSYMD LLVQTCHRRG ALATGGMAAL LLPPDPLSDP HSTVLATVTR LKLLEIKAGV
DGFMVYDLSL IEPMQKLFKL HTEGDNQLHQ LRHDVTVTPE DLLSMPLGGV TLYGLKYNIA
VGVLFVNAWL SGKGHFFYRG QVEDSATAEI SRSQVWQWIR HQTQLEDDSR VVSRRLVTEL
IMELTMELKA LCHSVRAEQR LHTAADMFLE VVLKRDFPEF ITTYLIQDHT
//
