ID G3T6Q8_LOXAF Unreviewed; 392 AA.
AC G3T6Q8;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=Protein-tyrosine sulfotransferase {ECO:0000256|RuleBase:RU365018};
DE EC=2.8.2.20 {ECO:0000256|RuleBase:RU365018};
GN Name=TPST2 {ECO:0000313|Ensembl:ENSLAFP00000009233.3};
OS Loxodonta africana (African elephant).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Afrotheria; Proboscidea; Elephantidae; Loxodonta.
OX NCBI_TaxID=9785 {ECO:0000313|Ensembl:ENSLAFP00000009233.3, ECO:0000313|Proteomes:UP000007646};
RN [1] {ECO:0000313|Ensembl:ENSLAFP00000009233.3, ECO:0000313|Proteomes:UP000007646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000009233.3,
RC ECO:0000313|Proteomes:UP000007646};
RA Di Palma F., Heiman D., Young S., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Loxodonta africana (African elephant).";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLAFP00000009233.3}
RP IDENTIFICATION.
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000009233.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic
CC motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as
CC cosubstrate. {ECO:0000256|ARBA:ARBA00003886,
CC ECO:0000256|RuleBase:RU365018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine
CC 3',5'-bisphosphate + H(+) + O-sulfo-L-tyrosine-[protein];
CC Xref=Rhea:RHEA:16801, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:11688,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:58339,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:65286; EC=2.8.2.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000411,
CC ECO:0000256|RuleBase:RU365018};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
CC -!- SIMILARITY: Belongs to the protein sulfotransferase family.
CC {ECO:0000256|ARBA:ARBA00009988, ECO:0000256|RuleBase:RU365018}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; G3T6Q8; -.
DR STRING; 9785.ENSLAFP00000009233; -.
DR Ensembl; ENSLAFT00000011036.3; ENSLAFP00000009233.3; ENSLAFG00000011039.3.
DR eggNOG; KOG3988; Eukaryota.
DR GeneTree; ENSGT00390000006030; -.
DR HOGENOM; CLU_046916_0_1_1; -.
DR InParanoid; G3T6Q8; -.
DR OMA; VMMDSNH; -.
DR TreeFam; TF312910; -.
DR Proteomes; UP000007646; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:Ensembl.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR GO; GO:0008476; F:protein-tyrosine sulfotransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026634; TPST-like.
DR PANTHER; PTHR12788; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR PANTHER; PTHR12788:SF6; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR Pfam; PF13469; Sulfotransfer_3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000007646};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365018};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
SQ SEQUENCE 392 AA; 43728 MW; 8BF5E0E6F5FD9895 CRC64;
LKQAPRLGLP HPWPSMRLSV RRLLLVAGCA LALVLAVQLG HQVLECRAVL AGPRSPWQAM
RPEQEELVMV GTNHVEYRYG KAMPLIFVGG VPRSGTTLMR AMLDAHPEVR CGEETRIIPR
VLAMRQAWSK SGREKLRLDE AGVTDEVLDA AMQAFILEVI AKHGEPARVL CNKDPFTLKS
SVYLSRLFPN SKFLLMVRDG RASVHSMITR KVTIAGFDLS SYRDCLTKWN KAIEVMYTQC
LEVGRDKCLP VYYEQLVLHP RRSLKLILDF LGIAWSDAVL HHEDLIGKPG GVSLSKIERS
TDQVIKPVNL EALSKWTGHI PGDVVRDMAQ IAPMLARLGY DPYANPPNYG NPDPIVINNT
HRVLKGDYKT PANLKGYFQV NQNSTSSHLG SS
//
