ID   G3T8B3_LOXAF            Unreviewed;       573 AA.
AC   G3T8B3;
DT   16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT   16-NOV-2011, sequence version 1.
DT   27-MAR-2024, entry version 51.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00013303};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE   AltName: Full=Acid beta-galactosidase {ECO:0000256|ARBA:ARBA00031635};
GN   Name=GLB1 {ECO:0000313|Ensembl:ENSLAFP00000009915.4};
OS   Loxodonta africana (African elephant).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Afrotheria; Proboscidea; Elephantidae; Loxodonta.
OX   NCBI_TaxID=9785 {ECO:0000313|Ensembl:ENSLAFP00000009915.4, ECO:0000313|Proteomes:UP000007646};
RN   [1] {ECO:0000313|Ensembl:ENSLAFP00000009915.4, ECO:0000313|Proteomes:UP000007646}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000009915.4,
RC   ECO:0000313|Proteomes:UP000007646};
RA   Di Palma F., Heiman D., Young S., Johnson J., Lander E.S., Lindblad-Toh K.;
RT   "The Genome Sequence of Loxodonta africana (African elephant).";
RL   Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSLAFP00000009915.4}
RP   IDENTIFICATION.
RC   STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000009915.4};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Cleaves beta-linked terminal galactosyl residues from
CC       gangliosides, glycoproteins, and glycosaminoglycans.
CC       {ECO:0000256|ARBA:ARBA00002691}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809}.
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DR   AlphaFoldDB; G3T8B3; -.
DR   Ensembl; ENSLAFT00000011864.4; ENSLAFP00000009915.4; ENSLAFG00000011863.4.
DR   GeneTree; ENSGT00950000182942; -.
DR   HOGENOM; CLU_007853_7_2_1; -.
DR   Proteomes; UP000007646; Unassembled WGS sequence.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR026283; B-gal_1-like.
DR   InterPro; IPR048912; BetaGal1-like_ABD1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF172; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21317; BetaGal_ABD_1; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PIRSF; PIRSF006336; B-gal; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007646}.
FT   DOMAIN          36..351
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          398..510
FT                   /note="Beta-galactosidase 1-like first all-beta"
FT                   /evidence="ECO:0000259|Pfam:PF21317"
FT   ACT_SITE        183
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT   ACT_SITE        263
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ   SEQUENCE   573 AA;  64357 MW;  826624DC23F5BF59 CRC64;
     VPTDTPICTQ TELQTLVVLS NASQRTFKID DSRKCFLKDG QPFRYISGSI HYHRVPRFYW
     KDRLLKMKMA GLNAIQTYIP WNFHEPLPGQ YQFSDDHDVE HFIQLTHEIG LLVILRPGPY
     ICAEWDMGGL PAWLLEKQSI VLRSSDPYYL AAVDKWLGVL LPKMKPLLYQ NGGPIITVQV
     ENEYGSYFTC DYDYLRFLQK CFHSHLGDDV LLFTTDGARE SLLQCGTLQG LYATVDFGPV
     SNITAAFQTQ RRTEPRGPLV NSEFYTGWLD HWGQPHSRVS TEAVTSALYN MLALGANVNL
     YMFTGGTNFA YWNGANTPYA AQPTSYDYDA PLTEAGDLTE KYFAVREIIR KFEKVPDGPI
     PPSTPKFAYG KVALKKLKTV GEALNILSPS GPIKSLYPLT FVQVKQYFGF VLYRTTLPKN
     CSDPTPLSAP LNGVRDRAYI SVNGVPQGIL ERNKVFVTNI TGKAGATLDL LVENMGRVNY
     GRYTNDSKGL ISNLTLGNSI LTNWLIFPLN TEDAVRSHLR GWGGHGNSYH TEPEPHSSSN
     FTLPAFYMGN FSIPSGIPDL PQDTFIQFPG WTK
//