ID G3TS82_LOXAF Unreviewed; 339 AA.
AC G3TS82;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=tRNA methyltransferase 10 homolog A {ECO:0000256|ARBA:ARBA00014673};
DE EC=2.1.1.221 {ECO:0000256|ARBA:ARBA00012797};
DE AltName: Full=RNA (guanine-9-)-methyltransferase domain-containing protein 2 {ECO:0000256|ARBA:ARBA00029801};
DE AltName: Full=tRNA (guanine(9)-N(1))-methyltransferase TRMT10A {ECO:0000256|ARBA:ARBA00032540};
GN Name=TRMT10A {ECO:0000313|Ensembl:ENSLAFP00000018440.1};
OS Loxodonta africana (African elephant).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Afrotheria; Proboscidea; Elephantidae; Loxodonta.
OX NCBI_TaxID=9785 {ECO:0000313|Ensembl:ENSLAFP00000018440.1, ECO:0000313|Proteomes:UP000007646};
RN [1] {ECO:0000313|Ensembl:ENSLAFP00000018440.1, ECO:0000313|Proteomes:UP000007646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000018440.1,
RC ECO:0000313|Proteomes:UP000007646};
RA Di Palma F., Heiman D., Young S., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Loxodonta africana (African elephant).";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLAFP00000018440.1}
RP IDENTIFICATION.
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000018440.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00000855};
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DR RefSeq; XP_003410506.1; XM_003410458.2.
DR RefSeq; XP_010588768.1; XM_010590466.1.
DR RefSeq; XP_010588769.1; XM_010590467.1.
DR RefSeq; XP_010588770.1; XM_010590468.1.
DR AlphaFoldDB; G3TS82; -.
DR SMR; G3TS82; -.
DR STRING; 9785.ENSLAFP00000018440; -.
DR Ensembl; ENSLAFT00000033317.1; ENSLAFP00000018440.1; ENSLAFG00000029276.1.
DR GeneID; 100659629; -.
DR KEGG; lav:100659629; -.
DR CTD; 93587; -.
DR eggNOG; KOG2967; Eukaryota.
DR GeneTree; ENSGT00530000063169; -.
DR HOGENOM; CLU_034384_7_0_1; -.
DR InParanoid; G3TS82; -.
DR OMA; FKKNDGW; -.
DR OrthoDB; 2908056at2759; -.
DR TreeFam; TF330972; -.
DR Proteomes; UP000007646; Unassembled WGS sequence.
DR GO; GO:0015629; C:actin cytoskeleton; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0052905; F:tRNA (guanosine(9)-N1)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:Ensembl.
DR GO; GO:0002939; P:tRNA N1-guanine methylation; IEA:Ensembl.
DR CDD; cd18101; Trm10euk_A; 1.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR016653; TRM10/TRM10A.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; TRNA (GUANINE-9-) METHYLTRANSFERASE; 1.
DR PANTHER; PTHR13563:SF13; TRNA METHYLTRANSFERASE 10 HOMOLOG A; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PIRSF; PIRSF016323; tRNA_m1G_mtfrase_met; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000007646};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 88..279
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000259|PROSITE:PS51675"
FT REGION 1..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 279..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..34
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..329
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 210
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR016323-1"
SQ SEQUENCE 339 AA; 39471 MW; F6EF8562A8518198 CRC64;
MSSEMLPAFT ETCNVERKQN LNENQEQSQK PGSGEGCEPI SKRQMKKLIK QKQWEEQREL
RKQKRKEKRK RKKLERQCQL ESNSDGNDRK RIRRDVVQST LRLIVDCSFD DLMVLKDIKK
LHKQIQRCYA ENRRALHPVQ FYLTSHGGQL KKNMDENDKG WVNWKDIHIK PEHYSELIKK
EDLVYLTSDS PNVLKELDES KAYVIGGLVD HNHHKGVTYK QASDYGIEHA QLPLGNFVKM
NSRKVLAVNH VFEIILEYLE TRDWQEAFFT ILPQRKGAVP TDKARESSTS DKKTASVEDG
LDSDSSEEEY SRNELDSAHE EEKQDKENST ESAVNSVSH
//
