ID G3TX10_LOXAF Unreviewed; 370 AA.
AC G3TX10;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=Protein-tyrosine sulfotransferase {ECO:0000256|ARBA:ARBA00013262, ECO:0000256|RuleBase:RU365018};
DE EC=2.8.2.20 {ECO:0000256|ARBA:ARBA00013262, ECO:0000256|RuleBase:RU365018};
GN Name=TPST1 {ECO:0000313|Ensembl:ENSLAFP00000020118.1};
OS Loxodonta africana (African elephant).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Afrotheria; Proboscidea; Elephantidae; Loxodonta.
OX NCBI_TaxID=9785 {ECO:0000313|Ensembl:ENSLAFP00000020118.1, ECO:0000313|Proteomes:UP000007646};
RN [1] {ECO:0000313|Ensembl:ENSLAFP00000020118.1, ECO:0000313|Proteomes:UP000007646}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000020118.1,
RC ECO:0000313|Proteomes:UP000007646};
RA Di Palma F., Heiman D., Young S., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Loxodonta africana (African elephant).";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLAFP00000020118.1}
RP IDENTIFICATION.
RC STRAIN=Isolate ISIS603380 {ECO:0000313|Ensembl:ENSLAFP00000020118.1};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic
CC motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as
CC cosubstrate. {ECO:0000256|ARBA:ARBA00003886,
CC ECO:0000256|RuleBase:RU365018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine
CC 3',5'-bisphosphate + H(+) + O-sulfo-L-tyrosine-[protein];
CC Xref=Rhea:RHEA:16801, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:11688,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:58339,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:65286; EC=2.8.2.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000411,
CC ECO:0000256|RuleBase:RU365018};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}.
CC -!- SIMILARITY: Belongs to the protein sulfotransferase family.
CC {ECO:0000256|ARBA:ARBA00009988, ECO:0000256|RuleBase:RU365018}.
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DR AlphaFoldDB; G3TX10; -.
DR STRING; 9785.ENSLAFP00000020118; -.
DR Ensembl; ENSLAFT00000035378.1; ENSLAFP00000020118.1; ENSLAFG00000029219.1.
DR eggNOG; KOG3988; Eukaryota.
DR GeneTree; ENSGT00390000006030; -.
DR InParanoid; G3TX10; -.
DR OMA; LNDFRQC; -.
DR TreeFam; TF312910; -.
DR Proteomes; UP000007646; Unassembled WGS sequence.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR GO; GO:0008476; F:protein-tyrosine sulfotransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026634; TPST-like.
DR PANTHER; PTHR12788:SF4; PROTEIN-TYROSINE SULFOTRANSFERASE 1; 1.
DR PANTHER; PTHR12788; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR Pfam; PF13469; Sulfotransfer_3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000007646};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365018}.
SQ SEQUENCE 370 AA; 42172 MW; 18CCDABF71A4965F CRC64;
MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PAKLESTRAT VRTALDVKAN
KTFVYHKDMP LIFIGGVPRS GTTLMRAMLD AHPDVRCGEE TRVIPRILAL KQMWSRSSKE
KIRLDEAGVT DEVLDSAMQA FLLEIIVKHG EPAPYLCNKD PFALKSLTYL ARLFPNAKFL
LMVRDGRASV HSMISRKVTI AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE
QLVLHPERWM RTLLKFLHIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS
KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KILENTRRVI SYSHIFKIFC
CRKLETQRTN
//
