ID G3VB36_SARHA Unreviewed; 665 AA.
AC G3VB36;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 2.
DT 27-MAR-2024, entry version 67.
DE RecName: Full=Arachidonate 12-lipoxygenase, 12S type {ECO:0008006|Google:ProtNLM};
GN Name=LOC100933073 {ECO:0000313|Ensembl:ENSSHAP00000000390.2};
OS Sarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Metatheria; Dasyuromorphia; Dasyuridae; Sarcophilus.
OX NCBI_TaxID=9305 {ECO:0000313|Ensembl:ENSSHAP00000000390.2, ECO:0000313|Proteomes:UP000007648};
RN [1] {ECO:0000313|Ensembl:ENSSHAP00000000390.2, ECO:0000313|Proteomes:UP000007648}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21709235; DOI=10.1073/pnas.1102838108;
RA Miller W., Hayes V.M., Ratan A., Petersen D.C., Wittekindt N.E., Miller J.,
RA Walenz B., Knight J., Qi J., Zhao F., Wang Q., Bedoya-Reina O.C.,
RA Katiyar N., Tomsho L.P., Kasson L.M., Hardie R.A., Woodbridge P.,
RA Tindall E.A., Bertelsen M.F., Dixon D., Pyecroft S., Helgen K.M.,
RA Lesk A.M., Pringle T.H., Patterson N., Zhang Y., Kreiss A., Woods G.M.,
RA Jones M.E., Schuster S.C.;
RT "Genetic diversity and population structure of the endangered marsupial
RT Sarcophilus harrisii (Tasmanian devil).";
RL Proc. Natl. Acad. Sci. U.S.A. 108:12348-12353(2011).
RN [2] {ECO:0000313|Ensembl:ENSSHAP00000000390.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601885-1};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000256|PIRSR:PIRSR601885-1};
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00152}.
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DR AlphaFoldDB; G3VB36; -.
DR STRING; 9305.ENSSHAP00000000390; -.
DR Ensembl; ENSSHAT00000000395.2; ENSSHAP00000000390.2; ENSSHAG00000000343.2.
DR eggNOG; ENOG502QQSP; Eukaryota.
DR GeneTree; ENSGT00940000155191; -.
DR HOGENOM; CLU_004282_3_3_1; -.
DR InParanoid; G3VB36; -.
DR TreeFam; TF105320; -.
DR Proteomes; UP000007648; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0042383; C:sarcolemma; IEA:Ensembl.
DR GO; GO:0004052; F:arachidonate 12(S)-lipoxygenase activity; IEA:Ensembl.
DR GO; GO:0050473; F:arachidonate 15-lipoxygenase activity; IEA:Ensembl.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016165; F:linoleate 13S-lipoxygenase activity; IEA:Ensembl.
DR GO; GO:0019369; P:arachidonic acid metabolic process; IEA:Ensembl.
DR GO; GO:0061436; P:establishment of skin barrier; IEA:Ensembl.
DR GO; GO:0019395; P:fatty acid oxidation; IEA:Ensembl.
DR GO; GO:1901751; P:leukotriene A4 metabolic process; IEA:Ensembl.
DR GO; GO:0043651; P:linoleic acid metabolic process; IEA:Ensembl.
DR GO; GO:2001303; P:lipoxin A4 biosynthetic process; IEA:Ensembl.
DR GO; GO:2001306; P:lipoxin B4 biosynthetic process; IEA:Ensembl.
DR GO; GO:0019372; P:lipoxygenase pathway; IEA:Ensembl.
DR GO; GO:0010656; P:negative regulation of muscle cell apoptotic process; IEA:Ensembl.
DR GO; GO:0090331; P:negative regulation of platelet aggregation; IEA:Ensembl.
DR Gene3D; 3.10.450.60; -; 1.
DR Gene3D; 2.60.60.20; PLAT/LH2 domain; 1.
DR InterPro; IPR000907; LipOase.
DR InterPro; IPR013819; LipOase_C.
DR InterPro; IPR036226; LipOase_C_sf.
DR InterPro; IPR020834; LipOase_CS.
DR InterPro; IPR001885; LipOase_mml.
DR InterPro; IPR001024; PLAT/LH2_dom.
DR InterPro; IPR036392; PLAT/LH2_dom_sf.
DR PANTHER; PTHR11771; LIPOXYGENASE; 1.
DR PANTHER; PTHR11771:SF4; POLYUNSATURATED FATTY ACID LIPOXYGENASE ALOX12; 1.
DR Pfam; PF00305; Lipoxygenase; 1.
DR Pfam; PF01477; PLAT; 1.
DR PRINTS; PR00087; LIPOXYGENASE.
DR PRINTS; PR00467; MAMLPOXGNASE.
DR SMART; SM00308; LH2; 1.
DR SUPFAM; SSF49723; Lipase/lipooxygenase domain (PLAT/LH2 domain); 1.
DR SUPFAM; SSF48484; Lipoxigenase; 1.
DR PROSITE; PS00081; LIPOXYGENASE_2; 1.
DR PROSITE; PS51393; LIPOXYGENASE_3; 1.
DR PROSITE; PS50095; PLAT; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR601885-2};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Iron {ECO:0000256|PIRSR:PIRSR601885-1};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR601885-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000007648}.
FT DOMAIN 2..117
FT /note="PLAT"
FT /evidence="ECO:0000259|PROSITE:PS50095"
FT DOMAIN 117..665
FT /note="Lipoxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51393"
FT BINDING 17
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-2"
FT BINDING 74
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-2"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-2"
FT BINDING 363
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-1"
FT BINDING 368
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-1"
FT BINDING 543
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-1"
FT BINDING 665
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-1"
FT SITE 102
FT /note="Essential for stabilizing binding to COTL1"
FT /evidence="ECO:0000256|PIRSR:PIRSR601885-3"
SQ SEQUENCE 665 AA; 75883 MW; 457FC21467D8496C CRC64;
MGRYRVRVAT GNSAFSGSNN QVQLWLVGLR AEVELGLRLR PARGQMEDFE VDVSEDLGPL
QFVKLRKIHL LIKDAWFCNH ITVWVFEQSM EEKEIVFPCY RWVEGDGIIC LPETTAWMIR
NESQGLFQKH QEEELKARRT LYGWVSWKKG IPLSVAAASE KDLPLDARFH EEKKVDFERT
RVVGVTEIAL KRLYTLLSSW NSLEDFDQIF WGGKSALAKR VRLSWKDDDF FGYQFLNGVN
PTLLRKSSSL PTRLVIPPGT EDLKAQLERK LQEGCLFEVD FSLLAGIQAN VIQGERQHLA
APLLMLKMEP DGKLLPMVIQ LQLPGSDSPV PPLFLPSDPS LTWLLAKAWV KNSDFQLHEL
QAHLLKTHLM AEVFAVATMR CLPGMHPIFK LLIPHLRYTL EINTRARTKL ISNGGFFDQA
VNTGGGGHLD VLRRATTCLT YRSLCPPDDL ADRGLLGVPS AFYAHDAVRL WDIISRYVQG
IVQLHYLEDE VVRSDPELQA WCREITEIGL CKAQERGFPV SFQSRDELCH FVTMCIFTCT
AQHSAVNQGQ LDWYTWVPNA PCTMRQPPPT TKDVTLEMVM ATMPNVHQAC IQMTISWHLG
RFQPDMVPLG HHKEQYFSGP GPQAVLNRFQ EDLYTLEREI VARNESLDLP YEYLKPSRIE
NSITI
//
