ID G3VLF7_SARHA Unreviewed; 605 AA.
AC G3VLF7;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 16-NOV-2011, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=glutaminase {ECO:0000256|ARBA:ARBA00012918};
DE EC=3.5.1.2 {ECO:0000256|ARBA:ARBA00012918};
GN Name=GLS2 {ECO:0000313|Ensembl:ENSSHAP00000004012.1};
OS Sarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Metatheria; Dasyuromorphia; Dasyuridae; Sarcophilus.
OX NCBI_TaxID=9305 {ECO:0000313|Ensembl:ENSSHAP00000004012.1, ECO:0000313|Proteomes:UP000007648};
RN [1] {ECO:0000313|Ensembl:ENSSHAP00000004012.1, ECO:0000313|Proteomes:UP000007648}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21709235; DOI=10.1073/pnas.1102838108;
RA Miller W., Hayes V.M., Ratan A., Petersen D.C., Wittekindt N.E., Miller J.,
RA Walenz B., Knight J., Qi J., Zhao F., Wang Q., Bedoya-Reina O.C.,
RA Katiyar N., Tomsho L.P., Kasson L.M., Hardie R.A., Woodbridge P.,
RA Tindall E.A., Bertelsen M.F., Dixon D., Pyecroft S., Helgen K.M.,
RA Lesk A.M., Pringle T.H., Patterson N., Zhang Y., Kreiss A., Woods G.M.,
RA Jones M.E., Schuster S.C.;
RT "Genetic diversity and population structure of the endangered marsupial
RT Sarcophilus harrisii (Tasmanian devil).";
RL Proc. Natl. Acad. Sci. U.S.A. 108:12348-12353(2011).
RN [2] {ECO:0000313|Ensembl:ENSSHAP00000004012.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001062};
CC -!- SIMILARITY: Belongs to the glutaminase family.
CC {ECO:0000256|ARBA:ARBA00011076}.
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DR RefSeq; XP_003770941.1; XM_003770893.2.
DR AlphaFoldDB; G3VLF7; -.
DR STRING; 9305.ENSSHAP00000004012; -.
DR Ensembl; ENSSHAT00000004052.2; ENSSHAP00000004012.1; ENSSHAG00000003536.2.
DR GeneID; 100931861; -.
DR KEGG; shr:100931861; -.
DR CTD; 27165; -.
DR eggNOG; KOG0506; Eukaryota.
DR GeneTree; ENSGT00390000010463; -.
DR HOGENOM; CLU_016439_1_0_1; -.
DR InParanoid; G3VLF7; -.
DR OMA; CISARPC; -.
DR OrthoDB; 537490at2759; -.
DR TreeFam; TF313359; -.
DR Proteomes; UP000007648; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-EC.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR GO; GO:0072593; P:reactive oxygen species metabolic process; IEA:Ensembl.
DR GO; GO:0042981; P:regulation of apoptotic process; IEA:Ensembl.
DR Gene3D; 1.10.238.210; -; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR HAMAP; MF_00313; Glutaminase; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR015868; Glutaminase.
DR InterPro; IPR041541; Glutaminase_EF-hand.
DR NCBIfam; TIGR03814; Gln_ase; 1.
DR PANTHER; PTHR12544; GLUTAMINASE; 1.
DR PANTHER; PTHR12544:SF33; GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF17959; EF-hand_14; 1.
DR Pfam; PF04960; Glutaminase; 1.
DR SMART; SM00248; ANK; 2.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
PE 3: Inferred from homology;
KW ANK repeat {ECO:0000256|ARBA:ARBA00023043, ECO:0000256|PROSITE-
KW ProRule:PRU00023}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000007648};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 76..158
FT /note="Glutaminase EF-hand"
FT /evidence="ECO:0000259|Pfam:PF17959"
FT REPEAT 521..543
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 605 AA; 66855 MW; CC06B195B561B27F CRC64;
MRSLRALRGA LHGPGGRPPG RGGWGPLARG PLLTRSVRHH LNEAAATQGR EMLNSHQPQH
QKEHDSSESG MLSRLGDLLF YTIAEGQERI AIHKFTAALR ATGLQTSDPR LRDCMSQMRR
MVRESSSGGL LDRDLFRKCV SSNIVLLTQA FRKKFVIPDF EEFTDHVDRI FEETKERTGG
KVAAYIPQLA KSDPDLWGVS LCTVDGQRHS VGQTKIPFCL QSCVKPLTYA ISVSNLGTEY
VHKYVGKEPS GLRYNKLSLN EEGIPHNPMV NAGAIVVSSL IKMDCNKAEK FDFVLQYLNK
MAGNEYMGFS NATFQSEKET GDRNYAIGYY LKEKKCFPKG VDMMAALDLY FQLCSVEVTC
ESGSVMAATL ANGGICPITG ESVLSAEAVR NTLSLMHSCG MYDFSGQFAF HVGLPAKSAV
SGAILLVVPN VMGLMCLSPP LDKVGNSHRG ISFCQKLVSL FNFHNYDNLR HCAKKLDPRR
EGGEVRNKTV VNLLFAAYSG DVSALRRFAL SAMDMEQKDY DSRTALHVAA AEGHLEVVKF
LVEACKVNPF VKDRWGNIPL DDAVQFNHLE VVKLLQDYQD SYTLSETQAE AAADALSKEN
LESMV
//
