ID G3WDA4_SARHA Unreviewed; 1190 AA.
AC G3WDA4;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 2.
DT 27-MAR-2024, entry version 67.
DE SubName: Full=NPC intracellular cholesterol transporter 1 {ECO:0000313|Ensembl:ENSSHAP00000013409.2};
GN Name=NPC1 {ECO:0000313|Ensembl:ENSSHAP00000013409.2};
OS Sarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Metatheria; Dasyuromorphia; Dasyuridae; Sarcophilus.
OX NCBI_TaxID=9305 {ECO:0000313|Ensembl:ENSSHAP00000013409.2, ECO:0000313|Proteomes:UP000007648};
RN [1] {ECO:0000313|Ensembl:ENSSHAP00000013409.2, ECO:0000313|Proteomes:UP000007648}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21709235; DOI=10.1073/pnas.1102838108;
RA Miller W., Hayes V.M., Ratan A., Petersen D.C., Wittekindt N.E., Miller J.,
RA Walenz B., Knight J., Qi J., Zhao F., Wang Q., Bedoya-Reina O.C.,
RA Katiyar N., Tomsho L.P., Kasson L.M., Hardie R.A., Woodbridge P.,
RA Tindall E.A., Bertelsen M.F., Dixon D., Pyecroft S., Helgen K.M.,
RA Lesk A.M., Pringle T.H., Patterson N., Zhang Y., Kreiss A., Woods G.M.,
RA Jones M.E., Schuster S.C.;
RT "Genetic diversity and population structure of the endangered marsupial
RT Sarcophilus harrisii (Tasmanian devil).";
RL Proc. Natl. Acad. Sci. U.S.A. 108:12348-12353(2011).
RN [2] {ECO:0000313|Ensembl:ENSSHAP00000013409.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol(in) = cholesterol(out); Xref=Rhea:RHEA:39747,
CC ChEBI:CHEBI:16113; Evidence={ECO:0000256|ARBA:ARBA00034049};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the patched family.
CC {ECO:0000256|ARBA:ARBA00005585}.
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DR AlphaFoldDB; G3WDA4; -.
DR STRING; 9305.ENSSHAP00000013409; -.
DR Ensembl; ENSSHAT00000013520.2; ENSSHAP00000013409.2; ENSSHAG00000011464.2.
DR eggNOG; KOG1933; Eukaryota.
DR GeneTree; ENSGT00940000156182; -.
DR HOGENOM; CLU_002359_0_0_1; -.
DR TreeFam; TF300416; -.
DR Proteomes; UP000007648; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005319; F:lipid transporter activity; IEA:InterPro.
DR GO; GO:0030301; P:cholesterol transport; IEA:UniProt.
DR Gene3D; 1.20.1640.10; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR InterPro; IPR004765; NPC1-like.
DR InterPro; IPR032190; NPC1_N.
DR InterPro; IPR003392; Ptc/Disp.
DR InterPro; IPR000731; SSD.
DR NCBIfam; TIGR00917; 2A060601; 1.
DR PANTHER; PTHR45727; NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1; 1.
DR PANTHER; PTHR45727:SF2; NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1; 1.
DR Pfam; PF16414; NPC1_N; 1.
DR Pfam; PF02460; Patched; 1.
DR Pfam; PF12349; Sterol-sensing; 1.
DR SUPFAM; SSF82866; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR PROSITE; PS50156; SSD; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Lipid transport {ECO:0000256|ARBA:ARBA00023055};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000007648};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 156..189
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 534..555
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 567..591
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 597..619
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 640..661
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 673..698
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1011..1030
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1037..1057
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1063..1081
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1102..1128
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1140..1163
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 533..698
FT /note="SSD"
FT /evidence="ECO:0000259|PROSITE:PS50156"
SQ SEQUENCE 1190 AA; 133259 MW; C6030AB86AFAEB35 CRC64;
MNLFCELTCS PRQSQFLNVT ATKSYIDPTT NETKTNVEEL QYYIGQSFAD AMYNACRDVE
SPSSNDKALG LLCGKDAKDC NATNWIEYMF NKNNGQAPFN IIPIFSDNSL YKMEPMNNAT
KGCNESVDDV TGPCSCQDCS ITCGPKPQPP PPPVPWLIFG LDAMYVIMWI FYMGFLLVFF
GMFFIVWCYR KRYFVSEYTP IDGNIAFSVN ASDRGMAWLL TSNLPSSPAL PGEASCGDRL
GAVFESCLTQ GFTRWGSFCV RNPVPVIICS LFFIAVCSSG LVFMRLATNP VDLWSAPNSQ
AHLEKEYFDT HFGPFFRTEQ LIIQAPHTKG HIYQPYPSGA DVPFGPPLDK GILHQVLDLQ
TAIENITASY NNETVTLEDI CLAPLSPYNN NCTILSVLNY FQNSHSVLDH QKGDEFFVYA
DYHTHFLYCT RAPASLNDTS LLHDSCLGTF GGPIFPWLVL GGYDDQNYNN ATALVITLPV
NNYYNNTEKL QKAQAWEKEF ISFVKNYKNP NLTISFSAER SIEDEINRES NGDVVTVLIS
YAVMFLYISI ALGHIKSFHR FLVDSKISLG IAGILMVLSS VVCSLGLFSY IGIPLTLIVI
EVIPFLVLAV GVDNIFILVQ TFQRDERLQG ETLDKQLGRI LGEVAPSMFL SSFSETIAFF
LGALSTMPAV RTFSLFAGMA VFIDFLLQIT CFVSLLGLDI KRQEKNKLDI LCCVRIAEDR
TDSQPSESYL FQFFKNAFSP FLLKDWMRPI VISVFVGILS FSIAVLNKVE IGLDQSLSMP
DDSYVLDYFK SLNQYLHAGP PVYFVLEEGH NYTSLEGQNM VCGGMGCNNN SLVQQIFNAA
ELENYTKIGF APSSWIDDYF DWIKPQSSCC RIYNMTERFC NASVVDPSCI HCRPLTPDGK
RRPQGEDFMK FLPMFLSDNP NPKCGKGGHA SYSAAVHFKN NYTEIGATYF MTYHTVLHTS
SDYIDAMRKA RMVAANITDT MGLQDKNYRV FPYSVFYVFY EQYLTIVDDT IFNLGVSLGA
IFLVTTILLG CDLWSSVIMC VTIAMILVNM FGVMWLWGIS LNAVSLVNLV MSCGISVEFC
SHITRAFTMS TKGSRVARAE EALSHMGSCV FSGITLTKFG GIVVLAFARS QIFQIFYFRM
YLAMVLLGAT HGLIFLPVLL SYIGPSVNKA KSRAAQERNR GTERERLLNF
//
