ID G3WJ74_SARHA Unreviewed; 520 AA.
AC G3WJ74;
DT 16-NOV-2011, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 2.
DT 27-MAR-2024, entry version 67.
DE SubName: Full=Pseudouridine synthase 1 {ECO:0000313|Ensembl:ENSSHAP00000015479.2};
GN Name=PUS1 {ECO:0000313|Ensembl:ENSSHAP00000015479.2};
OS Sarcophilus harrisii (Tasmanian devil) (Sarcophilus laniarius).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Metatheria; Dasyuromorphia; Dasyuridae; Sarcophilus.
OX NCBI_TaxID=9305 {ECO:0000313|Ensembl:ENSSHAP00000015479.2, ECO:0000313|Proteomes:UP000007648};
RN [1] {ECO:0000313|Ensembl:ENSSHAP00000015479.2, ECO:0000313|Proteomes:UP000007648}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21709235; DOI=10.1073/pnas.1102838108;
RA Miller W., Hayes V.M., Ratan A., Petersen D.C., Wittekindt N.E., Miller J.,
RA Walenz B., Knight J., Qi J., Zhao F., Wang Q., Bedoya-Reina O.C.,
RA Katiyar N., Tomsho L.P., Kasson L.M., Hardie R.A., Woodbridge P.,
RA Tindall E.A., Bertelsen M.F., Dixon D., Pyecroft S., Helgen K.M.,
RA Lesk A.M., Pringle T.H., Patterson N., Zhang Y., Kreiss A., Woods G.M.,
RA Jones M.E., Schuster S.C.;
RT "Genetic diversity and population structure of the endangered marsupial
RT Sarcophilus harrisii (Tasmanian devil).";
RL Proc. Natl. Acad. Sci. U.S.A. 108:12348-12353(2011).
RN [2] {ECO:0000313|Ensembl:ENSSHAP00000015479.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR AlphaFoldDB; G3WJ74; -.
DR STRING; 9305.ENSSHAP00000015479; -.
DR Ensembl; ENSSHAT00000015606.2; ENSSHAP00000015479.2; ENSSHAG00000013193.2.
DR eggNOG; KOG2553; Eukaryota.
DR GeneTree; ENSGT00950000183160; -.
DR InParanoid; G3WJ74; -.
DR OrthoDB; 12038at2759; -.
DR TreeFam; TF314367; -.
DR Proteomes; UP000007648; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:Ensembl.
DR GO; GO:0002153; F:steroid receptor RNA activator RNA binding; IEA:Ensembl.
DR GO; GO:0000049; F:tRNA binding; IEA:Ensembl.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IEA:Ensembl.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:Ensembl.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000007648};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 330..433
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 26..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 488..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..172
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 238
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 293
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 520 AA; 57893 MW; 41FB090AD6D0C975 CRC64;
MGRWGLRRGA RGCVGGGGGG GCAPGAGRAA GPGSRPPACP RMGSAWGSAA RRGGSARPRL
VPEVTRVGIQ SHLLSMGASL VKLEAVAQQS ARTRLLPLLH SELWHLRQVS MAEHLKAVPV
EAAPMKAEPG PMAKEDPNVR GSKDRGLKKW EETEPQAKRL KNNEEDEEHS KKLPKRKIVL
LMAYSGKGYH GMQRNLGSSQ FKTIEDDLVS ALVQSGCIPE NHGEDMKKMS FQRCARTDKG
VSAAGQIVSL KVWLIDNILE KINSHLPSHI RILGLKRVTG SFNSKNRCDA RTYFYMLPTF
AFAHKDHDIQ DERYRLSPET LEKVNQLLAG YKGTHNFHNF TSQKGPKEPS ARRYIMEMHC
EAPFMREGME FAVIKIKGQS FMMHQIRKMI GLVIAVLKGY VPESVMERSW GEEKVDIPKA
PGLGLVLERV HFEQYNRRFG NDGLHEPLDW VEEEEKMTVF KEQYIYPTII KTEMEEHSMT
NWLNTLPNHD FDAGSQEAKA DDGNTKAPAS LKGSDGGGDH
//
