ID G4E621_9GAMM Unreviewed; 756 AA.
AC G4E621;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN ORFNames=ThisiDRAFT_1750 {ECO:0000313|EMBL:EGZ45644.1};
OS Thiorhodospira sibirica ATCC 700588.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Thiorhodospira.
OX NCBI_TaxID=765914 {ECO:0000313|EMBL:EGZ45644.1};
RN [1] {ECO:0000313|EMBL:EGZ45644.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700588 {ECO:0000313|EMBL:EGZ45644.1};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Land M.L., Hauser L., Vogl K., Liu Z., Imhoff J.,
RA Frigaard N.-U., Bryant D., Woyke T.;
RT "The draft genome of Thiorhodospira sibirica ATCC 700588.";
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EGZ45644.1}.
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DR EMBL; AGFD01000032; EGZ45644.1; -; Genomic_DNA.
DR RefSeq; WP_006787766.1; NZ_MU255056.1.
DR AlphaFoldDB; G4E621; -.
DR STRING; 765914.ThisiDRAFT_1750; -.
DR PATRIC; fig|765914.3.peg.1736; -.
DR eggNOG; COG3605; Bacteria.
DR OrthoDB; 9765468at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244:SF1; PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EGZ45644.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 17..164
FT /note="GAF"
FT /evidence="ECO:0000259|SMART:SM00065"
FT COILED 406..433
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 756 AA; 83391 MW; CB8FAFFA5791FB15 CRC64;
MLDVLQRIVL EVNSAENLNQ ALEIIVRRVK LALDIDVCSV YLLLSEENRL VLLASDGLNP
DSIGRVKLNL GEGLVGVVAR RSEPLNLDNA PDHPAYKYFP ETGEECYHAF LGVPIINARR
MLGVLVVQQR EERRFRNEHV AFLVTLAAQL AGAISHAEMI GEIGAMQTQA RNAHVQVKGI
PGSSGIGIGV GVVAYSLADL DAVPDRLPSC IETEVTQFTQ AVEAVQNEME IIKQRMQDSL
PAEERLLFDA YIMMLGSESL FDRTVQGIRE GNKWAQAALR DTIRENTRSF EEMDDPYLRE
RASDLRDLGR RILSHLQAPQ RTPEDFPERT ILVGEDLTAT QLAEVPVHRL AGLVSSRGTS
SSHVAILARA MGVPAVMGMS DLPVTRLEGR QMIVDGYSGR IHIQPGRELL EEFRRLAREE
RQLSEELRAH SLEPAMTSDG FRIPVYANSG LLADMAPSLQ SGADGIGLYR TEVPFMIRDR
FPSEDEQTEI YREILTSLDP RPVTLRTLDV GGDKSLPYFP VKEDNPFLGW RGIRITLDHP
EIFLTQLRAM LRASQGLSNL HIMFPMVSSV SELKGALELL ARARDELLDE QLAILMPKTG
VMVEVPSAVY QAEALARRVD FLSVGTNDLV QYLLAVDRNN SRVAGIYDCL HPAVLQAMVQ
AACGAHRAGK TIGVCGEMAA DPAAVLLLLG MEMDSLSVSV SALARVKWVI RHFSRERTQE
LLEQSLCLDE PAAIRALLNN ALVKAGLGGL VRAGIH
//
