ID   G4E6A7_9GAMM            Unreviewed;       355 AA.
AC   G4E6A7;
DT   14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT   14-DEC-2011, sequence version 1.
DT   27-MAR-2024, entry version 32.
DE   RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN   ORFNames=ThisiDRAFT_1836 {ECO:0000313|EMBL:EGZ45099.1};
OS   Thiorhodospira sibirica ATCC 700588.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Thiorhodospira.
OX   NCBI_TaxID=765914 {ECO:0000313|EMBL:EGZ45099.1};
RN   [1] {ECO:0000313|EMBL:EGZ45099.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700588 {ECO:0000313|EMBL:EGZ45099.1};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Peters L., Land M.L., Hauser L., Vogl K., Liu Z., Imhoff J.,
RA   Frigaard N.-U., Bryant D., Woyke T.;
RT   "The draft genome of Thiorhodospira sibirica ATCC 700588.";
RL   Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Efflux system for nickel and cobalt.
CC       {ECO:0000256|ARBA:ARBA00002510}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC       Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC       {ECO:0000256|RuleBase:RU362101}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EGZ45099.1}.
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DR   EMBL; AGFD01000035; EGZ45099.1; -; Genomic_DNA.
DR   AlphaFoldDB; G4E6A7; -.
DR   STRING; 765914.ThisiDRAFT_1836; -.
DR   PATRIC; fig|765914.3.peg.1821; -.
DR   eggNOG; COG2215; Bacteria.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR   PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR   Pfam; PF03824; NicO; 1.
PE   3: Inferred from homology;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW   Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW   Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW   Nickel {ECO:0000256|ARBA:ARBA00022596};
KW   Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU362101};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU362101};
KW   Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           29..355
FT                   /note="Nickel/cobalt efflux system"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003462751"
FT   TRANSMEM        76..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        119..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        161..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        245..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        273..297
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
FT   TRANSMEM        318..340
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU362101"
SQ   SEQUENCE   355 AA;  38468 MW;  A0F2253833C6101E CRC64;
     MRIMRTWIKR LCVLVAIALL FIPLMTGAND SPTGYAPQAD DLSVWEQATQ WVLAKQRELH
     RELSTAMQAV REAPELATIT TLILISFFYG IFHAAGPGHG KAVITTYLLT HHQQLRRGVW
     LSFLSAIVQG VSAIVLVFVL VKLFGWLTRD ALAQADTVER FSFILVSLLG LWLVWRGGKP
     LWAHLRRPKP TPSTPPPAPA SARVIPIQAA PQQATPTPTH SAHCGCGNAH HIDPRTLDKA
     DRKTMLLVVL AVGMRPCTGA LVVLAVAHLL NLWLAGVMAV LAMSLGTAIT VSLLAVIAVK
     ARHLASRLVG GEHSRWPLLG HFAALAGGLL ILFIGLALLY GSTVMPPPPT HPFKL
//