ID G4RPU1_THETK Unreviewed; 244 AA.
AC G4RPU1;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Flavin-dependent thymidylate synthase {ECO:0000256|HAMAP-Rule:MF_01408};
DE Short=FDTS {ECO:0000256|HAMAP-Rule:MF_01408};
DE EC=2.1.1.148 {ECO:0000256|HAMAP-Rule:MF_01408};
DE AltName: Full=FAD-dependent thymidylate synthase {ECO:0000256|HAMAP-Rule:MF_01408};
DE AltName: Full=Thymidylate synthase ThyX {ECO:0000256|HAMAP-Rule:MF_01408};
DE Short=TS {ECO:0000256|HAMAP-Rule:MF_01408};
DE Short=TSase {ECO:0000256|HAMAP-Rule:MF_01408};
GN Name=thyX {ECO:0000256|HAMAP-Rule:MF_01408,
GN ECO:0000313|EMBL:CCC81586.1};
GN OrderedLocusNames=TTX_0934 {ECO:0000313|EMBL:CCC81586.1};
OS Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435
OS / Kra 1).
OC Archaea; Thermoproteota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Thermoproteus.
OX NCBI_TaxID=768679 {ECO:0000313|EMBL:CCC81586.1, ECO:0000313|Proteomes:UP000002654};
RN [1] {ECO:0000313|EMBL:CCC81586.1, ECO:0000313|Proteomes:UP000002654}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1
RC {ECO:0000313|Proteomes:UP000002654};
RX PubMed=22003381; DOI=10.1371/journal.pone.0024222;
RA Siebers B., Zaparty M., Raddatz G., Tjaden B., Albers S.V., Bell S.D.,
RA Blombach F., Kletzin A., Kyrpides N., Lanz C., Plagens A., Rampp M.,
RA Rosinus A., von Jan M., Makarova K.S., Klenk H.P., Schuster S.C.,
RA Hensel R.;
RT "The complete genome sequence of Thermoproteus tenax: a physiologically
RT versatile member of the Crenarchaeota.";
RL PLoS ONE 6:E24222-E24222(2011).
CC -!- FUNCTION: Catalyzes the reductive methylation of 2'-deoxyuridine-5'-
CC monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while
CC utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor,
CC and NADPH and FADH(2) as the reductant. {ECO:0000256|HAMAP-
CC Rule:MF_01408}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP + H(+) +
CC NADPH = (6S)-5,6,7,8-tetrahydrofolate + dTMP + NADP(+);
CC Xref=Rhea:RHEA:29043, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.148;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01408};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01408};
CC Note=Binds 4 FAD per tetramer. Each FAD binding site is formed by three
CC monomers. {ECO:0000256|HAMAP-Rule:MF_01408};
CC -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis. {ECO:0000256|HAMAP-
CC Rule:MF_01408}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01408}.
CC -!- SIMILARITY: Belongs to the thymidylate synthase ThyX family.
CC {ECO:0000256|HAMAP-Rule:MF_01408}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_01408}.
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DR EMBL; FN869859; CCC81586.1; -; Genomic_DNA.
DR RefSeq; WP_014126842.1; NC_016070.1.
DR AlphaFoldDB; G4RPU1; -.
DR STRING; 768679.TTX_0934; -.
DR PaxDb; 768679-TTX_0934; -.
DR GeneID; 11261827; -.
DR KEGG; ttn:TTX_0934; -.
DR PATRIC; fig|768679.9.peg.944; -.
DR eggNOG; arCOG01883; Archaea.
DR HOGENOM; CLU_1136110_0_0_2; -.
DR UniPathway; UPA00575; -.
DR Proteomes; UP000002654; Chromosome.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0050797; F:thymidylate synthase (FAD) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006231; P:dTMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006235; P:dTTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd20175; ThyX; 1.
DR Gene3D; 3.30.1360.170; -; 1.
DR HAMAP; MF_01408; ThyX; 1.
DR InterPro; IPR003669; Thymidylate_synthase_ThyX.
DR InterPro; IPR036098; Thymidylate_synthase_ThyX_sf.
DR PANTHER; PTHR34934; FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR34934:SF1; FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE; 1.
DR Pfam; PF02511; Thy1; 1.
DR SUPFAM; SSF69796; Thymidylate synthase-complementing protein Thy1; 1.
DR PROSITE; PS51331; THYX; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|HAMAP-Rule:MF_01408};
KW Flavoprotein {ECO:0000256|HAMAP-Rule:MF_01408};
KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_01408,
KW ECO:0000313|EMBL:CCC81586.1}; NADP {ECO:0000256|HAMAP-Rule:MF_01408};
KW Nucleotide biosynthesis {ECO:0000256|HAMAP-Rule:MF_01408};
KW Reference proteome {ECO:0000313|Proteomes:UP000002654};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_01408, ECO:0000313|EMBL:CCC81586.1}.
FT ACT_SITE 163
FT /note="Involved in ionization of N3 of dUMP, leading to its
FT activation"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 58
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /ligand_note="ligand shared between neighboring subunits"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 78..81
FT /ligand="dUMP"
FT /ligand_id="ChEBI:CHEBI:246422"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 81..83
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /ligand_note="ligand shared between neighboring subunits"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 91..93
FT /ligand="dUMP"
FT /ligand_id="ChEBI:CHEBI:246422"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 135
FT /ligand="dUMP"
FT /ligand_id="ChEBI:CHEBI:246422"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 151..153
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /ligand_note="ligand shared between neighboring subunits"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 158
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /ligand_note="ligand shared between neighboring subunits"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
FT BINDING 163
FT /ligand="dUMP"
FT /ligand_id="ChEBI:CHEBI:246422"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01408"
SQ SEQUENCE 244 AA; 28787 MW; 6C5963D6ACE5EF86 CRC64;
MTTSVRFVDP SVRLVGHWGS EELISSFVDV LYRGEERAQD PATVAKRIKM FYRLGHWSVF
EFMGAQFLVE CSRACHTQFI RHRLASYWSE SQRYVDYAKR PIRFIVPKGF PEEALRRAYE
DYLKLRELYP PEYARMALPN ATAVKFAVQM NARELLLNFV PLRCSAAAQA EIRHICWQMF
AHAWRLWPTL SRLVWDDLPN LHRDFCTKVP KGEDCRLYAI RDAEEKHGQL PEKPWLQRAS
VEYL
//