ID G4SWZ4_META2 Unreviewed; 432 AA.
AC G4SWZ4;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN Name=gltA {ECO:0000313|EMBL:CCE23049.1};
GN OrderedLocusNames=MEALZ_1360 {ECO:0000313|EMBL:CCE23049.1};
OS Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM
OS B-2133 / 20Z) (Methylomicrobium alcaliphilum).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylotuvimicrobium.
OX NCBI_TaxID=1091494 {ECO:0000313|EMBL:CCE23049.1, ECO:0000313|Proteomes:UP000008315};
RN [1] {ECO:0000313|Proteomes:UP000008315}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z
RC {ECO:0000313|Proteomes:UP000008315};
RX PubMed=22207753; DOI=10.1128/JB.06392-11;
RA Vuilleumier S., Khmelenina V.N., Bringel F., Reshetnikov A.S., Lajus A.,
RA Mangenot S., Rouy Z., Op den Camp H.J., Jetten M.S., Dispirito A.A.,
RA Dunfield P., Klotz M.G., Semrau J.D., Stein L.Y., Barbe V., Medigue C.,
RA Trotsenko Y.A., Kalyuzhnaya M.G.;
RT "Genome sequence of the haloalkaliphilic methanotrophic bacterium
RT Methylomicrobium alcaliphilum 20Z.";
RL J. Bacteriol. 194:551-552(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308,
CC ECO:0000256|RuleBase:RU003370};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC ECO:0000256|RuleBase:RU003370}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
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DR EMBL; FO082060; CCE23049.1; -; Genomic_DNA.
DR RefSeq; WP_014147845.1; NC_016112.1.
DR AlphaFoldDB; G4SWZ4; -.
DR STRING; 1091494.MEALZ_1360; -.
DR KEGG; mah:MEALZ_1360; -.
DR PATRIC; fig|271065.3.peg.1398; -.
DR HOGENOM; CLU_025068_0_0_6; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000008315; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 2.20.28.60; -; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR NCBIfam; TIGR01798; cit_synth_I; 1.
DR PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:CCE23049.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008315};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU003370}.
FT ACT_SITE 309
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 367
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 432 AA; 49083 MW; B78AA574B7BAA686 CRC64;
MTNHNQVTIR NEDDRISCDL PVLQGTIGPD VIDIRTLYRD TGVFTYDPGF TSTASCSSKI
TFIDGDKGIL LYRGYPIEQL AEKCDFMEVC YLLLHDELPN GRQMQEFVND ITYHTMVHDQ
LTDFYKGFRR DAHPMATIVA VVGALSAFYH HELDIRCPID RNLSAIRLIA KMPTIVAMSH
KYSSGMPFMY PQNKLGYVEN FMYMMHATPC EDYVQNPILV RAMDRILILH ADHEQNASTS
TVRLAGSSGA NPYACITSGI ACLWGAAHGG ANEAVLRMLL EIGDVSRIGE FIDKAKDKND
PFRLMGFGHR VYKNYDPRAK LMRETCHEVL NELGLHDDPL FKLALELERI ALEDEYFIEK
KLYPNVDFYS GIVMRALGIP TVMFTAIFAM ARSIGWIAHW DEMISDDEQK IGRPRQLYKG
HTQRDVVSIS AR
//
