ID G4YFA9_PHYSP Unreviewed; 817 AA.
AC G4YFA9;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 03-MAY-2023, entry version 41.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
GN ORFNames=PHYSODRAFT_471658 {ECO:0000313|EMBL:EGZ28335.1};
OS Phytophthora sojae (strain P6497) (Soybean stem and root rot agent)
OS (Phytophthora megasperma f. sp. glycines).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=1094619 {ECO:0000313|EMBL:EGZ28335.1, ECO:0000313|Proteomes:UP000002640};
RN [1] {ECO:0000313|EMBL:EGZ28335.1, ECO:0000313|Proteomes:UP000002640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P6497 {ECO:0000313|EMBL:EGZ28335.1,
RC ECO:0000313|Proteomes:UP000002640};
RX PubMed=16946064; DOI=10.1126/science.1128796;
RA Tyler B.M., Tripathy S., Zhang X., Dehal P., Jiang R.H., Aerts A.,
RA Arredondo F.D., Baxter L., Bensasson D., Beynon J.L., Chapman J.,
RA Damasceno C.M., Dorrance A.E., Dou D., Dickerman A.W., Dubchak I.L.,
RA Garbelotto M., Gijzen M., Gordon S.G., Govers F., Grunwald N.J., Huang W.,
RA Ivors K.L., Jones R.W., Kamoun S., Krampis K., Lamour K.H., Lee M.K.,
RA McDonald W.H., Medina M., Meijer H.J., Nordberg E.K., Maclean D.J.,
RA Ospina-Giraldo M.D., Morris P.F., Phuntumart V., Putnam N.H., Rash S.,
RA Rose J.K., Sakihama Y., Salamov A.A., Savidor A., Scheuring C.F.,
RA Smith B.M., Sobral B.W., Terry A., Torto-Alalibo T.A., Win J., Xu Z.,
RA Zhang H., Grigoriev I.V., Rokhsar D.S., Boore J.L.;
RT "Phytophthora genome sequences uncover evolutionary origins and mechanisms
RT of pathogenesis.";
RL Science 313:1261-1266(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR EMBL; JH159151; EGZ28335.1; -; Genomic_DNA.
DR RefSeq; XP_009515610.1; XM_009517315.1.
DR AlphaFoldDB; G4YFA9; -.
DR STRING; 1094619.G4YFA9; -.
DR EnsemblProtists; EGZ28335; EGZ28335; PHYSODRAFT_471658.
DR GeneID; 20654127; -.
DR KEGG; psoj:PHYSODRAFT_471658; -.
DR InParanoid; G4YFA9; -.
DR OMA; SALRICW; -.
DR Proteomes; UP000002640; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR Gene3D; 1.10.720.30; SAP domain; 1.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR003034; SAP_dom.
DR InterPro; IPR036361; SAP_dom_sf.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR Pfam; PF02037; SAP; 1.
DR SMART; SM00513; SAP; 1.
DR SUPFAM; SSF68906; SAP domain; 1.
DR PROSITE; PS50800; SAP; 1.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000002640}.
FT DOMAIN 525..559
FT /note="SAP"
FT /evidence="ECO:0000259|PROSITE:PS50800"
FT DOMAIN 633..732
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
SQ SEQUENCE 817 AA; 90544 MW; 1924DE03255E1591 CRC64;
MLGALKATTK QRSTATVVKA LGLREMLVHT ILSDIHFREG RSTGAIIEAK SALRICWKMA
KRFTAASSTV EAAYFDLPSE LSATDWQHRK SAHLYFMALE SSSWDVLYAA KVSLCRIASL
YCYSDQPHRA ASYLTEAMRL VGGLNLHLFR RTPFYEFAEL ELNASHLEKA KTAISMLSSN
SRMEPSSALA MVNARCWRKY TRHHSEIIDL SDTSAVETLL NSMKTLKRTL KRCGARLERV
KCMLELGRIN IKLLRSSSCR AFASLERTIS LLEEAYSLGD CLGIAHLSQE LRATLGMAYF
AEIEESAHGD ADRMDGDRAR FLSWSSSALL ANSCSSTECE MVHELPSSWV IVSLMMGLSS
ELTNGTAPIS FCLPNVSWKS CIDKMDAIVE KSREILSGHT AEEAGSWSTK QKKEWWDTRK
QLDERIQKSV RSMEMTLGFW RCLLVKGTST LETQCVQQCW SLLTSRKVCP VLLAERNQSL
LCAIADAQQW LSDGEVVNGL KHIAAEIDVP LSDSGLQEAL QTDKIQKMKV SELKQLLAAE
GLSTDGLKKV LVERLIAARD AALLEPLTDQ AARDQGFSTI LILNHQLQQF PWEGMDIMGR
SSGVTRMPSL DLTMQNARYV MQSRTSSYSS IRRDRVRFLL NPAGDLKATQ NQLGPVLEGG
VTTYGWEGIV GEAPDPDKLR NYLLAADLYI YCGHGSGEAY LHRDQVLSLK SGCSAALLFG
CSSGRLKREG IFGPSGAVLA YLRAGSPAVL AMLWDVTDRD IDQLSVKILR EWLLSEDGDS
SPSLARVLQD SRSVCKLKYL NGHAAVCYGL PLHVAKS
//
