ID G5B7Z7_HETGA Unreviewed; 1000 AA.
AC G5B7Z7;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=DNA replication ATP-dependent helicase/nuclease DNA2 {ECO:0000256|ARBA:ARBA00021516};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
DE AltName: Full=DNA replication ATP-dependent helicase-like homolog {ECO:0000256|ARBA:ARBA00032548};
GN ORFNames=GW7_18629 {ECO:0000313|EMBL:EHB05408.1};
OS Heterocephalus glaber (Naked mole rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Bathyergidae;
OC Heterocephalus.
OX NCBI_TaxID=10181 {ECO:0000313|EMBL:EHB05408.1, ECO:0000313|Proteomes:UP000006813};
RN [1] {ECO:0000313|EMBL:EHB05408.1, ECO:0000313|Proteomes:UP000006813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993625; DOI=10.1038/nature10533;
RA Kim E.B., Fang X., Fushan A.A., Huang Z., Lobanov A.V., Han L.,
RA Marino S.M., Sun X., Turanov A.A., Yang P., Yim S.H., Zhao X.,
RA Kasaikina M.V., Stoletzki N., Peng C., Polak P., Xiong Z., Kiezun A.,
RA Zhu Y., Chen Y., Kryukov G.V., Zhang Q., Peshkin L., Yang L., Bronson R.T.,
RA Buffenstein R., Wang B., Han C., Li Q., Chen L., Zhao W., Sunyaev S.R.,
RA Park T.J., Zhang G., Wang J., Gladyshev V.N.;
RT "Genome sequencing reveals insights into physiology and longevity of the
RT naked mole rat.";
RL Nature 479:223-227(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
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DR EMBL; JH168904; EHB05408.1; -; Genomic_DNA.
DR AlphaFoldDB; G5B7Z7; -.
DR STRING; 10181.G5B7Z7; -.
DR eggNOG; KOG1805; Eukaryota.
DR InParanoid; G5B7Z7; -.
DR Proteomes; UP000006813; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017116; F:single-stranded DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:UniProtKB-UniRule.
DR CDD; cd18041; DEXXQc_DNA2; 1.
DR CDD; cd22318; DNA2_N-like; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR026851; Dna2/JHS1_DEXXQ-box.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR048459; DNA2_Rift.
DR InterPro; IPR014808; DNA_replication_fac_Dna2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR36531; CRISPR-ASSOCIATED EXONUCLEASE CAS4; 1.
DR PANTHER; PTHR36531:SF6; DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF08696; Dna2; 1.
DR Pfam; PF21123; Dna2_Rift; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:EHB05408.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000006813}.
FT DOMAIN 76..280
FT /note="DNA replication factor Dna2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08696"
FT DOMAIN 469..566
FT /note="DNA2 rift barrel"
FT /evidence="ECO:0000259|Pfam:PF21123"
FT DOMAIN 626..724
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 730..797
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 870..957
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
SQ SEQUENCE 1000 AA; 113411 MW; C317F8C368C27024 CRC64;
MEQRDELELL MDESFEEEAA PPAELFQKKL EASFPKTILS QGTDNRYLVL AVDIVQNKGS
YEKHLIITAS WSLECKEQCI LRNDWCSVPV QPGDIIHLEG DCSFDTWIID KDFGFLILYP
DMLISGTSIA SSIRCMRRAV LSETFRSSDQ ATRQMLMGTV LHEVFQKAIS DSFAPEKLKE
LAFQTVQEIR HLKEMYRLNL SQDEIKQEAE EYLPSFSKWA GDFMRKSASA DFPQMQLSLP
SDGSNNNSTC SIEVVSSLDI EESIWSPRFG MKGKIDVTVG VKIHRGCKAK YKIMPLELKT
GKESNSIEHR GQVVLYTLLS QERRADPEAG LLLYLKTGQM YPVAAKHLDK RELLKLRNQM
AFSLLHRTSK SATGKEKTQL ASLPQIIEEE KICKYCSQMN NCALYSRAVE QQMEDVSVPV
VMLPRIKEAT KHLKLTHLQY FSVWCLMLTL ESQSKDSRRN HQNIWLMSAS EMEESGSCIG
YLIRTECVQR VCDGQYIHNF QRKHGAMPVT NLMAGDRIIL SGEDRTLFAL SRGYVKEINM
TTITCLLDRN LSVLPESTLF RLDQEEKNCD IDTPLGNLSK LMENTQASKK LRDLIIDFRE
PQFIPYLSSV LPHDAKDTVA SILKGLNKPQ RQAMKKVLLS KDYTLIVGMP GTGKTTTICT
LVRILYACGF SVLLTSYTHS AVDNILLKLA KFKIGFLRLG QTQKVHPDIQ KFTEEEICRS
KSIKSLAVLE ELYNSQLIVA TTCMGINHPI FSRKTFDFCI VDEASQISQP VCLGPLFFSQ
RFVLVGDHQQ LPPLVLNREA SKIMSLSNEL TYEGKLECGS DRVASGVISL PNFKDVKLEL
ELYADYSDSL WLNGVFEPNN PVCFLNTDKA GCNPSDIGII APYRQQLKII SDLLTRSSFG
MVEVNTVDKY QGRDKRIILV SFVRSNKNGT LGELLKDWRR LNVAITRAKH KLILLGCVPS
LKRYPPLEKL FCHLNSEKLI SFLSCIEHES LCHILGDFQR
//
